em tomography
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Author(s):  
C. Shan Xu ◽  
Song Pang ◽  
Gleb Shtengel ◽  
Andreas Müller ◽  
Alex T. Ritter ◽  
...  

SummaryUnderstanding cellular architecture is essential for understanding biology. Electron microscopy (EM) uniquely visualizes cellular structure with nanometer resolution. However, traditional methods, such as thin-section EM or EM tomography, have limitations inasmuch as they only visualize a single slice or a relatively small volume of the cell, respectively. Here, we overcome these limitations by imaging whole cells and tissues with enhanced Focus Ion Beam Scanning Electron Microscopy (FIB-SEM) in high resolution with month-long acquisition duration. We use this approach to generate reference 3D image datasets at 4-nm isotropic voxels for ten different examples, including cultured cells (cancer, macrophages, and T-cells) as well as tissues (mouse pancreatic islets and the Drosophila fan-shaped body). We open access to all datasets in OpenOrganelle, an interactive web platform that allows accessing both the original 3D EM data, and subsequent organelle segmentation. Together, these data will serve as a reference library to explore comprehensive quantification of whole cells and their constituents, thus addressing questions related to cell identities, cell morphologies, cell-cell interactions, as well as intracellular organelle organization and structure.


2020 ◽  
Vol 219 (9) ◽  
Author(s):  
Zhiming Chen ◽  
Sandra L. Schmid

Clathrin-mediated endocytosis occurs via the assembly of clathrin-coated pits (CCPs) that invaginate and pinch off to form clathrin-coated vesicles (CCVs). It is well known that adaptor protein 2 (AP2) complexes trigger clathrin assembly on the plasma membrane, and biochemical and structural studies have revealed the nature of these interactions. Numerous endocytic accessory proteins collaborate with clathrin and AP2 to drive CCV formation. However, many questions remain as to the molecular events involved in CCP initiation, stabilization, and curvature generation. Indeed, a plethora of recent evidence derived from cell perturbation, correlative light and EM tomography, live-cell imaging, modeling, and high-resolution structural analyses has revealed more complexity and promiscuity in the protein interactions driving CCP maturation than anticipated. After briefly reviewing the evidence supporting prevailing models, we integrate these new lines of evidence to develop a more dynamic and flexible model for how redundant, dynamic, and competing protein interactions can drive endocytic CCV formation and suggest new approaches to test emerging models.


2020 ◽  
Vol 21 (15) ◽  
pp. 5558
Author(s):  
Astrid Rollenhagen ◽  
Bernd Walkenfort ◽  
Rachida Yakoubi ◽  
Sarah A. Klauke ◽  
Sandra F. Schmuhl-Giesen ◽  
...  

Modern electron microscopy (EM) such as fine-scale transmission EM, focused ion beam scanning EM, and EM tomography have enormously improved our knowledge about the synaptic organization of the normal, developmental, and pathologically altered brain. In contrast to various animal species, comparably little is known about these structures in the human brain. Non-epileptic neocortical access tissue from epilepsy surgery was used to generate quantitative 3D models of synapses. Beside the overall geometry, the number, size, and shape of active zones and of the three functionally defined pools of synaptic vesicles representing morphological correlates for synaptic transmission and plasticity were quantified. EM tomography further allowed new insights in the morphological organization and size of the functionally defined readily releasable pool. Beside similarities, human synaptic boutons, although comparably small (approximately 5 µm), differed substantially in several structural parameters, such as the shape and size of active zones, which were on average 2 to 3-fold larger than in experimental animals. The total pool of synaptic vesicles exceeded that in experimental animals by approximately 2 to 3-fold, in particular the readily releasable and recycling pool by approximately 2 to 5-fold, although these pools seemed to be layer-specifically organized. Taken together, synaptic boutons in the human temporal lobe neocortex represent unique entities perfectly adapted to the “job” they have to fulfill in the circuitry in which they are embedded. Furthermore, the quantitative 3D models of synaptic boutons are useful to explain and even predict the functional properties of synaptic connections in the human neocortex.


2020 ◽  
Vol 117 (31) ◽  
pp. 18680-18691 ◽  
Author(s):  
Nuruddin Unchwaniwala ◽  
Hong Zhan ◽  
Janice Pennington ◽  
Mark Horswill ◽  
Johan A. den Boon ◽  
...  

