DNA-based quantification of Fusarium oxysporum f. sp. vasinfectum in environmental soils to describe spatial variation in inoculum density

Fusarium wilt of cotton, caused by the soilborne fungal pathogen Fusarium oxysporum f. sp. vasinfectum (FOV), occurs in regions of the United States where cotton (Gossypium spp.) is grown. Race 4 of this pathogen (FOV4) is especially aggressive and does not require the co-occurrence of the root knot nematode (Meloidogyne incognita) to infect cotton. Its sudden appearance in far-west Texas in 2016 after many years of being restricted to California is of great concern, as is the threat of its continued spread through the cotton-producing regions of the United States. The aim of this research was to analyze the spatial variability of FOV4 inoculum density in the location where FOV4 is locally emerging, using quantitative and droplet digital polymerase chain reaction (qPCR and ddPCR) methods. Soil samples collected from a field with known FOV4 incidence in Fabens, Texas were analyzed. Appreciable variation in inoculum density was found to occur at spatial scales smaller than the size of plots involved in cultivar trial research, and was spatially autocorrelated (Moran’s I, Z = 17.73, p < 0.0001). These findings indicate that for cultivar trials, accounting for the spatial distribution of inoculum either by directly quantifying it or through the use of densely-distributed “calibration checks” is important to the interpretation of results.

Glandulicactus uncinatus var. wrightii (Cactaceae) with 15 cm spines

Central spines of Glandulicactus uncinatus var. wrightii have never been reported longer than 13 cm. I report plants from Anthony’s Nose in far West Texas with 15–16.5 cm central spines.

Mississippian southern Laurentia tuffs came from a northern Gondwana arc

A Paleozoic arc that formed by southward subduction of the Rheic oceanic plate beneath northern Gondwana has long been inferred, but its history and geochemical signatures remain poorly understood. New U-Pb ages, juvenile εHf signatures, and trace-element composition data of young zircons from tuffs at two southern Laurentia sites indicate their derivation from a continental arc that was active from ca. 328 to ca. 317 Ma and permit correlation of sedimentary sequences 800 km apart in southern Laurentia. These include the Stanley tuffs in the Ouachita Mountains of southeastern Oklahoma and southwestern Arkansas and the newly discovered Barnett tuff in the subsurface of the Midland Basin in west Texas (USA). The Barnett tuff has a zircon chemical abrasion–isotope dilution–thermal ionization mass spectrometry U-Pb date of 327.8 ± 0.8 Ma, similar to the oldest Stanley tuff in the Ouachita Mountains. Zircon Hf isotope depleted mantle model ages further suggest that the source was a continental arc on basement with both Grenville and Pan-African affinities, pointing to northern Gondwana or peri-Gondwana terranes. The new data link the tuffs to granitoids (326 Ma) of the Maya block in southern Mexico, which was part of northern Gondwana. Correlation of the Stanley-Barnett tuffs across southern Laurentia suggests the likely presence of Mississippian tuffs over a broad region in southern Laurentia, and their usefulness for constraining absolute ages of basin fills and characterizing the Gondwanan arc.

274 Comparison of Two Ultrasound Systems for Estimation of Carcass Attributes in Feedlot Steers

Sixty-eight (68) crossbred steers were evaluated using two real-time, B-mode ultrasound units to estimate final carcass attributes. The cattle were ultrasounded at the West Texas A&M research feedlot (Canyon, Texas) and carcass data was collected at the West Texas A&M Meats Laboratory (Canyon, Texas) or Tyson Fresh Meats (Amarillo, Texas). Objectives of the study were 1) to compare ultrasound live animal data to carcass attributes at harvest 2) to evaluate the performance differences between the ALOKA 500 (ALK) and the EVO ultrasound units All ultrasound images were captured by the same Ultrasound Guidelines Council (UGC) certified technician with images being processed at the UltraInsights Laboratory (Pierce, Colorado). The correlations between 12th rib fat thickness of the carcass (FTC) and ultrasound (FTU) were 0.84 for the ALK and 0.85 for the EVO, with no differences being found between the two units (P = 0.15). Correlations between the 12-13th rib carcass ribeye area (REAC) and ultrasound ribeye area (REAU) were 0.69 for the ALK and 0.66 for the EVO. There was no difference in REAU size between the two units. Carcass marbling score (MS) and ultrasound intramuscular fat (IMFU) correlations were 0.78 for the ALK and 0.84 for the EVO. The IMF data were found to be different between the two units, with the EVO measuring a mean IMFU value of 6.03 and the ALK a value of 5.26 with the change of 0.77 being different (P &lt; 0.01). It is concluded that both ultrasound units performed favorably when predicting FTC and marbling score but were not highly correlated for REAU. It was found that the predictions between the two units on FT and REA were the same, though the EVO was higher on both. The IMFU values between the ALK and EVO were different, with the EVO again predicting higher values.

The Aerosol Research Observation Station (AEROS)

Information on atmospheric particles’ concentration and sizes are important for environmental and human health reasons. Air quality monitor stations (AQMSs) for measuring Particulate Matter (PM) concentrations are found across the United States, but only three AQMSs measure PM2.5 concentrations (particles with an aerodynamic diameter of < 2.5 μm) in the Southern High Plains of West Texas (area ≥ 1.8 × 105 km2). This area is prone to many dust events (~21 per year), yet no information is available on other PM sizes, total particle concentration, or size distribution during these events. The Aerosol Research Observation Station (AEROS) was designed to continuously measure these particles’ concentrations to better understand the impact of dust events on local air quality. The AEROS aerosol measurements unit features a temperature-controlled shed with a dedicated inlet and custom-built dryer for each of the three aerosol instruments used. This article provides a description of AEROS as well as an intercomparison of the different instruments using laboratory and atmospheric particles, which shows that the instruments used provided similar concentration measurements. Measurement with AEROS can distinguish between various pollution events (natural dust events vs anthropogenic haze) to improve knowledge of the air quality in this region.

Middle Permian ostracods (Crustacea) from the Guadalupe Mountains, West Texas, USA

Middle Permian (Guadalupian) ostracods are described from the Williams Ranch Member of the Cutoff Formation (Roadian) and the McKittrick Canyon Member of the Bell Canyon Formation (Capitanian) from two sections exposed in Culberson County, Guadalupe Mountains, West Texas. Their taxonomy is discussed and adds to the scientific understanding of marine ostracod biodiversity and palaeobiogeography during the Middle Permian. Ostracod assemblages are represented by 51 species of 26 genera and 15 families. Eleven species are newly described: Healdia mckittrickensis Crasquin sp. nov., Healdia cutoffella Crasquin sp. nov., Aurikirkbya guadalupensis Crasquin sp. nov., Hollinella (Hollinella) williamsranchensis Crasquin sp. nov., Geisina culbersonensis Crasquin sp. nov., Paraparchites pecosensis Crasquin sp. nov., Bairdia elcapitanensis Forel sp. nov., Ceratobairdia mescaleroella Forel sp. nov., Ceratobairdia sexagintaduella Forel sp. nov., Ceratobairdia pratti Forel sp. nov., and Denticupachydomella bellcanyonensis Forel sp. nov. The diagnosis of the genus Denticupachydomella is emended. The palaeobiogeographic distribution of the species is analyzed and exemplifies the taxon exchanges between Tethyan and Panthalassic localities in the Permian. The eastern margin of the Palaeo-Tethyan realm is shown to have played a major role in radiation of taxa in the Permian.