Advances in Single-Cell Printing

Single-cell analysis is becoming an indispensable tool in modern biological and medical research. Single-cell isolation is the key step for single-cell analysis. Single-cell printing shows several distinct advantages among the single-cell isolation techniques, such as precise deposition, high encapsulation efficiency, and easy recovery. Therefore, recent developments in single-cell printing have attracted extensive attention. We review herein the recently developed bioprinting strategies with single-cell resolution, with a special focus on inkjet-like single-cell printing. First, we discuss the common cell printing strategies and introduce several typical and advanced printing strategies. Then, we introduce several typical applications based on single-cell printing, from single-cell array screening and mass spectrometry-based single-cell analysis to three-dimensional tissue formation. In the last part, we discuss the pros and cons of the single-cell strategies and provide a brief outlook for single-cell printing.

Enhancement of gain using a multilayer superstrate metasurface cell array with a microstrip patch antenna

This research presents a new idea in the use of wireless communication antennas: it uses a multi-layered array of cells called a superstrate multi-layer metasurface (MTM) and is placed in front of a patch of microstrip antenna to absorb surface waves and prevent them from passing through the insulating material, which reduces the permeability of the insulator and thus improves the Antenna properties, The proposed hexagonal cell with resonators is placed on the flame resistant (FR4) substrate, with a relative permittivity of 4.3 and an area (14×14) mm2 . It was tested when the metasurface layer is 4 mm in front of the patch and the distance between the metasurface layers is 2 mm. The optimum distances were calculated by the sweep parameter, and the improved antenna gain and the input reflection coefficient were obtained together. (S11) has been improved from -31.217 to -38.338 dB and, the gain from 3.28 dB to 6.554 dB.

A wideband and directive metasurface FPC antenna with toroidal metal structure loading

In this paper, a novel metasurface-based Fabry−Perot cavity antenna loaded with toroidal metal structures is presented. The antenna is compact, wideband, and has high directivity and a high front-to-back ratio. The idea of the antenna is based on loading a microstrip narrow band patch antenna resonating at 4.5 GHz by a single layer metasurface superstrate and with a toroidal metal structure. The metasurface superstrate comprises a periodic array of square patch cells. Compared to conventional microstrip antenna, the front to back lobe ratio is increased from 7 to 20 dB and the directivity is increased by 7 dB. Also, the antenna impedance bandwidth is 34% which is increased four times. This is the first-ever antenna with enhanced bandwidth and directivity using a single layer of metasurface and that too made up of periodic cell array and has application as an energy harvester.

Dielectrophoretic Micro-Organization of Chondrocytes to Regenerate Mechanically Anisotropic Cartilaginous Tissue

Recently, many studies have focused on the repair and regeneration of damaged articular cartilage using tissue engineering. In tissue engineering therapy, cells are cultured in vitro to create a three-dimensional (3-D) tissue designed to replace the damaged cartilage. Although tissue engineering is a useful approach to regenerating cartilage, mechanical anisotropy has not been reconstructed from a cellular organization level. This study aims to create mechanically anisotropic cartilaginous tissue using dielectrophoretic cell patterning and gel-sheet lamination. Bovine chondrocytes were patterned in a hydrogel to form line-array cell clusters via negative dielectrophoresis (DEP). The results indicate that the embedded chondrocytes remained viable and reconstructed cartilaginous tissue along the patterned cell array. Moreover, the agarose gel, in which chondrocytes were patterned, demonstrated mechanical anisotropy. In summary, our DEP cell patterning and gel-sheet lamination techniques would be useful for reconstructing mechanically anisotropic cartilage tissues.

One-transistor static random-access memory cell array comprising single-gated feedback field-effect transistors

In this study, we fabricated a 2 × 2 one-transistor static random-access memory (1T-SRAM) cell array comprising single-gated feedback field-effect transistors and examined their operation and memory characteristics. The individual 1T-SRAM cell had a retention time of over 900 s, nondestructive reading characteristics of 10,000 s, and an endurance of 108 cycles. The standby power of the individual 1T-SRAM cell was estimated to be 0.7 pW for holding the “0” state and 6 nW for holding the “1” state. For a selected cell in the 2 × 2 1T-SRAM cell array, nondestructive reading of the memory was conducted without any disturbance in the half-selected cells. This immunity to disturbances validated the reliability of the 1T-SRAM cell array.

