The growth and development depending on the pre-sowing sunflower treatment method in the southern chestnut soils zone of the Volgograd region

The work contains the results of research carried out in 2015…2017, the seeds pre-sowing treatment methods influence in the alternating voltage electric field with the intensity of 8 kV/cm with an exposure of 60 seconds, by a Zerebra Agro growth regulator with an aqueous solution of 100 ml/1 liter of water. The decrease in the period from sowing to germination by 1 day, the decrease in the duration of the germination phase - basket formation by 3…5 days in the NK Neoma hybrid, by 1 day in the LG 5550 hybrid, by 2 days in the EU Petunia hybrid was established. The duration of the basket formation phase - flowering in the NK Neoma hybrid increased significantly in the EU Petunia hybrid by 6…7 days, not significantly in the LG 5550 hybrid by 1 day. The flowering-maturation phase lasted 51…56 days for the NK Neoma hybrid, 49…52 days for the LG 5550 hybrid, and 42…46 days for the EU Petunia hybrid. The duration of the germination-maturation phase in the NK Neoma hybrid increased by 2…3 days, in the LG 5550 hybrid by 2…3 days.

Petrogenesis and mode of emplacement of a Neoarchean tonalite-trondhjemite-diorite (TTD) suite: the Eau Jaune Complex, Abitibi greenstone belt

Magmatism during the maturation phase of Archean greenstone belts produced voluminous tonalite-trondhjemite-granodiorite (TTG) suites, as well as a lesser amount of tonalite-trondhjemite-diorite (TTD) suites. Such TTD suites have recently been recognized in the Archean Abitibi greenstone belt, on the southern flank of the Superior Craton, Canada, but their source(s), differentiation processes and depths of emplacement remain poorly constrained. The Neoarchean Eau Jaune Complex (EJC) lies in the northeastern corner of the Abitibi greenstone belt and represents one of the most voluminous tonalite-dominated and diorite-bearing intrusive suites of the Chibougamau region. This TTD suite comprises six intrusive phases with distinct petrology and chemistry. All units were emplaced as laccolith-like intrusions injected along discontinuities within the volcanic succession at ca. 2724 Ma (U-Pb zircon dating), during the synvolcanic interval (i.e., construction and maturation phase), at a depth of approximately 7–8 km. The most HREE-depleted phases (granodiorite, tonalite and trondhjemite) correspond to magmas that fractionated amphibole and were likely produced by partial melting of a garnet- and titanate-bearing amphibolite, akin to TTG magmas. The least HREE-depleted phases are dioritic in composition and correspond to mantle-derived magmas that may have interacted with TTG melts. This indicates interaction between coeval mantle-derived and crustal melts during the maturation phase of the Abitibi greenstone belt. Models formulated to address the geodynamic evolution of greenstone belts must account for the coeval production of basalt-derived (TTG suites) and mantle-derived (tholeiitic magmatism) melts occasionally interacting to form TTD suites.

Clove Oil Has the Activity to Inhibit Middle, Maturation and Degradation Phase of Candida Tropicalis Biofilm Formation

Clove oil is one of the natural antibacterial ingredients that is easily obtained because of its abundant amounts in nature. Various researches have been conducted, but the antibiofilm activity against Candida tropicalis has never been done. This study evaluates the effectiveness of clove oil in inhibiting and degrading C. tropicalis JFM 1541 biofilm activity. The research was conducted using the microtiter broth method. The antibiofilm activity was determined as the minimum biofilm inhibitory concentration (MBIC50), the minimum value of biofilm eradication concentration (MBEC50). Antibiofilm mechanism was elucidated using scanning electron microscopy (SEM). Statistical analyzes were performed using ANOVA (p <0.05). Showed that clove oil could inhibit biofilm formation at the middle phase by 65% (65.21 ± 0.01) and at the maturation phase by 56% (56.11 ± 0.01). Clove oil with a concentration of 1% v/v has been shown to have activity in degrading 41% of C. tropicalis biofilms (41.87 ± 0.01). SEM shows that clove oil can cause damage in the extracellular polymeric matrix (EPS) of C. tropicalis biofilm. In conclusion, clove oil acts as a potential antibiofilm activity against C. tropicalis (compared to nystatin as control drugs) and further developed a new antibiofilm agent.