For positive-strand RNA [(+)RNA] viruses, the major target for antiviral therapies is genomic RNA replication, which occurs at poorly understood membrane-bound viral RNA replication complexes. Recent cryoelectron microscopy (cryo-EM) of nodavirus RNA replication complexes revealed that the viral double-stranded RNA replication template is coiled inside a 30- to 90-nm invagination of the outer mitochondrial membrane, whose necked aperture to the cytoplasm is gated by a 12-fold symmetric, 35-nm diameter “crown” complex that contains multifunctional viral RNA replication protein A. Here we report optimizing cryo-EM tomography and image processing to improve crown resolution from 33 to 8.5 Å. This resolves the crown into 12 distinct vertical segments, each with 3 major subdomains: A membrane-connected basal lobe and an apical lobe that together comprise the ∼19-nm-diameter central turret, and a leg emerging from the basal lobe that connects to the membrane at ∼35-nm diameter. Despite widely varying replication vesicle diameters, the resulting two rings of membrane interaction sites constrain the vesicle neck to a highly uniform shape. Labeling protein A with a His-tag that binds 5-nm Ni-nanogold allowed cryo-EM tomography mapping of the C terminus of protein A to the apical lobe, which correlates well with the predicted structure of the C-proximal polymerase domain of protein A. These and other results indicate that the crown contains 12 copies of protein A arranged basally to apically in an N-to-C orientation. Moreover, the apical polymerase localization has significant mechanistic implications for template RNA recruitment and (−) and (+)RNA synthesis.


Author(s):  
Archan Ganguly ◽  
Florian Wernert ◽  
Sébastien Phan ◽  
Daniela Boassa ◽  
Utpal Das ◽  
...  

SUMMARYClathrin has established roles in endocytosis, with clathrin-cages enclosing membrane infoldings, followed by rapid disassembly and reuse of monomers. However, in neurons, clathrin synthesized in cell-bodies is conveyed into axons and synapses via slow axonal transport; as shown by classic pulse-chase radiolabeling. What is the cargo-structure, and mechanisms underlying transport and presynaptic-targeting of clathrin? What is the precise organization at synapses? Combining live-imaging, mass-spectrometry (MS), Apex-labeled EM-tomography and super-resolution, we found that unlike dendrites where clathrin transiently assembles/disassembles as expected, axons contain stable ‘transport-packets’ that move intermittently with an anterograde bias; with actin/myosin-VI as putative tethers. Transport-packets are unrelated to endocytosis, and the overall kinetics generate a slow biased flow of axonal clathrin. Synapses have integer-numbers of clathrin-packets circumferentially abutting the synaptic-vesicle cluster, advocating a model where delivery of clathrin-packets by slow axonal transport generates a radial organization of clathrin at synapses. Our experiments reveal novel trafficking mechanisms, and an unexpected nanoscale organization of synaptic clathrin.


2019 ◽  
Vol 375 (1792) ◽  
pp. 20190153 ◽  
Author(s):  
Susan K. Dutcher

The generation of ciliary waveforms requires the spatial and temporal regulation of dyneins. This review catalogues many of the asymmetric structures and proteins in the cilia of Chlamydomonas , a unicellular alga with two cilia that are used for motility in liquid medium. These asymmetries, which have been identified through mutant analysis, cryo-EM tomography and proteomics, provide a wealth of information to use for modelling how waveforms are generated and propagated. This article is part of the Theo Murphy meeting issue ‘Unity and diversity of cilia in locomotion and transport’.


SPE Journal ◽  
2019 ◽  
Vol 25 (01) ◽  
pp. 119-138 ◽  
Author(s):  
Yanhui Zhang ◽  
Femke C. Vossepoel ◽  
Ibrahim Hoteit

Summary An ensemble-based history-matching framework is proposed to enhance the characterization of petroleum reservoirs through the assimilation of crosswell electromagnetic (EM) data. As an advanced technology in reservoir surveillance, crosswell EM tomography can be used to estimate a cross-sectional conductivity map and associated saturation profile at an interwell scale by exploiting the sharp contrast in conductivity between hydrocarbons and saline water. Incorporating this information into reservoir simulation in combination with other available observations is expected to enhance the forecasting capability of reservoir models and to lead to better quantification of uncertainty. The proposed approach applies ensemble-based data-assimilation methods to build a robust and flexible framework in which various sources of available measurements can be integrated. A comparative study evaluates two different implementations of the assimilation of crosswell EM data. The first approach integrates the crosswell EM field components in their original form, which entails forward simulation of the observed EM responses from the simulated reservoir state. In the second approach, formation conductivity is derived from the EM data through inversion and is subsequently assimilated into the reservoir model. An image-oriented distance parameterization of the fluid front assimilates the conductivity field in an efficient and robust manner and overcomes issues with data size, errors, and their correlation. Numerical experiments for different test cases with increasing complexity provide insight into the performance of the two proposed integration schemes. The results demonstrate the efficiency of the developed history-matching workflow and the added value of crosswell EM data in enhancing the reservoir characterization and reliability of dynamic reservoir forecasts.


