Antimicrobial susceptibility profile in vitro of milk samples from cows from a small property in Guatapará, São Paulo State

This paper reports the occurrence of an megabacterium-like organism at small birds from the Northeast area of São Paulo State - Brazil. The results presented herein were obtained from 64 necropsy along four years (1994-1997). Sixty four birds (4 budgerigars Melopsittacus undulatus, 12 lovebirds Agapornis spp and 48 canaries Serinus canaria) were studied. At 56% of the examined birds showed at the proventricular mucus smear a presence of rod-shaped (similar to a toothpick) organisms, Gram positive and acidophilic in Giemsa staining, called megabacteria. Different kind of culture media was testes to replicated these organism in vitro. Also the dimension (large and width) of the fresh microorganism from the proventricular mucus and from the first culture was compared. The macroscopic alterations observed at the necropsies was also listed.

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In vitro and in vivo investigation of the genotoxic potential of waters from rivers under the influence of a petroleum refinery (São Paulo State – Brazil)

Chemosphere ◽

10.1016/j.chemosphere.2017.01.142 ◽

2017 ◽

Vol 174 ◽

pp. 321-330 ◽

Cited By ~ 13

Author(s):

Raquel Vaz Hara ◽

Maria Aparecida Marin-Morales

Keyword(s):

Petroleum Refinery ◽

Sao Paulo ◽

São Paulo ◽

Paulo State ◽

Genotoxic Potential ◽

São Paulo State

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Environmental parameters and antimicrobial susceptibility of enterobacteriaceae isolated from estuarine waters of São Vicente, São Paulo state, Brazil

Journal of Venomous Animals and Toxins including Tropical Diseases ◽

10.1590/s1678-91992007000200006 ◽

2007 ◽

Vol 13 (2) ◽

Cited By ~ 1

Author(s):

N. G. G. S. Lourenço ◽

C. K. Takahashi ◽

T. F. Lopes ◽

C. A. M. Lopes

Keyword(s):

Antimicrobial Susceptibility ◽

Environmental Parameters ◽

Sao Paulo ◽

São Paulo ◽

Estuarine Waters ◽

Paulo State ◽

São Paulo State

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Pheno and genotyping ofStaphylococcus aureus, isolated from bovine milk samples from São Paulo State, Brazil

Canadian Journal of Microbiology ◽

10.1139/w04-082 ◽

2004 ◽

Vol 50 (11) ◽

pp. 901-909 ◽

Cited By ~ 17

Author(s):

K G Cabral ◽

C Lämmler ◽

M Zschöck ◽

H Langoni ◽

M E.P de Sá ◽

...

Keyword(s):

Bovine Milk ◽

Bovine Mastitis ◽

Sao Paulo ◽

Surface Proteins ◽

São Paulo ◽

Ofstaphylococcus Aureus ◽

Pfge Analysis ◽

Paulo State ◽

Milk Samples ◽

São Paulo State

In the present study, 87 Staphylococcus aureus isolates obtained from milk samples of 87 cows with mastitis in 6 different municipal districts of 2 regions of São Paulo State, Brazil, were compared pheno and genotypically. Pulsed-field gel electrophoresis (PFGE) analysis of the strains was performed, and PCR was carried out to detect genes for a number of staphylococcal cell surface proteins, exoproteins, and 3 classes of agr genes. Nine distinct S. aureus lineages (LA–LI) were identified by PFGE. The lineages LA and LE, which accounted together for 63 strains (72.2%), were prevalent and had been collected from all of the 6 municipal districts, indicating a broad geographic distribution of these lineages; LB, LC, LD, LF, LG, LH, and LI, however, were isolated sporadically and accounted for 24 strains (27.8%). Some characteristics, like penicillin resistance and the presence of cap8 and agr class II genes, were associated with the prevalent lineages (LA and LE), and penicillin susceptibility and the presence of cna and cap5 genes were associated with sporadic lineages. According to the present results, some S. aureus lineages possess a combination of genes that confer the propensity to cause and disseminate infection, and only a limited number of clones are responsible for the cases of bovine mastitis on the various farms.Key words: S. aureus, mastitis, phenotyping, genotyping.

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Prevalence and etiology of buffalo mastitis and milk somatic cell count in dry and rainy seasons in a buffalo herd from Analândia, São Paulo State, Brazil

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-7259 ◽

2014 ◽

Vol 66 (6) ◽

pp. 1703-1710 ◽

Cited By ~ 5

Author(s):

L.J.L. Pizauro ◽

D.G. Silva ◽

A.M. Santana ◽

V. Clemente ◽

G.H.B. Lara ◽

...

Keyword(s):

Streptococcus Agalactiae ◽

Somatic Cell Count ◽

Sao Paulo ◽

São Paulo ◽

Dry Period ◽

Rainy Period ◽

Paulo State ◽

Milk Samples ◽

São Paulo State ◽

Dry And Rainy Seasons

The aim of this study was to evaluate somatic cell count (SCC), prevalence and etiology of mastitis in a dairy buffalo herd from Analândia, São Paulo State, Brazil, in the dry and rainy seasons. Additionally, antimicrobial susceptibility profile of microorganisms isolated from milk samples was also evaluated. 1,042 milk samples from female Murrah buffaloes in a dairy farm located in Analândia, São Paulo State, Brazil, collected between May 2011 and November 2012 were analyzed. After the mammary gland physical examination, strip cup test and California Mastitis Test (CMT) were performed. Afterwards, 50mL of milk samples from each mammary quarter were collected aseptically for SCC in automatic equipment and microbiological examination. The antimicrobial sensitivity profile to ampicillin, cefoperazone, ceftiofur, enrofloxacin, gentamicin, neomycin, oxacillin, penicillin, and sulfamethoxazole/trimethoprim was evaluated by disk diffusion method. The monthly average temperature and pluviometric index were obtained from "Centro Integrado de Informações Agrometeorológicas" (CIIAGRO) of "Instituto Agronômico de Campinas" (IAC). Milk samples with positive results in the microbiological test showed average SCC of 137,720 cells/mL in the dry period and 190,309 cells/mL in the rainy period. Although a higher number of isolated microorganisms was observed in buffalo milk samples during the rainy period (69/600) compared to the dry period (50/442), the season had no significant effect on the frequency of isolation of microorganisms. The main genera of microorganisms isolated were coagulase-negative Staphylococcus (38.4%), Streptococcus agalactiae(28.8%), and Bacillus spp. (7.56%) during the dry season and Corynebacterium sp. (23.5%), Streptococcus spp. (32.3%), and Streptococcus agalactiae (9.24%) during the rainy period. Multidrug resistance was observed in 30.1% of the isolated microorganisms.

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