A Novel Ultra-Low Power 8T SRAM-Based Compute-in-Memory Design for Binary Neural Networks

We propose a novel ultra-low-power, voltage-based compute-in-memory (CIM) design with a new single-ended 8T SRAM bit cell structure. Since the proposed SRAM bit cell uses a single bitline for CIM calculation with decoupled read and write operations, it supports a much higher energy efficiency. In addition, to separate read and write operations, the stack structure of the read unit minimizes leakage power consumption. Moreover, the proposed bit cell structure provides better read and write stability due to the isolated read path, write path and greater pull-up ratio. Compared to the state-of-the-art SRAM-CIM, our proposed SRAM-CIM does not require extra transistors for CIM vector-matrix multiplication. We implemented a 16 k (128 × 128) bit cell array for the computation of 128× neurons, and used 64× binary inputs (0 or 1) and 64 × 128 binary weights (−1 or +1) values for the binary neural networks (BNNs). Each row of the bit cell array corresponding to a single neuron consists of a total of 128 cells, 64× cells for dot-product and 64× replicas cells for ADC reference. Additionally, 64× replica cells consist of 32× cells for ADC reference and 32× cells for offset calibration. We used a row-by-row ADC for the quantized outputs of each neuron, which supports 1–7 bits of output for each neuron. The ADC uses the sweeping method using 32× duplicate bit cells, and the sweep cycle is set to 2N−1+1, where N is the number of output bits. The simulation is performed at room temperature (27 °C) using 45 nm technology via Synopsys Hspice, and all transistors in bitcells use the minimum size considering the area, power, and speed. The proposed SRAM-CIM has reduced power consumption for vector-matrix multiplication by 99.96% compared to the existing state-of-the-art SRAM-CIM. Furthermore, because of the decoupled reading unit from an internal node of latch, there is no feedback from the reading unit, with read static noise, and margin-free results.

A Negative Index Nonagonal CSRR Metamaterial-Based Compact Flexible Planar Monopole Antenna for Ultrawideband Applications Using Viscose-Wool Felt

In this paper, a compact textile ultrawideband (UWB) planar monopole antenna loaded with a metamaterial unit cell array (MTMUCA) structure with epsilon-negative (ENG) and near-zero refractive index (NZRI) properties is proposed. The proposed MTMUCA was constructed based on a combination of a rectangular- and a nonagonal-shaped unit cell. The size of the antenna was 0.825 λ0 × 0.75 λ0 × 0.075 λ0, whereas each MTMUCA was sized at 0.312 λ0 × 0.312 λ0, with respect to a free space wavelength of 7.5 GHz. The antenna was fabricated using viscose-wool felt due to its strong metal–polymer adhesion. A naturally available polymer, wool, and a human-made polymer, viscose, that was derived from regenerated cellulose fiber were used in the manufacturing of the adopted viscose-wool felt. The MTMUCA exhibits the characteristics of ENG, with a bandwidth (BW) of 11.68 GHz and an NZRI BW of 8.5 GHz. The MTMUCA was incorporated on the planar monopole to behave as a shunt LC resonator, and its working principles were described using an equivalent circuit. The results indicate a 10 dB impedance fractional bandwidth of 142% (from 2.55 to 15 GHz) in simulations, and 138.84% (from 2.63 to 14.57 GHz) in measurements obtained by the textile UWB antenna. A peak realized gain of 4.84 dBi and 4.4 dBi was achieved in simulations and measurements, respectively. A satisfactory agreement between simulations and experiments was achieved, indicating the potential of the proposed negative index metamaterial-based antenna for microwave applications.

Reduction of Whole Body Vibration in a Wide Frequency Range Using Inflation Pressure Control of Air Bladder Cushion

Several types of interfaces like foam and inflated air cells exist to reduce the effect of mechanical vibration experienced in human-machine interfaces in different scenarios such as transportation. However, their vibration attenuation performance in a wide frequency range relevant to whole body vibration (1–80 Hz) leaves much to be desired. In this study, we investigate the effect of inflation pressure on the vibration attenuation behavior of an air cell cushion. An experimental setup capable of conducting frequency sweep tests and regulating inflation pressure in an air cell array cushion was developed. Frequency sweep tests were conducted at various inflations and the vibration transmissibilities at static inflations were plotted. A dynamic inflation scheme was developed based on the apriori knowledge of inflation dependent transmissibilities. Furthermore, the closed loop behavior of the inflation scheme was evaluated with a frequency sweep test. The resulting closed loop transmissibility indicated better vibration attenuation performance than any single static inflation for the air cell array cushion in the range of frequencies relevant to whole body vibration. This result lays the groundwork for potential air cell cushions which modify their inflation dynamically through a direct feedback from sensors like accelerometers to attenuate vibration in a wide frequency range.