Degradation of Film and Rigid Bioplastics During the Thermophilic Phase and the Maturation Phase of Simulated Composting

The recent regulations, which impose limits on single use plastics and packaging, are encouraging the development of bioplastics market. Some bioplastics are labelled as compostable with the organic waste according to a specific certification (EN 13432), however the conditions of industrial composting plants are generally less favourable than the standard test conditions. Aiming at studying the effective degradation of marketable bioplastic products under composting, the current research stresses novel elements which can strongly influence bioplastics degradation: the simulation of industrial composting conditions and the thickness of bioplastic products, ranging between 50 and 500 µm. The research approaches these critical aspects simulating a composting test of 20 days of thermophilic phase followed by 40 days of maturation phase, on starch-based polymer Mater-Bi® (MB), polybutylene adipate terephthalate (PBAT), polylactic acid (PLA) of different thickness. Conventional low density polyethylene (LDPE) was introduced as negative control. An overall study with Fourier Transform InfraRed (FTIR), ThermoGravimetric Analysis (TGA), Gel Permeation Chromatography (GPC), Scanning Electron Microscope (SEM) and visual inspections was applied. Results highlighted that MB film presented the highest degradation rate, 45 ± 4.7% in terms of weight loss. Both MB and PBAT were subjected to physico-chemical features change, while LDPE presented slight degradation signs. The most critical observations have been done for PLA, which is strongly influenced both by thickness and thermophilic phase duration, shorter than the EN 13432 conditions.

Multi-omic rejuvenation of human cells by maturation phase transient reprogramming

Ageing is the gradual decline in organismal fitness that occurs over time leading to tissue dysfunction and disease. At the cellular level, ageing is associated with reduced function, altered gene expression and a perturbed epigenome. Somatic cell reprogramming, the process of converting somatic cells to induced pluripotent stem cells (iPSCs), can reverse these age-associated changes. However, during iPSC reprogramming somatic cell identity is lost, and can be difficult to reacquire as re-differentiated iPSCs often resemble foetal rather than mature adult cells. Recent work has demonstrated that the epigenome is already rejuvenated by the maturation phase of reprogramming, which suggests full iPSC reprogramming is not required to reverse ageing of somatic cells. Here we have developed the first “maturation phase transient reprogramming” (MPTR) method, where reprogramming factors are expressed until this rejuvenation point followed by withdrawal of their induction. Using dermal fibroblasts from middle age donors, we found that cells reacquire their fibroblast identity following MPTR, possibly as a result of persisting epigenetic memory at enhancers. Excitingly, our method substantially rejuvenated multiple cellular attributes including the transcriptome, which was rejuvenated by around 30 years as measured by a novel transcriptome clock. The epigenome, including H3K9me3 histone methylation levels and the DNA methylation ageing clock, was rejuvenated to a similar extent. The magnitude of rejuvenation instigated by MTPR is substantially greater than that achieved in previous transient reprogramming protocols. MPTR fibroblasts produced youthful levels of collagen proteins, suggesting functional rejuvenation. Overall, our work demonstrates that it is possible to separate rejuvenation from pluripotency reprogramming, which should facilitate the discovery of novel anti-ageing genes and therapies.HighlightsWe developed a novel method by which human fibroblasts are reprogrammed until the maturation phase of iPSCs and are then returned to fibroblast identityDNA methylation memory in fibroblast enhancers may allow recovery of cell identity when fibroblast gene expression programmes are already extinctMolecular measures of ageing including transcriptome and DNA methylation clocks and H3K9me3 levels reveal robust and substantial rejuvenationFunctional rejuvenation of fibroblasts by MPTR is suggested by reacquisition of youthful levels of collagen proteins

Histology Analysis of Tissue Regeneration Process of Digit Tip Mice (Mus musculus) post amputation

The ability to regenerate tissue is different for each organism. Mice (Mus musculus) able to regenerate the 3rd phalange of a digit. The tissue regeneration process has four phases are the wound-healing phase, the blastema phase, the regeneration phase, and the maturation phase. Each phase has a different process and different activity of cells. Histological analysis is very important to see the activity of each cell in each phase of tissue regeneration. Through histological analysis we can find out the role of each cell in the tissue regeneration process as well as the processes that occur in tissue regeneration. In this study, we analyzed tissue histology in the digit tip mice at each regeneration phase post amputated. The phalanges were amputated on the 3rd phalanges of digit tip of 24 male mice which had been previously sedated using ketamine / xylazine. Digit tip were allowed to grow and regenerate, and samples were taken on days 0, 1, 3, 5, 10, 15, 25 after amputation. Histological analysis was performed using Hematoxylin-eosin staining on a sample preparation that had been made into paraffin blocks first. The histological showed that at the beginning of the wound, the tissue rapidly forms a thin epidermal layer to cover the wound. In the wound healing phase, some of embryonal cells proliferated and migrated actively. In the blastema phase, granule cells cluster to form various new tissues. In the regeneration phase, new tissue begins to form, such as blood vessel, muscle, bone, and epidermal tissue. In the regeneration phase on day 15, several new tissues have begun to form, such as blood vessel tissue, muscle, hemorrhoid, bone and epidermis. Finally, in the maturation phase on day 25, the tissue morphology process occurs and perfecting the digit tip mice tissue.

