Flooding Causes Dramatic Compositional Shifts and Depletion of Putative Beneficial Bacteria on the Spring Wheat Microbiota

Flooding affects both above- and below-ground ecosystem processes, and it represents a substantial threat for crop and cereal productivity under climate change. Plant-associated microbiota play a crucial role in plant growth and fitness, but we still have a limited understanding of the response of the crop-microbiota complex under extreme weather events, such as flooding. Soil microbes are highly sensitive to abiotic disturbance, and shifts in microbial community composition, structure and functions are expected when soil conditions are altered due to flooding events (e.g., anoxia, pH alteration, changes in nutrient concentration). Here, we established a pot experiment to determine the effects of flooding stress on the spring wheat-microbiota complex. Since plant phenology could be an important factor in the response to hydrological stress, flooding was induced only once and at different plant growth stages (PGSs), such as tillering, booting and flowering. After each flooding event, we measured in the control and flooded pots several edaphic and plant properties and characterized the bacterial community associated to the rhizosphere and roots of wheat plant using a metabarcoding approach. In our study, flooding caused a significant reduction in plant development and we observed dramatic shifts in bacterial community composition at each PGS in which the hydrological stress was induced. However, a more pronounced disruption in community assembly was always shown in younger plants. Generally, flooding caused a (i) significant increase of bacterial taxa with anaerobic respiratory capabilities, such as members of Firmicutes and Desulfobacterota, (ii) a significant reduction in Actinobacteria and Proteobacteria, (iii) depletion of several putative plant-beneficial taxa, and (iv) increases of the abundance of potential detrimental bacteria. These significant differences in community composition between flooded and control samples were correlated with changes in soil conditions and plant properties caused by the hydrological stress, with pH and total N as the soil, and S, Na, Mn, and Ca concentrations as the root properties most influencing microbial assemblage in the wheat mircobiota under flooding stress. Collectively, our findings demonstrated the role of flooding on restructuring the spring wheat microbiota, and highlighted the detrimental effect of this hydrological stress on plant fitness and performance.

Responses of Soil Microbial Biomass and Enzyme Activities to Natural Restoration of Reclaimed Temperate Marshes After Abandonment

Restoration of reclaimed marshes has great effects on soil biological processes. However, the responses of soil microbial properties (microbial biomass and enzyme activities) to natural restoration of reclaimed marshes is poorly studied, especially in a long restoration chronosequence. This study assessed the responses of soil microbial properties to natural restoration and investigated the relationships between soil microbial properties and soil physico-chemical and plant properties. We selected a restoration chronosequence (1, 4, 8, 13, 17, 27 years) after farmland abandonment, a soybean field, and a natural marsh in Sanjiang Plain, northeast China. For each site, we analyzed the soil microbial biomass carbon and nitrogen (MBC and MBN), four enzymes (β-glucosidase, invertase, catalase, urease) activities, soil physico-chemical properties at 0–50 cm depths, and plant properties (biomass, height, and coverage). The MBC and MBN contents increased with restoration time, but MBN content slowed down after 8 years of restoration. After 27 years of restoration, the soil MBC and MBN contents were 15.7 and 3.2 times of those in the soybean field, but the largest contents of MBC and MBN in the restored sites were 7.78%, 27.76% lower than those in natural marshes, respectively. Moreover, soil enzyme activities and the geometric mean of enzymatic activities (GME) also increased with restoration but slowed down after 13 years of restoration. After 27 years of restoration, the GME was 2.9 times than that in the soybean field, but the largest GME in the restored sites was 31.15% lower than that in the natural marsh. MBC and MBN contents, soil enzyme activities, and GME had significant relationships with soil C:N ratio, organic carbon, nutrients (total nitrogen, available nitrogen, total phosphorus), bulk density, moisture content, pH, plant properties, (i.e. biomass, height, and coverage) (p < 0.01). Redundancy analysis revealed that soil C:N ratio, pH, moisture content, total nitrogen and phosphorus were main factors affecting MBC and MBN contents and enzyme activities. In conclusion, soil microbial properties can respond positively to the natural restoration process of the reclaimed marshes and were significantly correlated with specific parameters of soil physico-chemical and plant properties.

