Enhancement of diosgenin production in Dioscorea zingiberensis seedling and cell cultures by beauvericin from the endophytic fungus Fusarium redolens Dzf2

2011 ◽  
Vol 5 (29) ◽  
Author(s):  
Chunhui Yin
Keyword(s):  
Cell Cultures ◽  
Endophytic Fungus ◽  
Dioscorea Zingiberensis ◽  
Fusarium Redolens

scholarly journals Beauvericin from the Endophytic Fungus, Fusarium redolens, Isolated from Dioscorea zingiberensis and Its Antibacterial Activity

2010 ◽  
Vol 5 (5) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Lijian Xu ◽  
Jihua Wang ◽  
Jianglin Zhao ◽  
Peiqin Li ◽  
Tijiang Shan ◽  
...  
Keyword(s):  
Endophytic Fungus ◽  
Inhibitory Concentration ◽  
Fungal Endophyte ◽  
Dioscorea Zingiberensis ◽  
Antibacterial Assay ◽  
Fusarium Redolens ◽  
Potential Use ◽  
Pseudomonas Lachrymans ◽  
Xanthomonas Vesicatoria ◽  
Chinese Medicinal Plant

From the fungal endophyte, Fusarium redolens Dzf2, isolated from rhizomes of the Chinese medicinal plant Dioscorea zingiberensis, beauvericin was obtained by TLC in combination with bioautographic antibacterial assay. The compound was identified by spectroscopic and physicochemical means. The median effective inhibitory concentration (IC50) values of beauvericin against six test bacteria ( Bacillus subtilis, Staphylococcus haemolyticus, Pseudomonas lachrymans, Agrobacterium tumefaciens, Escherichia coli and Xanthomonas vesicatoria) were between 18.4 and 70.7 μg/mL. The beauvericin content of F. redolens Dzf2 mycelia was 9.60 mg/g dw, and beauvericin yield 62.4 mg/L. The obtained results show the potential use of the endophytic fungus for its biological role in providing its host plant with protection, as well as the possible development of beauvericin as an antibacterial.

Beauvericin produced by an endophytic fungus Fusarium redolens isolated from Dioscorea zingiberensis and its antibacterial activity

2008 ◽  
Vol 136 ◽  
pp. S143 ◽  
Author(s):  
Lijian Xu ◽  
Jianglin Zhao ◽  
Yongfu Huang ◽  
Youliang Peng ◽  
Jianyong Wu ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungus ◽  
Dioscorea Zingiberensis ◽  
Fusarium Redolens

scholarly journals Enhancement of Diosgenin Production in Dioscorea zingiberensis Cell Cultures by Oligosaccharides from Its Endophytic Fungus Fusarium oxysporum Dzf17

Molecules ◽  
2011 ◽  
Vol 16 (12) ◽  
pp. 10631-10644 ◽  
Author(s):  
Peiqin Li ◽  
Ziling Mao ◽  
Jingfeng Lou ◽  
Yan Li ◽  
Yan Mou ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Cell Cultures ◽  
Endophytic Fungus ◽  
Dioscorea Zingiberensis

scholarly journals Enhancement of Diosgenin Production in Dioscorea zingiberensis Cell Culture by Oligosaccharide Elicitor from its Endophytic Fungus Fusarium oxysporum Dzf17

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401
Author(s):  
Ruifen Zhang ◽  
Peiqin Li ◽  
Lijian Xu ◽  
Yuanquan Chen ◽  
Peng Sui ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Fusarium Oxysporum ◽  
Cell Suspension Culture ◽  
Endophytic Fungus ◽  
Cultured Cells ◽  
Cell Suspension Cultures ◽  
Fungal Cell ◽  
Partial Acid Hydrolysis ◽  
Dioscorea Zingiberensis

Diosgenin accumulation in cell suspension cultures of Dioscorea zingiberensis C. H. Wright was enhanced by treatment with saccharide elicitors from its endophytic fungus Fusarium oxysporum isolate Dzf17. The crude oligosaccharide was prepared by partial acid hydrolysis of the isolated Dzf17 fungal cell wall fragments. Optimal elicitation of diosgenin production by the isolated Dzf17 oligosaccharide in cell suspension culture was achieved when the oligosaccharide was added to the medium at a concentration of 30 mg/L after 16-day's continuous cell suspension culture, and the cells were cultured for another 8 days before harvesting. By using these optimal conditions, the diosgenin yield of the cultured cells reached its maximum of 5.25 mg/L, which was over a three-fold increase.

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