3D printing nanocomposite hydrogels with lattice vascular networks using stereolithography

Hydrogels have emerged as leading candidates to reproduce native extracellular matrix. To provide structures and functions similar to tissues in vivo, controlled porosity and vascular networks are required. However, fabrication techniques to introduce these are still limited. In this study we propose stereolithography as a fabrication technique to achieve 3D vascular networks using water-based solvents only. A 3D printable hydrogel is formulated based on available commercial chemicals such as acrylamide (AAm) and polyethylene glycol diacrylate 700 (PEGDA700), with nanocellulose crystals (CNC) as a nanofiller. An optimisation procedure to increase resolution, tune porosity as well as mechanical properties is developed. The results highlight the importance of photoabsorber addition to improve channel resolution. We demonstrate that with the adequate choice of chemicals and fillers for photocurable formulations, structural and functional properties of the fabricated scaffold can be tailored, opening the path for advanced applications. Graphic abstract

Corrosion Protection of Injection Molded Porous 440C Stainless Steel by Electroplated Zinc Coating

Zinc electroplating was used to enhance corrosion resistance of porous metal injection molded 440C stainless steel. Controlled porosity was achieved by the powder space holder technique and by using sodium chloride as a space holder material. The internal pore structure of porous 440C was deposited by zinc using electroplating with three different electrolytes of zinc acetate, zinc sulfate, and zinc chloride. Our results show that all zinc depositions on porous 440C samples significantly improved corrosion resistance. The lowest corrosion was observed with zinc acetate at 30 wt.% porosity. The developed zinc coated porous 440C samples have potential in applications in corrosive environments.

Surface Acoustic Wave Biosensor with Laser-Deposited Gold Layer Having Controlled Porosity

Laser-deposited gold immobilization layers having different porosities were incorporated into love wave surface acoustic wave sensors (LW-SAWs). Variation of pulsed laser deposition parameters allows good control of the gold film morphology. Biosensors with various gold film porosities were tested using the biotin–avidin reaction. Control of the Au layer morphology is important since the biotin and avidin layer morphologies closely follow that of the gold. The response of the sensors to biotin/avidin, which is a good indicator of biosensor performance, is improved when the gold layer has increased porosity. Given the sizes of the proteins, the laser-deposited porous gold interfaces have optimal pore dimensions to ensure protein stability.

Fabricating Lattice Structures via 3D Printing: The Case of Porous Bio-Engineered Scaffolds

Over time, the fabrication of lattice, porous structures has always been a controversial field for researchers and practitioners. Such structures could be fabricated in a stochastic way, thus, with limited control over the actual porosity percentage. The emerging technology of 3D printing, offered an automated process that did not require the presence of molds and operated on a layer-by-layer deposition basis, provided the ability to fabricate almost any shape through a variety of materials and methods under the umbrella of the ASTM terminology “additive manufacturing”. In the field of biomedical engineering, the technology was embraced and adopted for relevant applications, offering an elevated degree of design freedom. Applications range in the cases where custom-shaped, patient-specific items have to be produced. Scaffold structures were already a field under research when 3D printing was introduced. These structures had to act as biocompatible, bioresorbable and biodegradable substrates, where the human cells could attach and proliferate. In this way, tissue could be regenerated inside the human body. One of the most important criteria for such a structure to fulfil is the case-specific internal geometry design with a controlled porosity percentage. 3D printing technology offered the ability to tune the internal porosity percentage with great accuracy, along with the ability to fabricate any internal design pattern. In this article, lattice scaffold structures for tissue regeneration are overviewed, and their evolution upon the introduction of 3D printing technology and its employment in their fabrication is described.

Fabrication and evaluation of porous and conductive nanofibrous scaffolds for nerve tissue engineering

Peripheral nerve repair is still one of the major clinical challenges which has received a great deal of attention. Nerve tissue engineering is a novel treatment approach that provides a permissive environment for neural cells to overcome the constraints of repair. Conductivity and interconnected porosity are two required characteristics for a scaffold to be effective in nerve regeneration. In this study, we aimed to fabricate a conductive scaffold with controlled porosity using polycaprolactone (PCL) and chitosan (Chit), FDA approved materials for the use in implantable medical devices. A novel method of using tetrakis (hydroxymethyl) phosphonium chloride (THPC) and formaldehyde was applied for in situ synthesis of gold nanoparticles (AuNPs) on the scaffolds. In order to achieve desirable porosity, different percentage of polyethylene oxide (PEO) was used as sacrificial fiber. Fourier transform infrared spectroscopy (FTIR) and field emission scanning electron microscopy (FE-SEM) results demonstrated the complete removing of PEO from the scaffolds after washing and construction of interconnected porosities, respectively. Elemental and electrical analysis revealed the successful synthesis of AuNPs with uniform distribution and small average diameter on the PCL/Chit scaffold. Contact angle measurements showed the effect of porosity on hydrophilic properties of the scaffolds, where the porosity of 75–80% remarkably improved surface hydrophilicity. Finally, the effect of conductive nanofibrous scaffold on Schwann cells morphology and vaibility was investigated using FE-SEM and MTT assay, respectively. The results showed that these conductive scaffolds had no cytotoxic effect and support the spindle-shaped morphology of cells with elongated process which are typical of Schwann cell cultures.