Evidence for widespread cytoplasmic structuring into mesoscopic condensates

Eukaryotic cytoplasm organizes itself via both membrane-bound organelles and membrane-less biomolecular condensates (BMCs). Known BMCs exhibit liquid-like properties and are typically visualized on the scale of ~1 um. They have been studied mostly by microscopy, examining select individual proteins. Here, we investigate the global organization of native cytoplasm with quantitative proteomics, using differential pressure filtration, size exclusion, and dilution experiments. These assays reveal that BMCs form throughout the cytosplasm, predominantly at the mesoscale of ~100 nm. Our data indicate that at least 18% of the proteome is organized via such mesoscale BMCs, suggesting that cells widely employ dynamic liquid-like clustering to organize their cytoplasm, at surprisingly small length scales.

Conditioning of Sewage Sludge with Physical, Chemical and Dual Methods to Improve Sewage Sludge Dewatering

The paper presents the impact of different methods of sewage sludge conditioning on the improvement of sludge dewatering during pressure filtration processes. The following conditioning methods were tested for sludge preparation: sonication, addition of organic and inorganic chemicals (Zetag 8180, PIX 113 and the combined action of both substances). The research covered: physical and chemical analysis of sewage sludge, measurement of capillary suction time as an indicator of sludge dewaterability, some technical parameters of sludge pressure filtration process and the analysis of filtrate to assess the degree of contamination. The results of the research showed that the final water content of the prepared sludge decreased, while the specific filtration resistance increased. Among the tested methods the best results of sludge dewatering effects were obtained for sonicated sludge and its preparation with inorganic coagulant PIX 113. The combined effect of sonication with the addition of chemicals Zetag 8180 and PIX113 to sludge allowed for the reduction of organic substances, ammonium nitrogen and phosphates in filtrate after sludge dewatering.