AbstractResearch results are often not easily accessible or readily digestible for decision-making by natural resource managers. This knowledge-action gap is due to various factors including the time lag between new knowledge generation and its transfer, lack of formal management structures, and institutional inertia to its uptake. Herein, we reflect on the Great Lakes Fishery Commission’s Science Transfer Program and its evolution from ‘Mode 1’ (i.e., scientists conduct research autonomously) toward ‘Mode 2’ (i.e., co-production of knowledge with practitioners) knowledge production to understand and overcome the knowledge-action gap. Six success factors and strategies and tactics used to achieve those factors were critical to the shift from Mode 1 to Mode 2: (1) dedicate funding and staff support; (2) obtain top-down commitment from organizational leadership; (3) break down silos; (4) build relationships through formal and informal interactions; (5) emphasize co-production in program and project implementation; and (6) obtain buy-in among relevant actors. By way of three project case studies, we highlight knowledge transfer approaches, products, and lessons learned. We anticipate this contribution will benefit those working on knowledge mobilization, particularly in boundary-spanning organizations, and those involved in resource program management, administration, and design; it is also intended for resource managers seeking to have their science and information needs met more effectively.