Genome-Wide Novel Genic Microsatellite Marker Resource Development and Validation for Genetic Diversity and Population Structure Analysis of Banana

Trait tagging through molecular markers is an important molecular breeding tool for crop improvement. SSR markers encoded by functionally relevant parts of a genome are well suited for this task because they may be directly related to traits. However, a limited number of these markers are known for Musa spp. Here, we report 35136 novel functionally relevant SSR markers (FRSMs). Among these, 17,561, 15,373 and 16,286 FRSMs were mapped in-silico to the genomes of Musa acuminata, M. balbisiana and M. schizocarpa, respectively. A set of 273 markers was validated using eight accessions of Musa spp., from which 259 markers (95%) produced a PCR product of the expected size and 203 (74%) were polymorphic. In-silico comparative mapping of FRSMs onto Musa and related species indicated sequence-based orthology and synteny relationships among the chromosomes of Musa and other plant species. Fifteen FRSMs were used to estimate the phylogenetic relationships among 50 banana accessions, and the results revealed that all banana accessions group into two major clusters according to their genomic background. Here, we report the first large-scale development and characterization of functionally relevant Musa SSR markers. We demonstrate their utility for germplasm characterization, genetic diversity studies, and comparative mapping in Musa spp. and other monocot species. The sequences for these novel markers are freely available via a searchable web interface called Musa Marker Database.

Climate Engineering as a Communication Challenge: Contested Notions of Responsibility Across Expert Arenas of Science and Policy

Climate engineering (CE) is often said to bring about significant opportunities as well as risks. The pursuit of CE measures can be framed as either responsible or irresponsible, resulting in contentious and ambiguous communication. This article starts out from a notion of responsibility regarding subjects, objects, norms, and authorities. It will identify and analyze discursive patterns of responsibility across six expert arenas and provide a comparative mapping of these patterns. Better understanding controversy may help in finding common ground for designing research and policy strategies around CE. Taking on the challenges of communicating CE-related responsibilities would support CE governance.