The Protective Role of 1,8-Dihydroxynaphthalene–Melanin on Conidia of the Opportunistic Human Pathogen Aspergillus fumigatus Revisited: No Role in Protection against Hydrogen Peroxide and Superoxides

Opportunistic pathogens like Aspergillus fumigatus have strategies to protect themselves against reactive oxygen species like hydrogen peroxides and superoxides that are produced by immune cells. DHN-melanin is the green pigment on conidia of Aspergillus fumigatus and more than 2 decades ago was reported to protect conidia against hydrogen peroxide.

A Study on the Conservation and Management of the Painting of Shamanistic Spirits in Chiseonggwang Buddha

This study presents a method for conserving shamanistic spirits in Chiseonggwang Buddha. Scientific investigation has revealed that these spirits have been subject to degeneration as a result of severe exfoliation and pollution. The materials and preservation treatment techniques used in create these shamanistic spirits were identified through visual inspection and using appropriate scientific equipment. The different types of background paper, background material, and color pigments used in create the shamanistic spirits were analyzed using a colorimeter, stereoscopic microscope, and SEM-EDS techniques. The analysis revealed that the pulp paper was used as the background and synthetic fiber polyester as the background material. In addition, the study of the pigment revealed that the color components were all synthetic, except for red lead [Pb3O4] and oyster shell white [CaCO3]. Moreover, it was confirmed that the green pigment, identified as emerald green [Cu(C2H3O2)2.3Cu(AsO2)2], was a major component of shamanistic spirits in the late 19th century. The shamanistic spirits in Chiseonggwang Buddha were conserved by identifying raw materials and pigments through this detailed analysis.

«PORTRAIT OF A LADY» OF THE BOICHUCK SCHOOL FROM THE NATIONAL ACADEMY OF FINE ARTS AND ARCHITECTURE FUNDS. COMPREHENSIVE EXAMINATION RESULTS

The article features the results of the comprehensive examination of an outstanding piece from the Mykhailo Boуchuk school, «Portrait of a Lady» from the NAFAA funds. The research was done by the Associate Professor of the Department of the Technique and Restoration of Artwork of NAFAA, Tetiana Tymchenko, Ph.D, and the Leading Researcher of the Bureau of scientific and technical expertise «ART-LAB» Svitlana Biskulova, Ph.D, in connection with the planned restoration of the artwork. Since 2018, «Portrait of a Lady» has been attributed to Mykola Kasperovych (1885–1938), an outstanding painter, restorer and researcher. The reasoning is provided in the expert conclusion of the Head of the Ukrainian Art Department of the end of 19th — beginning of the 20th century of the National Art Museum of Ukraine Olena Kashuba-Volvach. The article lists the peculiarities of the techniques, technology, condition of the artwork from the NAFAA funds. In particular, it reveals the peculiarities of the preparatory drawing, imitation of the monumental art techniques by means of the easel painting, and individual techniques of working with paints. The paper specifies the time of the portrait creation, which indicates 1923 — probably, the year of the work creation, and the age of the lady from the portrait, 30 years. The technological examination of the portrait in the Bureau of scientific and technical expertise «ART-LAB» confirmed the dating of the work to the period of the 1920s – 1930s. The article clarifies important components of the portrait technology: in particular, it has been found that the ground is chalk-glue and the paints’ medium is casein-oil tempera; among the pigments the zinc white with an admixture of lead white, yellow and red ochre, Ivory black pigment (burnt bone), mixed green pigment (burnt bone and yellow ochre) were identified. The examination revealed the traces of restoration interventions of different times. The paper features a number of considerations regarding further research of Boychukists’ creativity from the point of view of technology.

