Basic Principles and Recent Advances in Magnetic Cell Separation

Magnetic cell separation has become a key methodology for the isolation of target cell populations from biological suspensions, covering a wide spectrum of applications from diagnosis and therapy in biomedicine to environmental applications or fundamental research in biology. There now exists a great variety of commercially available separation instruments and reagents, which has permitted rapid dissemination of the technology. However, there is still an increasing demand for new tools and protocols which provide improved selectivity, yield and sensitivity of the separation process while reducing cost and providing a faster response. This review aims to introduce basic principles of magnetic cell separation for the neophyte, while giving an overview of recent research in the field, from the development of new cell labeling strategies to the design of integrated microfluidic cell sorters and of point-of-care platforms combining cell selection, capture, and downstream detection. Finally, we focus on clinical, industrial and environmental applications where magnetic cell separation strategies are amongst the most promising techniques to address the challenges of isolating rare cells.

On the Principles of Design of Transport Nodes

The methodology developed by the authors refers to designing, calculating, and optimising transport nodes based on the original systemic approach as a main method. The use of the methodology will make it possible to design transport nodes more rationally and to evaluate their development projects more correctly.A «system» is understood as a general natural form of structuring organised substance, which enables it to function stably in a changeable environment. The basic principles are formulated as follows: the system consists of elements, each of which is also a system; active self-maintenance is developed in the system, that is, active actions are counteracting external adverse influences; it is shown that self-maintenance is provided by adaptability, and in transport systems the self-maintenance is particularly provided by adaptive technology.A contradiction (a dialectical one) arises: on the one hand, the elements are independent systems that have their own system parameters and mechanisms for their active maintenance, and on the other hand, they are subordinate creatures capable of flexibly changing their work to maintain the parameters of the supersystem. It is necessary to find harmony between the levels of development of these opposite properties. Transport nodes are also considered from these systemic positions. Exposition of several definitions of nodes by leading national scientists is followed by a statement showing that they all contradict the new systemic approach.Suggested system definition of a node describes it as a set of stations. The authors also propose a new classification of transport nodes, formulate criteria for their rational design depending on the classes, and propose correct design and optimisation principles.

Differences in the transient responses of individual glaciers: a case study of the Cascade Mountains of Washington State, USA

Mountain glaciers have response times that govern retreat due to anthropogenic climate change. We use geometric attributes to estimate individual response times for 383 glaciers in the Cascade mountain range of Washington State, USA. Approximately 90% of estimated response times are between 10 and 60 years, with many large glaciers on the short end of this distribution. A simple model of glacier dynamics shows that this range of response times entails consequential differences in recent and ongoing glacier changes: glaciers with decadal response times have nearly kept pace with anthropogenic warming, but those with multi-decadal response times are far from equilibrium, and their additional committed retreat stands well beyond natural variability. These differences have implications for changes in glacier runoff. A simple calculation highlights that transient peaks in area-integrated melt, either at the onset of forcing or due to variations in forcing, depend on the glacier's response time and degree of disequilibrium. We conclude that differences in individual response times should be considered when assessing the state of a population of glaciers and modeling their future response. These differences in response can arise simply from a range of different glacier geometries, and the same basic principles can be expected in other regions as well.

Electronic Corpus of Linguaculture of the Northern Angara Region: Foundations, Structure, and Application

Linguaculture is a heterogeneous linguacognitive phenomenon that depends on the local area, values, and community, as well as on national and ethno-specifics cultural codes and subcodes of mythology, theology, anthropomorphism, biomorphism, household, commerce, etc. The article introduces the linguacultural corpus of the Northern Angara Region that unites oral and multimedia (polycode) texts of traditional regional linguaculture. The corpus is being developed at the Siberian Federal University and includes empirical material of complex folklore, dialectological, and ethnographic expeditions to Kezhemskiy, Boguchany and Motygino areas. The article describes the basic principles of the corpus, i.e. representativeness, synchronism, and regionality, as well as the thematic, genre, functional, and semantic diversity of oral and polycode texts. Culturally marked oral texts are included in the corpus based on their linguistic and cultural informativeness. The corpus is divided into dialect, folklore, and multimedia subcorpuses of valuable information, which can solve various issues of Russian studies, as well as practical issues of linguacultural ecology. The information search process depends on several parameters: speech and folklore genre, topic, basic concept, and word. The article also contains a comparative analysis with other specialized corpora of oral speech, which revealed prospects for further development and application. The linguacultural corpus of the Northern Angara Region contributes to regional lexicography and other branches of Russian studies, e.g. Siberian linguaculture, language and culture, language and mentality, etc.

Social justice, health equity, and mental health

There is considerable evidence to indicate that stigma and discrimination against people with mental illnesses are widely prevalent across nations. Research also shows that individuals with mental illnesses are likely to die 15–20 years younger than those who do not have these illnesses. In addition, they are more likely to experience delays in help-seeking leading to poor outcomes and are more likely to experience physical illnesses. Stigma and discrimination appear to play a major role in depriving people with mental illnesses of their basic rights. Their economic, political, social, and human rights are often ignored. In this article, we describe the capability to be healthy and basic principles of social justice related to mental health. We discuss findings of discrimination often embedded in laws of countries around the world in the context of basic human rights. We believe that clinicians have a key role as advocates for their patients. Clinicians and policymakers need to work together to bring about social and health equity.

