Mycorrhizal Switching and the Role of Fungal Abundance in Seed Germination in a Fully Mycoheterotrophic Orchid, Gastrodia confusoides

Mycorrhizal associations are essential for orchid germination and seedling establishment, and thus may constrain the distribution and abundance of orchids under natural conditions. Previous studies have shown that germination and seedling establishment in several orchids often decline with increasing distance from adult plants, resulting in non-random spatial patterns of seedling establishment. In contrast, individuals of the fully mycoheterotrophic orchid Gastrodia confusoides often tend to have random aboveground spatial patterns of distribution within bamboo forests. Since G. confusoides is parasitic on litter-decaying fungi, its random spatial patterns of distribution may be due to highly scattered patterns of litter-decaying fungi within bamboo forests. To test this hypothesis, we first identified the main mycorrhizal fungi associating with developing seeds and adult plants at a bamboo forest site in Taiwan using Miseq high-throughput DNA sequencing. Next, we combined seed germination experiments with quantitative PCR (qPCR) analyses to investigate to what extent the abundance of mycorrhizal fungi affected spatial patterns of seed germination. Our results show that seed germination and subsequent growth to an adult stage in G. confusoides required a distinct switch in mycorrhizal partners, in which protocorms associated with a single Mycena OTU, while adults mainly associated with an OTU from the genus Gymnopus. A strong, positive relationship was observed between germination and Mycena abundance in the litter, but not between germination and Gymnopus abundance. Fungal abundance was not significantly related to the distance from the adult plants, and consequently germination was also not significantly related to the distance from adult plants. Our results provide the first evidence that the abundance of litter-decaying fungi varies randomly within the bamboo forest and independently from G. confusoides adults.

Strip Clearcutting Drives Vegetation Diversity and Composition in the Moso Bamboo Forests

Strip clearcutting of Moso bamboo forests in southern China has seen increasing interest as a way of reducing harvesting costs. Previous research has shown that cutting influences the overstory structure and drives changes in the microclimate and soil properties. However, the effects of strip cutting on understory vegetation diversity and composition remain unclear. To better understand the influence of cutting on the understory vegetation, this study compares sites under natural restoration after cut and uncut sites in the Moso bamboo forest. We selected plots that were cut in 2019 (C19) and 2017 (C17), as well as unharvested plots as controls (CK). The results showed that strip clearcutting increased the understory vegetation richness and diversity, and a significant difference (A = 0.23, P = 0.001) existed in the composition of the vegetation between the three treatments. Furthermore, the decrease of soil total phosphorus and total potassium content resulted in the difference in undergrowth vegetation distribution and composition between the uncut plots and the cut plots. Our results suggest that strip clearcutting may not be harmful to biodiversity on a local scale in the Moso bamboo forest. Study Implications: This study demonstrates that strip clearcutting, which is an economically important harvesting method for bamboo, had significant effects on understory vegetation composition and diversity, and understory vegetation has not returned to preharvest levels after two years. The understory vegetation was affected by soil nutrient content and light conditions in the forest. We believe our research has made a significant contribution to the literature because bamboo is commercially important and its sustainable management is needed by many industries. This study highlights the impact of strip cutting on understory vegetation. The retention of understory vegetation characteristics is critical for the sustainable management of these forests, and this study not only demonstrates the dynamics of cut plots recovery but also increases our knowledge of this important species.

Evaluation of Nutritional Ingredients, Biologically Active Materials, and Pharmacological Activities of Stropharia rugosoannulata Grown under the Bamboo Forest and in the Greenhouse

Evaluations of the nutritional ingredients, biologically active materials, and pharmacological activities (antibacterial and antioxidant) of two types of Stropharia rugosoannulata, grown under the bamboo forest and in the greenhouse, respectively, were carried out in this study. The nutritional ingredient tests included moisture, ash, crude protein, crude fat, crude fiber, total carbohydrates, total sugar, mineral elements, vitamins, and amino acids. The biologically active material assays included total flavonoids, total polysaccharides, total triterpenoids, and reducing sugars. In addition, a broth microdilution susceptibility test was performed to evaluate antibacterial activity. The antioxidant activity was measured by ABTS+ radical scavenging, ferric-reducing power, and β-carotene bleaching assays. Results showed that two types of S. rugosoannulata were rich in protein, carbohydrate, fiber, mineral, B-group vitamins, polysaccharides, triterpenoids, and reducing sugars, with low content of fat and low Na/K ratio. Besides, they were effective in antibacterial and antioxidant activities, while S. Rugosoannulata grown under the bamboo forest was better than the greenhouse one in some respects such as higher content of protein, calcium, selenium, nicotinic acid, folic acid, polysaccharides and triterpenoids, and stronger antibacterial and antioxidant activities. They were appropriate for functional food and nutritional supplements, especially bamboo forest.

Forest Bathing: A New Attraction and Disaster Mitigation for Batur UNESCO Global Geopark Bali

Forests have ecological and sociological functions, including tourism development as well as disaster mitigation efforts. In the conditions of the Covid-19 pandemic that hit Bali tourism, it is necessary to develop new types of tourism such as this forest bathing. This research intends to study the potential and opportunities of bamboo forests in the Batur Geopark area to be used as new types of tourism, which at the same time can also be used as a disaster mitigation. A case study method with qualitative data collection includes observation, in-depth interviews, and literature study. A mapping plan for the bamboo forest area is also carried out to support the facilities and infrastructure for forest bathing activities. Bamboo has been used for the restoration of areas affected by the fire at Geopark Batur. However, no one has specifically offered the concept of Forest Bathing. Introducing forest bathing as a new attraction to revive Bali tourism and at the same time to preserve the environment is possible and necessary. Furthermore, there is important to develop destination branding and marketing for the forest bathing attraction.