scholarly journals Semibarbula orientalis (Web.) Wijk. and Marg: A Potential Source of Bioactive and High Value Phytochemicals

Author(s):  
Vinod Deora ◽  
G. S. Deora

Aims: Present study aims to evaluate the presence of bioactive compounds of Semibarbula orientalis (Pottiales: Pottiaceae), a bryophyte whole plant methanolic extract by Gas Chromatography-Mass Spectrometry (GC-MS) which are important medicinally as well flavouring and colouring agents. Study design: Qualitative preliminary phytochemical and GC-MS analysis. Place and duration of study: The study was carried out at Department of Botany, Center of Advanced Study, Jai Narain Vyas University, Jodhpur-Rajasthan (India) from January 2017 to December 2020. Methodology: The Preliminary phytochemical screening of S.orientalis was carried out qualitatively following the standard methods of Harbourne, Trease and Evans. GC-MS analysis was performed by GC-MS-QP 2010 Shimadzu, Japan equipped with thermal desorption system TD 20. Results: Preliminary phytochemical analysis revealed the presence of carbohydrates, proteins, phenols, sterols, flavonoids and terpenoids. GC-MS analysis of methanolic extract of whole plant revealed the presence of 49 bioactive phytoconstituents which include mainly n-Hexadecanoic acid, Cis-vaccenic acid, Azulene, Hexadecanoic acid metyl ester, 1,3-Propanediol, 2-(hydroxyl methyl)-2-nitro, 9-12 Octadecadienol chloride (z,z), Octadecanoic acid etc. Conclusion: Preliminary phytochemical and GC-MS profiling of methanolic extract Semibarbula orientalis whole plant showed the presence of high value bioactive compounds with important medicinal properties and other uses in food industries as flavouring and colouring agents.

2019 ◽  
Vol 9 (1) ◽  
pp. 90-94
Author(s):  
P Sasikala ◽  
S Ganesan ◽  
T Jayaseelan ◽  
S Azhagumadhavan ◽  
M Padma ◽  
...  

Objective: To identify the phytochemical constituents present in ethanolic leaves extract of Taraxacum officinale (T. o) and to elucidate the bioactive compounds. Methods: The fresh leaves of T. officinale (1000g) were shade dried at room temperature for 30 days and the dried leaves were made into a fine powder. The ethanolic leaves extract obtained was dried with help of desiccator and rotatory evaporator. The dried samples tested for phytochemical analysis and bioactive compounds profiling through GC-MS analysis. Results: The phytochemical screening studies showed presence of alkaloids, flavonoids, phenol, cardiac glycosides and anthraquinones. in the leaves extract of T. officinale. GC-MS analysis showed 10 essential bioactive compounds and it revealed the presence of significant anticancer, Antimicrobial  and antidiabetic compounds. Conclusions: The ethanol extract of T. o possess the most important phytochemical compounds and it has various  medicinal  properties to cure dangerous diseases and disorders. Keywords: Taraxacum officinale, Phytochemicals analysis, GC–MS analysis, Biological compounds.


