scholarly journals Metabolic response of blood vessels to TNFα

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Abidemi Junaid ◽  
Johannes Schoeman ◽  
Wei Yang ◽  
Wendy Stam ◽  
Alireza Mashaghi ◽  
...  

TNFα signaling in the vascular endothelium elicits multiple inflammatory responses that drive vascular destabilization and leakage. Bioactive lipids are main drivers of these processes. In vitro mechanistic studies of bioactive lipids have been largely based on two-dimensional endothelial cell cultures that, due to lack of laminar flow and the growth of the cells on non-compliant stiff substrates, often display a pro-inflammatory phenotype. This complicates the assessment of inflammatory processes. Three-dimensional microvessels-on-a-chip models provide a unique opportunity to generate endothelial microvessels in a more physiological environment. Using an optimized targeted liquid chromatography–tandem mass spectrometry measurements of a panel of pro- and anti-inflammatory bioactive lipids, we measure the profile changes upon administration of TNFα. We demonstrate that bioactive lipid profiles can be readily detected from three-dimensional microvessels-on-a-chip and display a more dynamic, less inflammatory response to TNFα, that resembles more the human situation, compared to classical two-dimensional endothelial cell cultures.

1975 ◽  
Author(s):  
Payling H. Wright ◽  
M. Evans

Cultures of vascular endothelium obtained from fresh human umbilical veins and grown in vitro in fortified 199 medium for several days have been subjected to differing concentrations of adrenalin for various times. Their reactions to the drug, as seen microscopically, were recorded photographically. The viability of endothelial cells under these cultural conditions gives a measure of the maximal exposure to adrenalin which they are able not only to survive, but also to multiply. Their capacity to mitose was studied autor adiographically.The significance of the findings will be discussed with reference to atherogenesis and particularly the possible link with infection and in “stress”.


Metabolism ◽  
1994 ◽  
Vol 43 (7) ◽  
pp. 878-882 ◽  
Author(s):  
Kaj Metsärinne ◽  
Outi Saijonmaa ◽  
Hannele Yki-Järvinen ◽  
Frej Fyhrquist

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