The relevance of this article is due to the need to study the gender specifics of the relationship between the manifestations of subjectivity (mental states, procedural and effective parameters of the situation) with the basic psychological need for autonomy to apply the data obtained in the practice of psychological and pedagogical support of the educational process at the university. The goal of the empirical research was to determine the gender common and peculiar in the manifestations of subjectivity, depending on the degree of autonomy of the subject of activity. The main hypothesis was the assumption that there are differences in the manifestations of subjectivity in situations with different levels of autonomy among respondents of different genders. The sample consisted of 112 people (56 men and 56 women). The age of men was from 22 to 42 years (M=25, SD=3,5 years). The age of women was from 22 to 45 years (M=24, SD=2,8 years). The respondents - advanced students (n=112) in the form of a free essay described the situations of extrinsic and internal motivation when studying at the university and compared them with each other by any possible criteria. For data processing, the methods of content analysis, frequency analysis of text and the Fisher angular transformation criterion were used. Statistically significant differences in the emotional and intellectual-emotional manifestations of states in subjects of different sexes were revealed. In situations of autonomy, women more often experienced states of joy, interest and passion (p≤0,05), and men experienced pleasure (p≤0,01). The state of relief in situations of non-autonomy was more common for women than for men (p≤0,01). Differences in the frequency of mention of procedural and effective parameters of situations in respondents of different sexes were not found. The results of the study can be used in the development of programs for the adaptation of future specialists to changes in the labor market conditions.