Isolation of viable Babesia bovis merozoites to study parasite invasion

Babesia parasite invades exclusively red blood cell (RBC) in mammalian host and induces alterations to host cell for survival. Despite the importance of Babesia in livestock industry and emerging cases in humans, their basic biology is hampered by lack of suitable biological tools. In this study, we aimed to develop a synchronization method for Babesia bovis which causes the most pathogenic form of bovine babesiosis. Initially, we used compound 2 (C2), a specific inhibitor of cyclic GMP-dependent protein kinase (PKG), and a derivative of C2, ML10. While both inhibitors were able to prevent B. bovis egress from RBC and increased percentage of binary forms, removal of inhibitors from culture did not result in a synchronized egress of parasites. Because using PKG inhibitors alone was not efficient to induce a synchronized culture, we isolated viable and invasive B. bovis merozoites and showed dynamics of merozoite invasion and development in RBCs. Using isolated merozoites we showed that BbVEAP, VESA1-export associated protein, is essential for parasite development in the RBC while has no significant role in invasion. Given the importance of invasion for the establishment of infection, this study paves the way for finding novel antigens to be used in control strategies against bovine babesiosis.

Reciprocal predicates: a prototype model

Many languages have verbal stems like hug and marry whose intransitive realization is interpreted as reciprocal. Previous semantic analyses of such reciprocal intransitives rely on the assumption of symmetric participation. Thus, 'Sam and Julia hugged' is assumed to entail both 'Sam hugged Julia' and 'Julia hugged Sam'. In this paper we report experimental results that go against this assumption. It is shown that although symmetric participation is likely to be preferred by speakers, it is not a necessary condition for accepting sentences with reciprocal verbs. To analyze the reciprocal alternation, we propose that symmetric participation is a typical feature connecting the meanings of reciprocal and binary forms. This accounts for the optionality as well as to the preference of this feature. Further, our results show that agent intentionality often boosts the acceptability of sentences with reciprocal verbs. Accordingly, we propose that intentionality is another typical semantic feature of such verbs, separate from symmetric participation.

BINARY OR NON-BINARY? GENDER MORPHOLOGY IN SPANISH: DIFFERENCES DEPENDENT ON THE TASK

There is empirical evidence in different languages on how the computation of gender morphology during psycholinguistic processing affects the conformation of sex-generic representations. However, there is no empirical evidence on the processing of non-binary morphological variants in Spanish (-x or -e) in contrast to the generic masculine variant (-o). To analyze this phenomenon, we conducted two experiments: an acceptability judgment task and a sentence comprehension task. The results show differences depending on the task. So, the underlying processes that are put into play in each one generate different effects. In acceptability judgments, which involve strategic processes mediated by beliefs and the linguistic norm, the generic masculine is more acceptable to refer to mixed groups. In the sentence comprehension task, which inquires about automatic processes and implicit representations, the non-binary forms consistently elicited a reference to mixed groups. Furthermore, the response times indicated that these morphological variants do not entail a higher processing cost than the generic masculine.

Assessing the Broader Value of Planted Forests to Inform Forest Management Decisions

This study highlights the importance of incorporating objectively quantified, non-market environmental values (such as avoided erosion and carbon sequestration) into land use decision making for sustainable forest management. A continuously developing approach that has facilitated discussions between researchers, industries, and governments on the quantification of non-market values is the ecosystem services (ES) framework. Using a spatial economic tool, called Forest Investment Framework, this study is, to the best of our knowledge, the first assessment of the market (timber) and non-market (carbon sequestration, avoided nitrogen leaching and avoided erosion) ES values of the 1.75 million-hectare New Zealand planted forest estate. To collect the views of key planted forest industry representatives on ES assessment/quantification, we interviewed 14 forest managers representing 60% of the planted forest area. Results from the spatial economic analysis indicated that the non-market ES values can be more than four times the timber profit nationally, and up to 12 times higher in New Zealand’s most erosion-prone region. These estimated values are indicative and should be treated with caution. From a sensitivity analysis, we found that different discount rates significantly impact ES values, ratios, and distributions. Results from the interviews indicated that ES quantification helped inform decision making by supporting license to operate, while also signaling the development of a reward system for sustaining ES. Sixty-four percent of survey respondents identified the importance of quantifying ES in ecological terms and describing other non-market ES in spatial, qualitative, or binary forms. Overall, this study provided evidence of how estimated non-market ES values compare with market values and highlighted the importance of including them in decision making processes. Future cost benefit analyses that incorporate these non-market monetary ES values would complement multi-criteria analysis that integrate additional dimensions and allow decision makers to rank options based on their particular criteria.

Thue equations that simultaneously fail the Hasse principle

We refine a previous construction by Akhtari and Bhargava so that, for every positive integer m, we obtain a positive proportion of Thue equations F(x, y) = h that fail the integral Hasse principle simultaneously for every positive integer h less than m. The binary forms F have fixed degree ≥ 3 and are ordered by the absolute value of the maximum of the coefficients.

Semi-invariants of binary forms pertaining to a unimodality theorem of Reiner and Stanton

The symmetric difference of the [Formula: see text]-binomial coefficients [Formula: see text] was introduced by Reiner and Stanton. They proved that [Formula: see text] is symmetric and unimodal for [Formula: see text] and [Formula: see text] even by using the representation theory for Lie algebras. Based on Sylvester’s proof of the unimodality of the Gaussian coefficients, as conjectured by Cayley, we find an interpretation of the unimodality of [Formula: see text] in terms of semi-invariants. In the spirit of the strict unimodality of the Gaussian coefficients due to Pak and Panova, we prove the strict unimodality of the symmetric difference [Formula: see text], except for the two terms at both ends, where [Formula: see text], [Formula: see text] and at least one of [Formula: see text] and [Formula: see text] is even.