Earthworm Inoculation Improves Upland Rice Crop Yield and Other Agrosystem Services in Madagascar

The effects of earthworm inoculation and cropping systems on upland rice systems were examined over a four-year period in the Highlands of Madagascar. Each year, endogeic earthworms Pontoscolex corethrurus (Rhinodrilidae) were inoculated (EW+) at a density of 75 ind m−2 or were not inoculated (EW0). Inoculation was tested in three cropping systems: conservation agriculture (CA) and traditional tillage with or without residues restitution. Soil and plant properties were measured during the first three years while soil biological properties were assessed at the fourth year. At the end of the experiment, earthworm density was three-fold higher in EW+ than in EW0, demonstrating the success of the inoculation. Earthworm density was more important in CA than in tillage systems. Earthworm inoculation had higher significant effects on soil and plant properties than cropping systems. Earthworm inoculation had positive effects on soil macroaggregation (+43%), aboveground biomass (+27%), rice grain yield (+45%), and N grain amount (+43%). Intensifying earthworm activity in field conditions to meet the challenge of ecological transition is supported by our study.

Names of Trees in East Slavic Charms

This paper continues the series of the authors’ works on the toponymy of charms previously published in Voprosy Onomastiki. It deals with proper names of trees (dendronyms) in East Slavic spells coming from East Belarusian, East Ukrainian, West and South Russian territories where the considered folklore tradition is particularly prominent. The naming of revered trees is a fairly common cult practice that occurs through cultural semiotization of significant plant properties: the most important features are the size and spreading of a tree, its situational connection with a person, the place of growth, etc. The folklore tradition (as reflected in charms) of naming trees follows the same logic, however, direct evidence of dendronyms “migration” from the real toponymic system to the texts of charms is lacking. The origins of mythodendronyms are established mainly in folklore, book and manuscript tradition. The paper explores functional features of charms that include names of trees (typically these are healing charms against fever and a snakebite). Hence, a classification of dendronyms from the point of view of their origin is proposed: deanthroponymic names (Yevim oak, Erofei tree, Katyarina byarosa, etc.), toponym-based names (Mamvriy oak, Buyan oak, Lukomor oak, etc.), ethnonym-based names (Sorochinsky oak, Vereisk oak, etc.), names derived form appellatives (karcolist tree, starodub oak, Rakitaniy oak, Kindyashnoe tree, and many others). The paper proposes etymological and motivational solutions for most of the mythodendronyms known to the authors, many of which are “opaque” and have not yet received a reliable interpretation.

Application of Multi-Component Conditioner with Clinoptilolite and Ascophyllum nodosum Extract for Improving Soil Properties and Zea mays L. Growth and Yield

The application of various conditioners in agriculture is one of the management practices used to improve soil quality and plant growth and development. The aim of this study was to assess the effect of a multi-component conditioner on the selected soil properties and maize (Zea mays L.) growth and yield. The effect of a conditioner on a set of soil properties and maize growth and yield was studied in one-year experiments carried out at three study sites, which were under a conventional tillage system. All of the study sites were located on farms in three geographic mezoregions in the Kuyavian-Pomeranian Region (Midwestern Poland). The studied soils were Haplic Luvisol (Janocin and Kobylnica) and Albic Luvisols (Krukówko) that were composed of sandy loam. A one-way analysis of variance (ANOVA) was used to determine the effect of a conditioner Solactiv on the soil and plant properties. The conditioner significantly affected the soil enzyme activities such as dehydrogenase (DHA), fluorescein sodium salt hydrolysis level (FDAH) and carboxymethylcellulose cellulase (CMC—cellulase); wherein the last one increased by about 16–20%. The application of Solactiv also increased the available K content (about 11%) but not the content of the microbial biomass C and N. Total porosity (TP), which was significantly higher in the soil treated with conditioner than in the control soils, increased the available water capacity (AWC) (about 2.2%). The higher AWC in the treated soil indicated the greater contribution of the mesopores in the TP (about 4%). A significantly higher readily available water capacity (RAWC) and small pores available water capacity (SAWC) was determined in the treated soils compared to the controls. Of the plant properties, only plant height, fresh cob biomass (BBCH 87–89) and fresh plant biomass (BBCH 84–85) were significantly increased by the conditioner. The application of Solactiv is considered to be a promising approach for developing sustainable agriculture by improving the soil’s biological activity and water-related properties.