Wuweizi [Schisandra chinensis（Turcz.）Baill.] is used for traditional medicine in northeastern China. In August of 2019, root rot of S. chinensis with an incidence of 30%-50% was observed in a commercial field located in Liaozhong city (41º29’57” N, 122º52’33” E) in the Liaoning province of China. The diseased plants were less vigorous, stunted, and had leaves that turned yellow to brown. Eventually, the whole plant wilted and died. The diseased roots were poorly developed with brown lesion and eventually they would rot. To determine the causal agent, symptomatic roots were collected, small pieces of root with typical lesions were surface sterilized in 2% NaOCl for 3 min, rinsed three times in distilled water, and then plated onto PDA medium. After incubation at 26°C for 5 days, whitish-pink or carmine to rose red colonies on PDA were transferred to carnation leaf agar (CLA). Single spores were isolated with an inoculation needle using a stereomicroscope. Five single conidia isolates obtained from the colonies were incubated at 26°C for 7 days, abundant macroconidia were formed in sporodochia. Macroconidia were falcate, slender, with a distinct curve to the latter half of the apical cell, mostly 3 to 5 septate, measuring 31.3 to 47.8 × 4.8 to 7.5µm (n=50). Microconidia were oval and irregular ovals, 0-1 septate, measuring 5.0 to 17.5 × 2.5 to 17.5µm (n=50). Chlamydospores formed in chains on within or on top of the mycelium. Morphological characteristics of the isolates were in agreement with Fusarium acuminatum (Leslie and Summerell, 2006). To confirm the identity, the partial sequence of the translation elongation factor 1 alpha (TEF1-á) gene of five isolates was amplified using the primers EF-1(ATGGGTAAGGARGACAAG) and EF-2 (GGARGTACCAGTSATCATGTT) (O’Donnell et al. 2015 ) and sequenced. The rDNA internal transcribed spacer (ITS) region for the five isolates was also amplified using the primers ITS1 (TCCGTAGGTGAACCTGCGG) and ITS4 (TCCTCCGCTATTGATATGC) (White et al.1990) and sequenced. The identical sequences were obtained, and one representative sequence of isolate WW31-5 was submitted to GenBank. BLASTn analysis of the TEF-á sequence (MW423624) and ITS sequence (MZ145386), revealed 100%（708/685bp, 563/563bp）sequence identity to F. acuminatum MH595498 and MW560481, respectively. Pathogenicity tests were conducted in greenhouse. Inoculums of F. acuminatum was prepared from the culture of WW31-5 incubated in 2% mung beans juice on a shaker (140 rpm) at 26°C for 5 days. Ten roots of 2-years old plants of S. chinensis were immersed in the conidial suspension (2 × 105 conidia/ml) for 6 hours, and another ten roots immersed in sterilized distilled water in plastic bucket for 6 hours. All these plants were planted into pots with sterilized field soil (two plants per pot). Five pots planted with inoculated plants and another five pots planted with uninoculated plants served as controls. All ten pots were maintained in a greenhouse at 22-26°C for 21 days and irrigated with sterilized water. The leaves of the inoculated plants became yellow，gradually dried up, eventually finally all the aboveground parts died. The roots of the inoculated plants were rotted. Non-inoculated control plants had no symptoms. F. acuminatum was reisolated from the roots of inoculated plants and had morphology identical to the original isolate. The experiment was repeated twice with similar results. F. acuminatum has been reported as a pathogen caused root rot of ginseng (Wang et al. 2016) and not reported on Wuweizi in China. To our knowledge, this is the first report of root rot of S. chinensis caused by F. acuminatum. We have also observed the disease at Benxi city of Liaoning Province in 2020 and it has become an important disease in production of S. chinensis and the effective control method should be adopted to reduce losses.
New glycosides from the leaves and rattan stems of Schisandra chinensis