EXTRACTION OF ELASTIC, VISCOUS AND INERTIAL COMPONENTS FROM THE TOTAL REACTION OF AN ADDITIONAL SUPPORT ATTACHED TO A RECTANGULAR PLATE

The paper deals with a mechanical system consisting of a hinged rectangular plate and an additional viscoelastic support with considering its mass-inertia. The impact of the characteristics of additional support on the plate strained state is studied by an original approach of extracting elastic, viscous and inertial components from the total reaction. The plate is assumed to be medium thickness, elastic and isotropic. The Timoshenko hypothesis is used for deformation equations. The external non-stationary force initiates plate vibrations. The impact of the additional support is replaced by the action of three unknown independent non-stationary concentrated forces. The basic formulas for deriving system of three Volterra integral equations are proposed. The system is then solved by numerical and analytical method. By discretizing in time the system of Volterra integral equations is reduced to a system of matrix equations. The system of matrix equations is solved with using generalized Kramer’s algorithm for block matrices and Tikhonov’s regularization method. Note that the approach proposed is applicable for other objects with additional supports, such as beams, plates and shells having various boundary contour and boundary supporting. The results of computing elastic, viscous and inertial components of total reactions on the plate are given. The approach proposed is verified by matching the results of computations by two different methods, namely numerical and analytical for one total reaction and numerical for the total reaction obtained by adding elastic, viscous and inertial components.

Optimization of the qPCR temperature profile is essential for efficient and rapid detection of Sars-CoV-2

qPCR protocols should specify shorter annealing and extension times to optimize the efficacy of the target product. These specific times must be long enough for the complexes to form at the correct binding site, but if these times are too long, it can result in unspecified products. Our results show that reducing the reaction time during the second cycle can increase the specificity of the resulting product and thus reduce the total reaction time. The overall temperature profile, optimized for the target gene, shortens the detection time, allowing faster and more specific detection of the presence of the Sars-CoV-2.

New insights into the molecular mechanism behind mannitol and erythritol fructosylation by β-fructofuranosidase from Schwanniomyces occidentalis

The β-fructofuranosidase from Schwanniomyces occidentalis (Ffase) is a useful biotechnological tool for the fructosylation of different acceptors to produce fructooligosaccharides (FOS) and fructo-conjugates. In this work, the structural determinants of Ffase involved in the transfructosylating reaction of the alditols mannitol and erythritol have been studied in detail. Complexes with fructosyl-erythritol or sucrose were analyzed by crystallography and the effect of mutational changes in positions Gln-176, Gln-228, and Asn-254 studied to explore their role in modulating this biocatalytic process. Interestingly, N254T variant enhanced the wild-type protein production of fructosyl-erythritol and FOS by $$\sim$$ ∼ 30% and 48%, respectively. Moreover, it produced neokestose, which represented $$\sim$$ ∼ 27% of total FOS, and yielded 31.8 g l−1 blastose by using glucose as exclusive fructosyl-acceptor. Noteworthy, N254D and Q176E replacements turned the specificity of Ffase transferase activity towards the synthesis of the fructosylated polyols at the expense of FOS production, but without increasing the total reaction efficiency. The results presented here highlight the relevance of the pair Gln-228/Asn-254 for Ffase donor-sucrose binding and opens new windows of opportunity for optimizing the generation of fructosyl-derivatives by this enzyme enhancing its biotechnological applicability.

Identifikasi Kebutuhan Masyarakat Nusa Tenggara Barat pada Pandemi Covid-19 di Media Sosial dengan Metode Crawling

People can write to the government about what they want through social media in the freedom of information era. Especially in the Covid-19 pandemic, the public's desire for government attention is getting higher. The purpose of this activity is to collect community needs at the beginning of the pandemic. The period of information retrieval is from March 30 to April 5, 2020. The activity uses the crawling method, which is gathering information with browser tools. The implementation stage starts from taking information with selenium, identifying posts and comments with Html tags, calculating the emergence of issues with N-gram NLP, and analyzing sentiment with Naive Bayes and Support-Vector Machine. The results showed that the community did not have panic to need foods with a total reaction of 172. The community was more worried about the spread of outbreaks from outside NTB with a reaction of 1,421.

New insights into the molecular mechanism behind mannitol and erythritol fructosylation by β-fructofuranosidase from Schwanniomyces occidentalis

The β-fructofuranosidase from Schwanniomyces occidentalis (Ffase) is a useful biotechnological tool for the fructosylation of different acceptors to produce fructooligosaccharides (FOS) and fructo-conjugates. In this work, the structural determinants of Ffase involved in the transfructosylating reaction of the alditols mannitol and erythritol have been studied in detail. Complexes with fructosyl-erythritol or sucrose were analyzed by crystallography and the effect of mutational changes in positions Gln-176, Gln-228, and Asn-254 revised to explore their role in modulating this biocatalytic process. Interestingly, N254T variant enhanced the wild-type protein production of fructosyl-erythritol and FOS by `30% and 48%, respectively. Moreover, it showed a shift towards neokestose, which represented ~27% of total FOS, and yielded 31.8 g l-1 blastose by using glucose as exclusive fructosyl-acceptor. Noteworthy, N254D and Q176E replacements turned the specificity of Ffase transferase activity towards the synthesis of the fructosylated polyols at the expense of FOS production, but without increasing the total reaction efficiency. The results presented here highlight the relevance of the pair Gln-228/Asn-254 for Ffase donor-sucrose binding and opens new windows of opportunity for optimizing the generation of fructosyl-derivatives by this enzyme enhancing its biotechnological applicability.

A Comparative Usability Study of Bare Hand Three-Dimensional Object Selection Techniques in Virtual Environment

Object selection is the basis of natural user–computer interaction (NUI) in a virtual environment (VE). Among the three-dimensional object selection techniques employed in virtual reality (VR), bare hand-based finger clicking interaction and ray-casting are two convenient approaches with a high level of acceptance. This study involved 14 participants, constructed a virtual laboratory environment in VR, and compared the above two finger-based interaction techniques in terms of aspects of the task performance, including the success rate, total reaction time, operational deviation, and accuracy, at different spatial positions. The results indicated that the applicable distance range of finger clicking interaction and finger ray-casting was 0.2 to 1.4 m and over 0.4 m, respectively. Within the shared applicable distance, the finger clicking interaction achieved a shorter total reaction time and higher clicking accuracy. The performance of finger clicking interaction varied remarkably at the center and edge of the horizontal field of view, while no significant difference was found among ray-casting at various horizontal azimuths. The current findings could be directly applied to the application of bare-hand interaction in VR environments.