Author(s):  
I. I. Asoro ◽  
O. A. T. Ebuehi ◽  
M. N. Igwo-Ezikpe

Bioactive compounds are the frontline potent agents in both nutraceuticals and pharmaceutical industries. The bioactive compounds are gaining much importance for their ability in enhancing resistance to various diseases and to improve the health of people both by traditional and modern ways of administrations. R. vomitoria is one of the medicinal plants used traditionally to manage hypertension, diabetes and mental disorder. This present study sought to characterize the bioactive components of R. vomitoria leaf and root ethanol extracts using Gas-Chromatography-Mass Spectrophotometry (GC-MS). The results of the GC-MS analysis provide different peaks indicating the presence of 22 phytochemicals in the plant leaf and 16 phytochemicals in the root. The major bioactive compounds in the leaf were squalene (18.69%), phytol (16.47%), n-hexadecanoic acid (15.68%), 7-tetradecenal, (Z) (12.90%), 9,12,15-octadecatrienoic acid, ethyl ester, (Z,Z)-, (9.56%) and others, while the roots contains; cis-vaccenic acid (32.13%), n-hexadecanoic acid (15.41%), (E)-9-octadecenoic acid ethyl ester (9.83), cyclohexanecarbonitrile 1-(-4- chlorophenyl (9.45%), 8H-azeceno[5,4-b] indol-8-one, 5-ethylidene (7.66%) and other minor compounds. Pharmacological activities of these compounds indicated that the compounds present in the leaf of the plant can be used as a crude drug which could be developed into a novel drug. Some of these compounds have antimicrobial, antioxidant, hepatoprotective, hypocholesterolemic as well as cancer preventive activities amongst others. The findings suggest that there is an indication that both R. vomitoria leaves and roots contain potent bioactive compounds that may be linked to its beneficial effects on health, with the leaf taking the lead. It is therefore recommended as a plant of phytopharmaceutical significance.


2021 ◽  
Vol 16 (10) ◽  
pp. 102-110
Author(s):  
Vivek Chauhan ◽  
Sunita Devi ◽  
Sonaxi Sharma ◽  
Shamsher S. Kanwar

Carum carvi (Caraway) is a member of Apiaceae family which originated in Asia. Due to its economic importance, it is widely cultivated. Caraway is the only annual species, commonly present in arable land, moist meadows and on low lands to mountains. Caraway has different applications in pharmaceutical and food industries. Phytochemical screening of different medicinal plants is helpful in identifying new sources of industrially and therapeutically important compounds. In this study, Caraway obtained from forest surrounding the village Goherman, Lahaul and Spiti, Himachal Pradesh (India) was used. Seed extract of caraway extracted in methanol (MSE) and distilled water (WSE) was used for phytochemical analysis to determine the constituents of caraway seeds. Further, caraway seeds extract was checked for antibacterial activity [Staphylococcus aureus (ATCC 6538), Salmonella typhimurium (NCTC 74), Escherichia coli (ATCC 25922) and Staphylococcus epidermidis (ATCC 12228)], antioxidant activity and acid neutralizing activity. After analysing the results, it can be said that caraway seeds possess many medicinal properties and it can be used in enhancing human health.


Author(s):  
Yogeshwari C ◽  
Kumudha P

 Objective:The objective of this study is to characterize the phytoconstituents of Tiliacora racemosa Colebr. using gas chromatography mass spectrometry (GC-MS).Methods: Preliminary phytochemical and physicochemical analysis was carried out using standard procedures. GC-MS analysis of methanolic extract was carried out using Thermo GC-Trace Ultra version: 5.0, Thermo MS DSQ with a DB 35MS capillary standard non-polar column and gas chromatograph interfaced to a mass selective detector (MS DSQ II) with Xcalibur software.Results: Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, phenols, tannins, triterpenoids, steroids, proteins and amino acids, carbohydrates, saponins and coumarin. Quinones, anthraquinones, glycosides and fixed oil were absent. GC-MS analysis revealed the presence of 28 compounds of which quinic acid (retention times [RT]: 15.65) and inositol, 1-deoxy-(CAS) (RT: 19.24) was observed as abundant compounds.Conclusion: The presence of various bioactive compounds confirms the medicinal importance and it’s application for curing various diseases by traditional practitioners. However, isolation and characterization of potential bioactive compounds would lead to drug formulation.


