scholarly journals Comparison between the reference Rhizobium tropici CIAT899 and the native Rhizobium etli 12a3 for some nitrogen fixation parameters in common bean (Phaseolus vulgaris L.) under water stress

2012 ◽  
Vol 6 (18) ◽  
Author(s):  
Fatma Tajini
Keyword(s):  
Nitrogen Fixation ◽  
Water Stress ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Rhizobium Etli ◽  
Phaseolus Vulgaris L ◽  
Rhizobium Tropici

A 150-megadalton plasmid in Rhizobium etli strain TAL182 contains genes for nodulation competitiveness on Phaseolus vulgaris L.

1996 ◽  
Vol 42 (9) ◽  
pp. 903-910 ◽  
Author(s):  
Dulal Borthakur ◽  
Xuefeng Gao
Keyword(s):  
Nitrogen Fixation ◽  
Phaseolus Vulgaris ◽  
Genomic Dna ◽  
Functional Complementation ◽  
Rhizobium Etli ◽  
Phaseolus Vulgaris L ◽  
Rhizobium Tropici ◽  
Hindiii Fragment ◽  
Nodulation Competitiveness ◽  
Insertion Mutants

Rhizobium etli TAL182, a competitive strain for the nodulation of Phaseolus beans, occupied more than 99% of the nodules when co-inoculated in various proportions with Rhizobium TAL1145 or Rhizobium tropici CIAT899. Two overlapping cosmid clones, pUHR68 and pUHR69, containing genes for nodulation competitiveness from TAL182, were isolated by functional complementation of strain TAL1145. Using one of these cosmid clones, we constructed two Tn5-insertion mutants of TAL182 defective in nodulation competitiveness. The Tn5 insertions in both mutants were localized in identical positions within a 4.6-kb HindIII fragment. One mutant, RUH120, was complemented for nodulation competitiveness by this HindIII fragment. The cloned DNA in pUHR68 is a part of a plasmid, 150 MDa in size, in TAL182 and does not show homology with TAL1145 genomic DNA. The 4.6-kb HindIII fragment contains a gene(s) required for nodulation competitiveness on beans, which is present only in some R. etli strains and absent in other Rhizobium spp.Key words: nitrogen fixation, competition, nodule, common bean.

scholarly journals Effect of inoculation associated to leaf sprayed Co+Mo on the yield and grain nutrients in common bean (Phaseolus vulgaris L)

2008 ◽  
Vol 51 (6) ◽  
pp. 1089-1096 ◽  
Author(s):  
João Francisco Berton Junior ◽  
Julio Cesar Pires Santos ◽  
Cileide Maria Medeiros Coelho ◽  
Osmar Klauberg Filho
Keyword(s):  
Phaseolus Vulgaris ◽  
Grain Yield ◽  
Common Bean ◽  
Protein Content ◽  
Nitrogen Source ◽  
Mineral Nitrogen ◽  
Nitrogen Fixing ◽  
Phaseolus Vulgaris L ◽  
Rhizobium Tropici ◽  
The Common

The objective of this work was to evaluate the efficiency of nitrogen fixing inoculum associated with Co + Mo leaf spray on the common bean grain yield and grain nutrients, cv. FT Nobre. Three dosages of the inoculant (0, 200 and 400 g/50 kg seeds), combined with four Co + Mo leaf spray levels (T0=0,0; T1=4.9,49; T2=7.3,73; and T3=9.7,97 g ha-1 of Co and Mo, respectively) were tested. The grain yield with the use of the inoculant (400 g / 5O kg seed-1) associated with the higher level of Co+Mo (T2 and T3) was very similar to the mineral nitrogen condition fertilizer recommended for the bean (70 kg ha-1 of N). With the increased inoculant dosage, an increase of the protein content and of P and Mg in the grain was also observed. The results indicated that the mineral nitrogen source could be replaced by inoculation of the seeds with Rhizobium tropici combined with Co + Mo leaf spray.

Analysis of the Proteome of Common Bean (Phaseolus vulgaris L.) Roots after Inoculation with Rhizobium etli

2012 ◽  
Vol 19 (8) ◽  
pp. 880-889 ◽  
Author(s):  
Afshin Salavati ◽  
Alireza Taleei ◽  
Ali Akbar Shahnejat Bushehri ◽  
Setsuko Komatsu
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Rhizobium Etli ◽  
Phaseolus Vulgaris L

RHIZOBIAL INOCULATION AND P FERTILIZATION RESPONSE IN COMMON BEAN (PHASEOLUS VULGARIS) UNDER GLASSHOUSE AND FIELD CONDITIONS

2007 ◽  
Vol 43 (1) ◽  
pp. 67-77 ◽  
Author(s):  
M. ZAMAN-ALLAH ◽  
B. SIFI ◽  
B. L'TAIEF ◽  
M. H. EL AOUNI ◽  
J. J. DREVON
Keyword(s):  
Nitrogen Fixation ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Symbiotic Nitrogen Fixation ◽  
Field Conditions ◽  
Nutrient Deficiencies ◽  
P Fertilization ◽  
Rhizobium Tropici ◽  
Symbiotic Efficiency ◽  
Rhizobial Inoculation

Osmotic constraints, nutrient deficiencies, especially phosphorus, and the lack of efficient strains of rhizobia in Mediterranean soils are major factors limiting symbiotic nitrogen fixation and yield in common bean (Phaseolus vulgaris). In order to improve yields, we investigated responses to rhizobial inoculation and P fertilization under glasshouse and field conditions with two bean cultivars, Coco blanc and BAT477. The nodulation test, using a hydroponic system in a glasshouse, revealed strong variability among 22 rhizobium strains of various origins, in their symbiotic efficiency, compared with Rhizobium tropici CIAT899. The introduced strains proved to be more efficient although some local rhizobia revealed high potential efficiencies. Glasshouse trials showed a significant effect of inoculation and P supply on nodulation, N content and shoot dry weight that varied with rhizobial strain. Field trials substantiated the glasshouse observations and emphasized the effects of cultivar-rhizobia interactions on symbiotic parameters and yields. It is concluded that inoculation with suitable rhizobia with supply of additional P is a technology that may improve symbiotic nitrogen fixation and yield in common bean in some soils under a Mediterranean climate.

Comparison of common bean (Phaseolus vulgaris L.) genotypes for nitrogen fixation tolerance to soil drying

2012 ◽  
Vol 364 (1-2) ◽  
pp. 29-37 ◽  
Author(s):  
Mura Jyostna Devi ◽  
Thomas R. Sinclair ◽  
Stephen E. Beebe ◽  
Idupulapati M. Rao
Keyword(s):  
Nitrogen Fixation ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Soil Drying ◽  
Phaseolus Vulgaris L
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