2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Marie-Christin Spindler ◽  
Josef Redolfi ◽  
Frederik Helmprobst ◽  
Philip Kollmannsberger ◽  
Christian Stigloher ◽  
...  

Abstract Telomere movements during meiotic prophase I facilitate synapsis and recombination of homologous chromosomes. Hereby, chromosome movements depend on the dynamic attachment of meiotic telomeres to the nuclear envelope and generation of forces that actively move the telomeres. In most eukaryotes, forces that move telomeres are generated in the cytoplasm by microtubule-associated motor proteins and transduced into the nucleus through the LINC complexes of the nuclear envelope. Meiotic LINC complexes, in mouse comprised of SUN1/2 and KASH5, selectively localize to the attachment sites of meiotic telomeres. For a better understanding of meiotic telomere dynamics, here we provide quantitative information of telomere attachment sites that we have generated with the aid of electron microscope tomography (EM tomography). Our data on the number, length, width, distribution and relation with microtubules of the reconstructed structures indicate that an average number of 76 LINC complexes would be required to move a telomere attachment site.


2019 ◽  
Author(s):  
Nida Farheen ◽  
Mukund Thattai

AbstractThe genome of the influenza virus consists of eight distinct single-stranded RNA segments, each encoding proteins essential for the viral life cycle. When the virus infects a host cell these segments must be replicated and packaged into new budding virions. The viral genome is assembled with remarkably high fidelity: experiments reveal that most virions contain precisely one copy of each of the eight RNA segments. Cell-biological studies suggest that genome assembly is mediated by specific reversible and irreversible interactions between the RNA segments and their associated proteins. However, the precise inter-segment interaction network remains unresolved. Here we computationally predict that tree-like irreversible interaction networks guarantee high-fidelity genome assembly, while cyclic interaction networks lead to futile or frustrated off-pathway products. We test our prediction against multiple experimental datasets. We find that tree-like networks capture the nearest-neighbor statistics of RNA segments in packaged virions, as observed by EM tomography. Just eight tree-like networks (of a possible 262,144) optimally capture both the nearest-neighbor data as well as independently measured RNA-RNA contact propensities. These eight do not include the previously-proposed hub-and-spoke and linear networks. Rather, each predicted network combines hub-like and linear features, consistent with evolutionary models of interaction gain and loss.


2019 ◽  
Author(s):  
Anthony D. Junker ◽  
Adam W. J. Soh ◽  
Eileen T. O’Toole ◽  
Janet B. Meehl ◽  
Mayukh Guha ◽  
...  

ABSTRACTMotile cilia generate directed hydrodynamic flow that is important for the motility of cells and extracellular fluids. To optimize directed hydrodynamic flow, motile cilia are organized and oriented into a polarized array. Basal bodies (BB) nucleate and position motile cilia at the cell cortex. Cytoplasmic BB-associated microtubules are conserved structures that extend from BBs. Using the ciliate, Tetrahymena thermophila, combined with EM-tomography and light microscopy, we show that BB-appendage microtubules assemble coincident with new BB assembly and are attached to the cell cortex. These BB-appendage microtubules are specifically marked with post translational modifications of tubulin, including glycylation. Mutations that prevent glycylation shorten BB-appendage microtubules and disrupt BB positioning and cortical attachment. Consistent with the attachment of BB-appendage microtubules to the cell cortex for BB positioning, mutations that disrupt the cellular cortical cytoskeleton similarly disrupt the cortical attachment and positioning of BBs. In summary, BB-appendage microtubules promote the organization of ciliary arrays through attachment to the cell cortex.SUMMARY STATEMENTBasal bodies position motile cilia at the cell cortex. This study finds tubulin glycylation to promote BB-associated microtubule elongation and structural attachment of basal bodies to the cell’s cortical cytoskeleton.


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