The Symbolism of Green Colour in Neolithic of Serbia

The focus of this text is twofold. The first task is to reconsider the mode of interpretation of various small (4 to 10 cm) objects (axes, chisels, finger-rings, pendants), made of green minerals – jadeite and/or nephrite. These objects are registered on 15 sites in the territory of present-day Serbia, dated into the Neolithic period (6200/6000–4600/4500 cal BC). The second task is to lay out a short history of the green colour. As stated by Michel Pastoureau (Pasturo 2015), the production of a green pigment was almost impossible in the past, especially in praehistory. The first recorded attempts are linked to Ancient Egypt, and the oldest successful production of the green pigment is dated to Ancient Rome. Therefore, the text examines the symbolic role of these small green objects during the Neolithic. The beds of green minerals jadeite and nephrite are not recorded in the territory of Serbia. Jadeite may be found south of Skopje (Mt. Solunska glava), in the Cyclades in Greece, as well as in the Piedmont Alps. Nephrite is registered in the mountain Ogražden (SW Bulgaria), in Poland, Sweden, and Italy. The absence of mineral layers in the region surely suggests the existence of long-distance exchange. This raises the issue of the role and symbolic function of these objects – what was their appeal for the past communities and what was the role of their colour in their manufacture? In archaeology, the studies in symbolics of various colours are rare. Authors have mainly focused upon the origin of the colour and the dating of the objects, but the role of the colour itself as the criterion in choosing various raw materials has largely been neglected. Notable is the exception of the thematic collection “Colouring the Past: The Significance of Colour in Archaeological Research” (2002), where attempts have been made to explain the symbolic importance of various colours in the past. However, the colour green is not mentioned. This paper does not aim to offer final solutions, but to investigate the usage and importance of the colour green on the grounds of the usage of the objects themselves.

Molasses as a new nutrition medium for Scenedsmus quadricauda growth and production of some bio compounds

Algae comprise a large group of Thallophyta, which may be used as direct nutrition of human beings. Molasses is the by-product of the sugar manufacturing facility. In this study, a locally isolated Scendsmus quadricauda from the environment of Mosul in the Shalalat region was obtained. Biomass of Scenedsmus was measurement by carried out and filtration then drying in an oven for 24 h and weighed, Estimation of chlorophyll and protein and carbohydrate content of Scenedsmus. The research has proved that the best growing period for Scendsmue quadricauda is 15 days when using sugar factory waste as a carbon source, the growth reached (1.42 nm) as optical density, biomass (1525 mg /L), chlorophyll (green), pigment (18 mg /l) protein content (396 mg /l ) and carbohydrates ( 501 mg / l ). The research showed that the use of sugar factory waste as a nutritional medium for algal growth in the dark (11.5%) achieved good growth of Scendesmues quadricauda ( 0.632 nm), biomass (820 mg / L), green pigment (Chlorophyll) (18 mg /L) protein content (235 mg / L ) and carbohydrates (401 mg/L). while using phosphor (0.018%) of K2HPO4 in dark medium achieved highest growth rate (0.91 nm) , biomass (1110 mg / L) chlorophyll ( 22 mg/L) protein (301mg/L) and carbohydrate (461 mg/L) . It is noted too , that using IAA (0.5 g/L) in dark medium support best growth (0.888 nm) , biomass (1010 mg/L) chlorophyll (25 mg/L) , protein (230mg/L) and carbohydrate (440 mg//L) . The study showed that thiamine (1 g/L) in dark medium achieved highest growth (0.750 nm) biomass (218 mg/L), chlorophyll (29mg/L), protein (220 mg/L), carbohydrate (340mg/L). Therefore, using Molasses can enhance the growth, biomass, chlorophyll, protein, and carbohydrate content in the S. quadricauda.

Green Plant Pigment, Chlorophyllin, Ameliorates Non-alcoholic Fatty Liver Diseases (NAFLDs) Through Modulating Gut Microbiome in Mice

Non-alcoholic fatty liver diseases (NAFLDs) along with metabolic syndrome and Type-2 diabetes (T2D) are increasingly prevalent worldwide. Without an effective resolution, simple hepatic steatosis may lead to non-alcoholic steatohepatitis (NASH), characterized by hepatocyte damage, chronic inflammation, necrosis, fatty degeneration, and cirrhosis. The gut microbiome is vital for metabolic homeostasis. Conversely, dysbiosis contributes to metabolic diseases including NAFLD. Specifically, diet composition is critical for the enterotype of gut microbiota. We reasoned that green pigment rich in vegetables may modulate the gut microbiome for metabolic homeostasis. In this study, C57BL/6 mice under a high fat diet (HFD) were treated with sodium copper chlorophyllin (CHL), a water-soluble derivative of chlorophyll, in drinking water. After 28 weeks of HFD feeding, liver steatosis was established accompanied by gut microbiota dysbiosis, intestinal impairment, endotoxemia, systemic inflammation, and insulin resistance. Administration of CHL effectively alleviated systemic and intestinal inflammation and maintained tight junction in the intestinal barrier. CHL rebalanced gut microbiota in the mice under high fat feeding and attenuated hepatic steatosis, insulin resistance, dyslipidemia, and reduced body weight. Fecal flora transplants from the CHL-treated mice ameliorated steatosis as well. Thus, dietary green pigment or the administration of CHL may maintain gut eubiosis and intestinal integrity to attenuate systemic inflammation and relieve NASH.