2021 ◽  
Vol 72 (1) ◽  
pp. 97-108
Author(s):  
Rafeef K. Massadeh ◽  
Tamam El-Elimat ◽  
Mohammad Al-Gharaibeh ◽  
Khaled Tawaha ◽  
Feras Q. Alali

Abstract The alkaloid-rich fraction obtained by fractionation of the crude methanolic extract of the leaves of wild tobacco tree Nicotiana glauca Graham (Solanaceae) was analyzed using UPLC-MS and GC-MS. Anabasine, a piperidine alkaloid, was identified as the major constituent with approximately 60 % (m/m) of the alkaloid-rich fraction. In addition to anabasine, six secondary metabolites were identified using high-resolution UPLC-MS. Anabasine was quantified in the leaves to be 1 mg g−1 dry plant material. The GC-MS analysis revealed five compounds with anabasine as the major component, while nicotine was not detected. Moreover, GC-MS was used for the analysis of the volatile oil that was obtained by hydro-distillation from the leaves of N. glauca. The volatile plant oil was found to be rich in oxygenated sesquiterpenes (e.g., β-bisabolol) and carboxylic acids and esters (e.g., ethyl linoleate and hexadecanoic acid), whereas anabasine was not detected.


2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Mariel Monrroy ◽  
Onix Araúz ◽  
José Renán García

Nephelium lappaceum and its by-products have great potential in the agricultural, pharmaceutical, and food industries. Some studies have shown that N. lappaceum by-products exhibit antimicrobial, antioxidant, antidiabetic, and anticancer properties. However, studies focused on identifying these compounds are rare. The availability of polyphenolic compounds can vary according to environmental conditions, soil, plant variety, and agronomic management. Therefore, in this study, the active compounds in extracts of the N. lappaceum peel were identified, and their antioxidant properties were evaluated using various extraction solvents and both ultrasonic and boiling extraction techniques. The chemical characterization of the N. lappaceum peel exhibited carbohydrate and reducing sugar contents of 12 and 2%, respectively. Phytochemical analysis indicated the presence of flavonoids, tannins, terpenes, and steroids. The total phenolic and flavonoid contents and total antioxidant capacity were the highest in the hydroethanolic extract obtained by ultrasound, with values of 340 mg gallic acid equivalents g−1, 76 mg quercetin equivalents g−1, and 2.9 mmol of Trolox equivalents g−1, respectively. Contrarily, the total anthocyanin content was higher in the acid extract obtained by ultrasound, with a value of 0.7 mg cyanidin-3-O-glucoside equivalents g−1. A total of 18 compounds—including hydroxybenzene, phenolic acid, flavonoids, fatty acids (saturated, unsaturated, and ester), vitamin, arenecarbaldehyde, and phthalate—were identified for the first time in the N. lappaceum peel using gas chromatography-mass spectrometry. The identified compounds have been previously isolated from other plants and reportedly exhibit anticancer, anti-inflammatory, antimicrobial, and antioxidant activities. Thus, the N. lappaceum peel was shown to be a potential source of bioactive compounds of immense importance in the pharmacological and food industries.


Author(s):  
Kuladip Gurav ◽  
Varsha Jadhav (rathod)