Australian Uniform Evidence Law

Now in its second edition, Australian Uniform Evidence Law provides a clear, accessible introduction to the law of evidence. Following the structure of the Evidence Act 1995 (Cth), the text introduces students to basic principles, then covers more complex elements of evidence law. Cases and excerpts from legislation have been selected to guide students through the application of the Act. This edition has been updated to include significant recent case examples and decisions. Each chapter includes a summary of key points, definitions and practice questions to encourage students to apply their knowledge to realistic scenarios. The final chapter comprises longer-form, complex problems designed to test students' understanding of the concepts and rules covered in the Act as a whole. Guided solutions to each question are provided so students can check their understanding. Providing clear explanations and examples, Australian Uniform Evidence Law is an essential resource for all students of evidence law.

Spontaneous Facial Expressions and Micro-expressions Coding: From Brain to Face

Facial expressions are a vital way for humans to show their perceived emotions. It is convenient for detecting and recognizing expressions or micro-expressions by annotating a lot of data in deep learning. However, the study of video-based expressions or micro-expressions requires that coders have professional knowledge and be familiar with action unit (AU) coding, leading to considerable difficulties. This paper aims to alleviate this situation. We deconstruct facial muscle movements from the motor cortex and systematically sort out the relationship among facial muscles, AU, and emotion to make more people understand coding from the basic principles: We derived the relationship between AU and emotion based on a data-driven analysis of 5,000 images from the RAF-AU database, along with the experience of professional coders.We discussed the complex facial motor cortical network system that generates facial movement properties, detailing the facial nucleus and the motor system associated with facial expressions.The supporting physiological theory for AU labeling of emotions is obtained by adding facial muscle movements patterns.We present the detailed process of emotion labeling and the detection and recognition of AU.Based on the above research, the video's coding of spontaneous expressions and micro-expressions is concluded and prospected.

Perbandingan Manajemen Kurikulum Sebelum Dan Selama Pandemi Covid-19 Di Sekolah Dasar Islam Mohammad Hatta

The curriculum is the core subject of education. Oliva mentioned "curriculum that is thought" (what), in the form of programs, plans, materials to be taught (content). Meanwhile, the meaning of learning (instruction) is more of "means used to teach that which is thought" (how), in the form of methods, teaching strategies, teaching approaches, implementation of learning in class and outside the classroom. Once the importance of curriculum management as the main management substance in schools, the basic principles of curriculum management emphasize that the learning process can run well, by measuring the achievement of goals by students and encouraging teachers to develop and continuously perfect learning strategies carried out in class.The curriculum plays an important role in realizing quality and quality schools, to create a quality curriculum, empowerment in curriculum management is needed. Management of the institutional-level curriculum needs to be coordinated by the leadership which is developed integrally in the context of School-Based Management and the Education Unit Level Curriculum and is adjusted to the vision and mission of the Educational Institution. The SDI Mohammad Hatta curriculum is intended for all school members, especially students, educators and education personnel, the formation of school culture can be carried out by schools through a series of planning activities, implementation of learning that is more student-oriented, comprehensive assessment of school-level planning in essence is strengthening the preparation at SDI Mohammad Hatta level, such as establishing a vision, mission, goals, curriculum structure, academy calendar and syllabus preparation. This type of qualitative research uses descriptive research methods. The purpose of this study was to describe the curriculum management at SDI Mohammad Hatta and to describe how the education unit level curriculum was before and during the Covid-19 pandemic.

Rules and regulations review on micro-takaful scheme development in Malaysia

Purpose This study aims to review Although the features of the first micro-takaful are slightly different from the customary long-established takaful settings, the rules and regulations remain unchanged following the original guidelines of operating insurance and takaful. Until Perlindungan Tenang makes its first premiere, the rules and regulations on micro-takaful are gaining ground. The dissimilarity of micro-takaful from the original takaful calls for updated guidelines, so that any micro-takaful scheme launched in the market meets the demand and needs of the targeted population. the current rules and regulations on micro-takaful in Malaysia to determine whether it addresses the basic principles of micro-takaful. Design/methodology/approach This study uses content analysis as the best method to review each guideline in the related rules and regulations across several documents such as microinsurance and micro-takaful discussion paper and guidelines on family takaful products. Findings Overall, the findings reveal that guidelines on micro-takaful operating in Malaysia support the micro-takaful requirement to be affordable, valuable, accessible, understandable and simplified. Matching the rules and regulations with this population feedback, the extended distribution channel may need further scrutiny due to deficit trust among public members toward insurance and takaful. Originality/value The insights presented are of important illumination to achieve long-term sustainability financial protection while preserving human well-being among those underserved.