Increasing nitrogen fertilizer use efficiency has become an environmental and economic demand in order to minimize losses of nitrogen and maximize the output from nitrogen added. The application of organic amendments with N fertilizers could be proposed as an important economic and environmental practice for improving N fertilizer use. A two-year field experiment was carried out using the 15N tracer technique to study the impact of corn straw and woody peat application on uptake and utilization of N fertilizer by maize plant. Three treatments were set up: CK (15N labeled urea alone), CS (15N labeled urea + crushed corn straw) and WP (15N labeled urea+ crushed woody peat). The results showed that, as compared to CK, both straw and peat treatments led to (i) an increase in yield of maize, 15N urea utilization rate, and residual 15N urea remained in soil by 11.20% and 19.47%, 18.62% and 58.99%, 41.77% and 59.45%, respectively, but (ii) a decrease in the total loss rate by 6.21% and 16.83% (p < 0.05), respectively over the two seasons. Moreover, the significantly highest effect was recorded with woody peat application rather than that with corn straw. Our study suggests that corn straw and woody peat can be used as organic fertilizers to increase maize yields, promote nitrogen fertilizer balance sheet, reduce the leaching of N fertilizer into the subsurface soil layer, and facilitate the further absorption and utilization of soil residual nitrogen. Therefore, the application of humified organic material play a crucial role in N utilization efficiency enhancement.