Objective: The aim was to investigate important bioactive compounds, biological activities, and medicinal importance of Zanthoxylum rhetsa fruits.Methods: The present work was carried out by gas chromatography–mass spectrometry (GC-MS) method for the identification of different compounds.Result: The methanolic extract of fruits showed 32 chemical compounds which are identified through GC-MS analysis. Among them, some of the compound names and percentage values are as follows: 2-propanone, 1,3-dihydroxy (48.9%), 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl (33.7%), 2-furancarboxaldehyde, 5-[hydroxymethyl] (50.2%), 1-Heptatriacontanol (34.4%), 9,12-octadecadienoic acid (zz)- (48.6%), cholestan- 3-ol,2-methylene, [3β,5α] (75.0%), 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl (90.8), 2-furancarboxaldehyde, 5-[hydroxymethyl] (92.0%), hydroquinone (64.9%), n-hexadecanoic acid (37.5%), octadecanoic acid (28.2%), 9,12,15, octadecatrienoic acid, 2-[(trimethylsiyl]oxy]-1- [(trimethylsiyl]oxy]methyl] ethyl ester, [zzz] (22.6%), 9-hexadecanoic acid (10.3%), digitoxin (18.8%), 8,11,14-eicosatrienoic acid methyl ester, [zzz] (25.5%), and oleic acid (16.5%). Most of the identified compounds in the crude methanolic extracts exhibit some bioactivities, namely anticancer, anti-inflammatory, antimicrobial, hepatoprotective, antioxidant, hypocholesterolemic, nematicide, pesticide, anti-androgenic flavor, hemolytic, 5-alpha-reductase inhibitor, insectifuge, antiarthritic, anti-coronary, cardiovascular, anti-breast cancer, aromatic, and insectifuge. On the basis of the above investigation, the fruits can be recommended as a treasure of bioactive compounds and it plays a promising role in herbal medicine.Conclusion: The present study reveals that fruits of Z. rhetsa contain various bioactive compounds. Digitoxin is recorded in the ripened fruit of Z. rhetsa and it shows the anticancerous and cardiac arrest properties. Hence, in future, this plant will play a promising role in curing cancer.


Author(s):  
ARCHANA ELAMKULAM RAVINDRAN ◽  
JOHN ERNEST THOPPIL

Objective: The present study aims to analyze the potential of Aglaia edulis Roxb. leaf extract to induce cytological aberrations in Allium cepa root meristem and to determine the phytoconstituents in the extract. Methods: Cytotoxicity evaluation of the leaf methanolic extract was done using Allium cepa assay using various concentrations. Volatile phytoconstituents in the extract were determined using gas chromatography–mass spectrometry analysis. Results: Considerable number of cytomictic cells along with other aberrations was observed. The occurrence of cytomixis was found to be dose dependent where it ranged from 6.58±0.35 to 29.45±0.45. The percentage of cytomictic cells among the total aberrant cells was observed between 35.19±1.67 and 77.39±1.39. The phytochemical analysis of the plant extract revealed the presence of active secondary metabolites. Conclusion: The synergistic action of the active compounds might have triggered the phenomenon of cytomixis which, in turn, could be exploited for the production of polyploids.


Plants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 646 ◽  
Author(s):  
Majid Sharifi-Rad ◽  
Francesco Epifano ◽  
Serena Fiorito ◽  
José M. Álvarez-Suarez

This study was carried out to screen the amount and the classes of secondary metabolites and to evaluate the antioxidant, cytotoxic, antifungal, and antibacterial activities of the methanolic, ethanolic, and water extracts of the roots, leaves, and flowers of Nepeta juncea Benth. The results show that the highest total phenol (69.54 ± 0.31 mg gallic acid equivalents (GAE)/g dry weight), total flavonoid (41.37 ± 0.17 mg quercetin equivalents (QE)/g dry weight), anthocyanin (6.52 ± 0.21 mg cyanidin/100 g dry weight), and tannin (47.36 ± 0.33 mg catechin/g dry weight) concentrations were recorded in the methanolic extract of the leaves of N. juncea. The gas chromatography–mass spectrometry (GC–MS) analysis of the extracts showed that 1,8-cineole, 4aα-7α-7aα-nepetalactone, β-pinene, terpinen-4-ol, and α-terpineol were the major compounds, respectively. The best 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ferric-reducing antioxidant, cytotoxic, antifungal, and antibacterial activities were observed for the methanolic extract of the leaves. For the two latter activities, the best activity was revealed on Staphylococcus aureus, Bacillus cereus, and Candida albicans. The minimum inhibitory concentration (MIC) values for the antimicrobial of the methanolic extract from the leaves were in the range of 25–100 µg/mL, whereas the minimum bactericidal concentration (MBC) values were in the range of 50–200 µg/mL. The results reported herein show that, for the first time in the literature, N. juncea is a remarkable source of antioxidant, antifungal, and antibacterial compounds.


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