The Diversity of Bacteria Associated with the Invasive Gall Wasp Dryocosmus kuriphilus, Its Galls and a Specialist Parasitoid on Chestnuts

Dryocosmus kuriphilus (Hymenoptera: Cynipidae) induces galls on chestnut trees, which results in massive yield losses worldwide. Torymus sinensis (Hymenoptera: Torymidae) is a host-specific parasitoid that phenologically synchronizes with D. kuriphilus. Bacteria play important roles in the life cycle of galling insects. The aim of this research is to investigate the bacterial communities and predominant bacteria of D. kuriphilus, T. sinensis, D. kuriphilus galls and the galled twigs of Castanea mollissima. We sequenced the V5–V7 region of the bacterial 16S ribosomal RNA in D. kuriphilus, T. sinensis, D. kuriphilus galls and galled twigs using high-throughput sequencing for the first time. We provide the first evidence that D. kuriphilus shares most bacterial species with T. sinensis, D. kuriphilus galls and galled twigs. The predominant bacteria of D. kuriphilus are Serratia sp. and Pseudomonas sp. Furthermore, the bacterial community structures of D. kuriphilus and T. sinensis clearly differ from those of the other groups. Many species of the Serratia and Pseudomonas genera are plant pathogenic bacteria, and we suggest that D. kuriphilus may be a potential vector of plant pathogens. Furthermore, a total of 111 bacteria are common to D. kuriphilus adults, T. sinensis, D. kuriphilus galls and galled twigs, and we suggest that the bacteria may transmit horizontally among D. kuriphilus, T. sinensis, D. kuriphilus galls and galled twigs on the basis of their ecological associations.

Local-scale dispersal patterns and susceptibility to Dryocosmus kuriphilus in different Castanea species and hybrid clones: insights from a field trial

The chestnut gall wasp Dryocosmus kuriphilus is a major insect pest affecting chestnut trees worldwide. Medium and long-term control of this pest can be improved by using resistant or less susceptible cultivars and hybrid clones. In addition, little is known about the local patterns of dispersal of the pest. We obtained data from trees of 3 chestnut species and 27 hybrid clones in a field trial in NW Spain with the aim of evaluating the susceptibility of the material to the gall wasp and identifying possible drivers of local spatial dispersal. In the first 3 years of the invasion by D. kuriphilus, the number of trees attacked and the number of galls on each tree were spatially clustered. Tree height significantly predicted both variables, suggesting that gall wasps may use visual cues to locate suitable host trees, at least in the early stage of invasion. Assessment of the susceptibility of hybrid clones/pure species must take concurrent indicators of infestation levels into account. We suggest the use of indices involving galls on shoots because these enable good assessment of the damage to chestnut trees. The study findings add to existing knowledge on the susceptibility of hybrid chestnut clones. We report, for the first time, two hybrid clones resistant to the pest and one hybrid clone which exhibited consistently low values for all of the indicators of infestation level. The results have important implications regarding selection of plant material for use in afforestation in Spain, where the current high rate of chestnut planting is expected to continue.

Population Dynamics and Tree Damage of the Invasive Chestnut Gall Wasp, Dryocosmus kuriphilus, in Its Southernmost European Distributional Range

The invasive chestnut gall wasp (CGW), Dryocosmus kuriphilus, the worst pest of chestnut cultivation, has spread worryingly throughout Europe in less than 20 years. Despite the great concern around this pest, little is known about the status in its southernmost distribution in continental Europe. We assessed spatio-temporal patterns in the population dynamics, phenology and tree damage caused by CGW in southern Spain. Likewise, the relationship between these variables and thermal trends was evaluated. We found strong variation in the population dynamics and flight phenology among localities and over time, which were highly influenced by changes in thermal regimes. Specifically, warmer localities and vegetative periods promoted higher population densities, a partial increase in the survival of immature stages, and advanced flight activity. Moreover, tree damage evolved differently over time in each locality, which suggests that local conditions may determine differences in damage evolution. Our findings evidence that great spatio-temporal variability in the CGW populations takes place across invaded areas in its southernmost European distributional range. Although control mechanisms have been introduced, implementation of further control and management measures are critical to cope with this main threat for the chestnut industry and to prevent its spread to nearing chestnut-producing areas.

The Diversity and Dynamics of Fungi in Dryocosmus kuriphilus Community

Dryocosmus kuriphilus (Hymenoptera: Cynipidae) is a gall wasp that induces insect galls on chestnut trees and results in massive yield losses worldwide. Fungi can cause the necrosis of chestnut trees and the death of gall wasps. The aim of this research was to investigate the potential role of D. kuriphilus in the transmission of fungi. We sequenced the ribosomal RNA internal transcribed spacer region 1 of fungi in D. kuriphilus adults, associated insect galls and the galled twigs of Castanea mollissima, using high-throughput sequencing. We compared the species richness, α-diversity and community structure of fungi in D. kuriphilus adults, insect galls and the galled twigs. We provide the first evidence that D. kuriphilus adults shared most fungal species with associated insect galls and the galled twigs, and were dominated by Botryosphaeria sp., Aspergillus sp. and Diaporthe sp. We suggest D. kuriphilus adults may be potential vectors of plant pathogens and may facilitate the transmission of fungi between chestnut trees. Furthermore, the fungi may horizontally transmit among D. kuriphilus adults, associated insect galls and the galled twigs.

Fine Morphology of Antennal and Ovipositor Sensory Structures of the Gall Chestnut Wasp, Dryocosmus kuriphilus

Dryocosmus kuriphilus is a gall-inducing insect, which can cause significant damage on plants of the genus Castanea Mill., 1754. Antennae and ovipositor are the main sensory organs involved in the location of suitable oviposition sites. Antennal sensilla are involved in the host plant location, while ovipositor sensilla assess the suitability of the ovipositional bud. On both organs, diverse sensillar organs are present. Here, the distribution and ultrastructural organization of the sensilla were investigated by scanning and transmission electron microscopy. The antennae of D. kuriphilus are filiform and composed of 14 antennomeres, with the distal flagellomere bearing the highest number of sensilla. On the antennae, 6 sensilla types were found; sensilla chaetica, campaniformia, coeloconica-I, coeloconica-II, trichoidea and placoidea. The sensilla placoidea and trichoidea were the most abundant types. On the external walls of the ovipositor, gustatory and mechanoreceptive sensilla were observed. Internally, the egg channel hosted two additional sensory structures. The putative functional role of each sensilla in the context of insect’s ecology is discussed as well as the ovipositional mechanism used by this insect.