Biostimulant alleviates abiotic stress of mango grown in semiarid environment

ABSTRACT The shoot maturation phase is important for growing mango tree because it precedes the floral induction, when plants are under stress caused by high temperatures and low water availability, that could be reduced by using plant biostimulant. Thus, the current study aimed to use a plant biostimulant containing yeast extract and amino acids to alleviate abiotic stress in mango plants, cv. Tommy Atkins, grown in semiarid environment. The experimental design consisted of randomized blocks with five treatments, five repetitions and five plants per plot. Treatments consisted of: T1) two foliar sprays with [biostimulant + KCl] + two foliar sprays with K2SO4; T2) No biostimulant and four foliar sprays with K2SO4; T3) three individual foliar sprays with biostimulant and one foliar spray with K2SO4; T4) two foliar sprays with biostimulant and two foliar sprays with K2SO4; and T5) two foliar sprays with [biostimulant + K2SO4] + one foliar spray with K2SO4. The variables evaluated were: i) leaf concentrations of soluble carbohydrates, starch, amino acids and proline; and ii) enzymatic activity of catalase and ascorbate peroxidase. The foliar spray with biostimulant during the branch maturation phase of mango trees grown in tropical semiarid environment alleviated plant abiotic stress. Three foliar biostimulant sprays and one K2SO4spray are recommended to alleviate mango plant abiotic stress in semiarid environment.

Ecophysiology with barley eceriferum (cer) mutants: the effects of humidity and wax crystal structure on yield and vegetative parameters

Background and Aims In addition to preventing water loss, plant cuticles must also regulate nutrient loss via leaching. The eceriferum mutants in Hordeum vulgare (barley) potentially influence these functions by altering epicuticular wax structure and composition. Methods Cultivar ‘Bonus’ and five of its cer mutants were grown under optimal conditions for vegetative growth and maturation, and nine traits were measured. Nutrient and water amounts going through the soil and the amount of simulated rain as deionized water, affecting phyllosphere humidity, delivered during either the vegetative or maturation phase, were varied. Cer leaf genes and three wilty (wlt) mutations were characterized for reaction to toluidine blue and the rate of non-stomatal water loss. Key Results Vegetative phase rain on ‘Bonus’ significantly decreased kernel weight and numbers by 15–30 %, while in cer.j59 and .c36 decreases of up to 42 % occurred. Maturation phase findings corroborated those from the vegetative phase. Significant pleiotropic effects were identified: cer.j59 decreased culm and spike length and 1000-kernel weight, .c36 decreased kernel number and weight, .i16 decreased spike length and .e8 increased culm height. Excepting Cer.zv and .ym mutations, none of the other 27 Cer leaf genes or wlt mutations played significant roles, if any, in preventing water loss. Cer.zv and .ym mutants lost non-stomatal water 13.5 times faster than those of Cer.j, .yi, .ys and .zp and 18.3 times faster than those of four cultivars and the mutants tested here. Conclusions Using yield to measure the net effect of phyllosphere humidity and wax crystal structure revealed that the former is far more important than the latter. The amenable experimental setup described here can be used to delve deeper. Significant pleiotropic effects were identified for mutations in four Cer genes, of which one is known to participate in wax biosynthesis. Twenty-seven Cer leaf genes and three wlt mutations have little if any effect on water loss.

39. Efficacy of Jatropha cream (Jatropha curcas L.) on maturation phase of cutaneus healing process in Mice (Mus Musculus)

This study aims to determine the efficacy of jatropha curcas L. cream in the maturation phase of healing of mice leather injuries. This study used nine rats with 3 treatments, ie treatment group I smeared cream base (P1), treatment group II smeared 0.1% sulfadiazine cream (P2) and treatment group III smeared jatropha curcas 10% (P3). The result of ANAVA test of treatment I, treatment II and treatment III had significant effect (P 0,05) on fibroblast cells but each treatment group on collagen density level showed significant effect (P 0.01). Duncan test results on fibroblasts, P3 was significantly different (P 0.05) with P2 and P3, whereas the results on P3 collagen density were significantly different (P 0.01) with P2 and P1. The conclusion that 10% jatropha gum cream for 10 days can decrease the number of fibroblast cells and increase the amount of collagen thus accelerate the process of maturation phase of healing of mice leather injuries.