Use of organic biostimulant for growing Siberian spruce seedlings

This study aims to assess the possibility of using a biostimulant Verva-spruce based on spruce’s natural phenolic compounds to reduce the time of growing planting material with improved features. The targets were seeds and 1-4-year-old seedlings of Siberian spruce, untreated and treated with the biostimulant. The effect of the biostimulant on seed germination, seedlings growth, and the pigment’s content in needles were studied. Results shown that soaking seeds in biostimulant at a concentration of 0.00025% increased the germination energy and accelerated hypocotyl growth. Moreover, using the biostimulant significantly increased the growth rate of experimental seedlings and heightened the amount of green pigment chlorophyll a up to 2.5 times. In 2020, in order to study the dynamics of the qualitative characteristics of the plants grown using biostimulant, experimental forest plantations of 4-year-old Siberian spruce seedlings were planted in the Altai-Sayan mountain taiga area. The experimental plantation will be monitored at least until the closure of the canopy.

Glucose Induces Thylakoid Formation and Upregulates Green Pigment Contents in Complete Dark Culture of the Angiosperm Pachiramacrocarpa

In addition to angiosperms, most plants are able to synthesize chlorophyll (Chl)-generating green tissues in total darkness. In this study, 140 plants of the angiosperm Pachira macrocarpa were divided into five groups. Among them, one group was grown for 2 weeks under natural light conditions, whereas the others were grown in complete darkness (0 μmol m−2 s−1). Dark-grown plants were then treated with 0~6% glucose for another 8 weeks. The budding and greening ratios, ultrastructure of chloroplasts (ChlPs) of newly developed leaves, and green pigment contents of pre-illuminated mature and young leaves, and totally dark-grown newly developed leaves were measured. Results showed that glucose inhibited the budding and promoted the greening of newly developed leaves. Pre-illuminated mature and young leaves were able to synthesize green pigments during the 2 weeks of dark adaption. Dark-grown newly developed leaves contained high levels of green pigments at 2 and 3 weeks after budding. Green pigments of glucose-fed newly developed leaves had increased, whereas they had decreased in control leaves. In addition, ChlPs of dark-grown glucose-fed newly developed leaves contained both giant grana and prolamellar bodies (PLBs), usually found in shade plants and etiolated seedlings, respectively. The higher the glucose concentration was, the greater the numbers of grana, thylakoids, and PLBs. Glucose increased the green pigment contents and grana formation in newly developed leaves in a dark condition, and the mechanisms are discussed.

Analysis of Characteristics Pigment And Paper Excavated At The Site of Hoeamsa Temple (12th-Century), Korea

One paper fragment and three pigments were excavated from earthenware found in the Hoeamsa Temple site (Historic Site No. 128), which is presumed to have been constructed in the 12th century. This study identifies the types and characteristics of the paper and pigments through methods such as fiber identification, pigment component analysis, and crystal structure analysis. Using the Graff 'C' staining method, the paper fragment is identified as paper-mulberry bast fiber (B. kazinoki Siebold), and according to radiocarbon dating results, the paper is believed to be from the Joseon Dynasty, between 1460 and 1640, which is when the Hoeamsa temple was believed to have been rebuilt. The green pigment consists of cerussite, malachite, and ataccamite, while quartz, muscovite, and illite were detected in the white pigment, and quartz, hematite, illite, and kaolinite were detected in the red pigment. The L* value ranged from 41.59 to 79.05. The white pigment showed the highest brightness value at 79.05, while the green pigment was measured at 58.20 and the red pigment at 41.59. The a* value ranged from -2.25 to 22.75: the a* value of the red pigment was the highest at 22.75, the green pigment was the lowest at -2.25. The b* value ranged from 7.44 to 26.26: the b*value of the red pigment was the highest at 26.26 and the white pigment was the lowest at 7.44.These scientific data identified the raw materials of paper used during the Joseon Dynasty. In addition, the data can be used for repair and restoration of ancient dancheong (traditional Korean decorative coloring on wooden buildings and artifacts) by analyzing the composition of pigments and identifying the characteristics of crystal structure.