Diabrotica undecimpunctata virus 2, a Novel Small RNA Virus Discovered from Southern Corn Rootworm, Diabrotica undecimpunctata howardi Barber (Coleoptera: Chrysomelidae)

ABSTRACT The genome of Diabrotica undecimpunctata virus 2 (DuV2), a putative positive-sense, single-stranded RNA virus identified from the southern corn rootworm transcriptome, comprises 5,313 nucleotides, including a short poly(A) tail. The two open reading frames encode a nonstructural polyprotein (p156) and a putative capsid protein (p25).

Genome Sequence of a Small RNA Virus of the Southern Corn Rootworm, Diabrotica undecimpunctata howardi Barber (Coleoptera: Chrysomelidae)

ABSTRACT The genome sequence of a novel small RNA virus, tentatively named Diabrotica undecimpunctata virus 1 (DuV1), was discovered from the transcriptome of the southern corn rootworm, Diabrotica undecimpunctata howardi Barber. DuV1 has a positive-sense, single-stranded RNA genome that encodes a single polyprotein of 3,401 amino acids with limited similarity to other viruses.

Synthesis of corn rootworm pheromones from commercial diols

A mixture of stereoisomers of the corn rootworm pheromones was synthesised via the Grignard coupling of protected bromohydrins with alkylcuprate as a key step. The synthesis of 8-methyldec-2- yl propanoate (I ), the northern corn rootworm Diabrotica longicornis Say pheromone, was achieved from pentane-1,5-diol in four steps with an overall yield of 35.1 % and 10-methyltridecan-2-one (II ), the southern corn rootworm Diabrotica undecimpunctata howardi Barber pheromone, was synthesised from octane-1,8-diol as commercially available starting material in five steps with an overall yield of 28.7 %.

Spotted cucumber beetle Diabrotica undecimpunctata howardi Barber (Insecta: Coleoptera: Chrysomelidae)

Spotted cucumber beetle is a major agricultural pest of North America. Another name for the spotted cucumber beetle is “southern corn rootworm”. Many Diabrotica species cause damage to field crops, especially corn, making these beetles a major agricultural concern. Because of the subterranean nature of their larvae, these insects are hard and expensive to control. This 6-page fact sheet was written by Harsimran Kaur Gill, Gaurav Goyal, and Jennifer Gillett-Kaufman, and published by the UF Department of Entomology and Nematology, September 2013. http://edis.ifas.ufl.edu/in1008