The Modulation of Water, Nitrogen, and Phosphorous Supply for Growth Optimization of the Evergreen Shrubs Ammopiptanthus mongolicus for Revegetation Purpose

Surface mining is a critical anthropogenic activity that significantly alters the ecosystem. Revegetation practices are largely utilized to compensate for these detrimental impacts of surface mining. In this study, we investigated the effects of five water (W) regimes [W40: 40%, W48: 48%, W60: 60%, W72: 72%, and W80: 80% of field capacity (FC)], five nitrogen (N) (N0: 0, N24: 24, N60: 60, N96: 96, and N120: 120 mg kg−1 soil), and five phosphorus (P) fertilizer doses (P0: 0, P36: 36, P90: 90, P144: 144, and P180: 180 mg kg−1 soil) on morpho-physiological and biochemical parameters of Ammopiptanthus mongolicus plants to assess the capability of this species to be used for restoration purposes. The results showed that under low W-N resources, A. mongolicus exhibited poor growth performance (i.e., reduced plant height, stem diameter, and dry biomass) in coal-degraded spoils, indicating that A. mongolicus exhibited successful adaptive mechanisms by reducing its biomass production to survive long in environmental stress conditions. Compared with control, moderate to high W and N-P application rates greatly enhanced the net photosynthesis rates, transpiration rates, water-use efficiency, chlorophyll (Chl) a, Chl b, total Chl, and carotenoid contents. Under low-W content, the N-P fertilization enhanced the contents of proline and soluble sugar, as well as the activities of superoxide dismutase, catalase, and peroxidase in leaf tissues, reducing the oxidative stress. Changes in plant growth and metabolism in W-shortage conditions supplied with N-P fertilization may be an adaptive strategy that is essential for its conservation and restoration in the desert ecosystem. The best growth performance was observed in plants under W supplements corresponding to 70% of FC and N and P doses of 33 and 36 mg kg−1 soil, respectively. Our results provide useful information for revegetation and ecological restoration in coal-degraded and arid-degraded lands in the world using endangered species A. mongolicus.

Selection and Evaluation of Suitable Tree Species in Drought and High-dust Coal Base in Northwest China

To select drought-resistant and dust-tolerant tree species suitable for being planted in a drought-ridden and high-dust coal base in northwest China, we used nine tree species to determine the growth status, biomass, harm index, and photosynthetic characteristics under drought and highly dusty stress conditions. The results showed that in the drought resistance index system, their cumulative contribution rate reached 98.1%, and the drought resistance order was Ammopiptanthus mongolicus > Hedysarum scoparium > Sabina vulgaris >Caragana korshinskii > Ulmus pumila > Amorpha fruticosa > Caryopteris mongholica > Tamarix chinensis > Elaeagnus angustifolia. In the dust resistance index system, the cumulative contribution rate reached 95.2%. The dust resistance order was Caragana korshinskii > Amorpha fruticosa > Sabina vulgaris > Hedysarum scoparium > Tamarix chinensis > Ammopiptanthus mongolicus > Ulmus pumila > Caryopteris mongholica > Elaeagnus angustifolia. In the drought and dust resistance comprehensive index system, 14 indices (shoot length, stomatal conductance, and POD) had larger weights and the cumulative contribution of comprehensive indices reached 100%. The drought and dust resistance order of the test tree species was Caragana korshinskii > Ulmus pumila > Amorpha fruticosa > Sabina vulgaris > Caryopteris mongholica > Ammopiptanthus mongolicus > Hedysarum scoparium > Tamarix chinensis > Elaeagnus angustifolia. These results provided a reference for the selection of suitable greening tree species in accordance with local conditions in drought-mining areas.

Water use strategy of Ammopiptanthus mongolicus community in a drought year on the Mongolian Plateau

Aims In desert ecosystems, water is a restricting factor for plant growth and vegetation dynamics. The relatively stable water source from deep soil profile or groundwater is important for plant survival during drought. Understanding water use strategy of endangered species, in desert ecosystem is essential for their conservation and restoration such as Ammopiptanthus mongolicus on the Mongolian Plateau. Methods The stable isotope method of δD and δ 18O was used to examine the main water sources of A. mongolicus and two companion shrubs, e.g. Artemisia ordosica and Artemisia xerophytica. The contribution of different water sources to each species was calculated by IsoSource model. Leaf δ 13C was used to compare long-term water use efficiency of three shrubs. Soil moisture and root distribution of three shrubs was measured to explain plant water use strategy. Important Findings The results showed that A. mongolicus relied on groundwater and 150–200 cm deep soil water, with the former contributing to almost half of its total water source. Artemisia ordosica mainly used 150–200 cm deep soil water, but also used shallow soil water within 100 cm in summer and autumn. Artemisia xerophytica mainly used 150–200 cm deep soil water and groundwater, with the latter contributing to about 30%–60% of its total water source. The three shrubs had dimorphic or deep root systems, which are in accord with their water sources. The WUE in the evergreen shrub A. mongolicus was higher than in two deciduous Artemisia shrubs, which may be an adaptive advantage in desert ecosystem. Therefore, groundwater is an important water source for the endangered shrub A. mongolicus in a drought year on Mongolian Plateau. Ammopiptanthus mongolicus and two Artemisia shrubs competed for deep soil water and groundwater.

Heterologous Expression of Three Ammopiptanthus mongolicus Dehydrin Genes Confers Abiotic Stress Tolerance in Arabidopsis thaliana

Ammopiptanthus mongolicus, a xerophyte plant that belongs to the family Leguminosae, adapts to extremely arid, hot, and cold environments, making it an excellent woody plant to study the molecular mechanisms underlying abiotic stress tolerance. Three dehydrin genes, AmDHN132, AmDHN154, and AmDHN200 were cloned from abiotic stress treated A. mongolicus seedlings. Cytomembrane-located AmDHN200, nucleus-located AmDHN154, and cytoplasm and nucleus-located AmDHN132 were characterized by constitutive overexpression of their genes in Arabidopsis thaliana. Overexpression of AmDHN132, AmDHN154, and AmDHN200 in transgenic Arabidopsis improved salt, osmotic, and cold tolerances, with AmDHN132 having the largest effect, whereas the growth of transformed plants is not negatively affected. These results indicate that AmDHNs contribute to the abiotic stress tolerance of A. mongolicus and that AmDHN genes function differently in response to abiotic stresses. Furthermore, they have the potential to be used in the genetic engineering of stress tolerance in higher plants.