Effect of Sirolimus on Intra-Abdominal adhesion development in a rat model

Objective: Postoperative intraabdominal adhesions still cause significant morbidity in surgical patients. This study aims to evaluate the effects of an immunosuppressor known as Sirolimus and an antiadhesive membrane which is formed with sodium hyaluronate carboxymethylcellulose-based bioresorbable membrane (Seprafilm™) to the intraabdominal adhesion formation in a rat model. Materials and Methods: This experimental study was performed at an experimental research center, Yeditepe University Faculty of Medicine, Istanbul. Spraque-Dawley Rats, at a weight of about 250±20 gr, were used. Group 1 (n=8): Abdomen was closed after applying cecal abrasion (control group), group 2 (n=8): 10 x 30 mm Seprafilm™ was applied under the abdominal wall after cecal abrasion ( Seprafilm™ group ). Group 3 (n =8): Sirolimus (0,5 mg/kg) was applied (Sirolimus group). Adhesions quantitatively evaluated by a blinded assessor according to the classification of Nair and his colleagues. Results: Statistically significant difference in terms of adhesion severity scores according to the Nair classification was found between the Sirolimus and the control group (p=0,03). Whereas, no statistically significant difference was found between the Seprafilm™ and the control group (p=0,17). Similarly, no statistically significant difference was found between Seprafilm™ and sirolimus group (p=0,64). Conclusion: Although there was no statistically significant difference between intraperitoneal application of Sirolimus and Seprafilm™ group (p = 0.57), a statistically significant difference was found when each group compared with the control group (p=0,03). Combined anti-adhesive effect of Sirolimus and Seprafilm™ can be evaluated in future studies.

The Impact of Double Layer Membrane Technique on Bone Regeneration in Postextraction Alveoli – A Pathohistological Experimental Study in Dogs

Post extraction alveolar ridge preservation is a preventive oral surgical procedure aimed to preserve the dimensions of the alveolar ridge after removal of the teeth. According to literature data, the utilization of guided bone regeneration for this purpose is absolutely justified. The aim of this study was to examine the effect of resorbable collagen membranes placed in two layers in respect to the application of membranes placed in one layer to the degree of bone regeneration after a tooth extraction. This experimental study was conducted on six adult dogs. In the first phase all four premolars were extracted on both sides in the lower jaw. After the volume-standardisation with bone drill, three post extraction alveoli were filled with bone substitute of equine origin and then covered with: collagen bioresor bable membrane of porcine origin; collagen bioresorbable membrane of equine origin; pericardial bioresorbable membrane of equine origin. The membranes on the left side were placed in single-layer and on the right side in double-layer manner. After different monitoring periods, histopathological analysis of the samples taken from the experimental regions was performed. A different degree of bone regeneration was achieved between experimental regions in which membranes were placed in one layer and those where membranes were placed in two layers. There was no difference between regions where membranes of different origin were placed in the same manner. The procedure of covering the post extraction alveoli, filled with bone substitute and with resorbable membranes placed in two layers resulted in a higher degree of bone regeneration compared to those where resorbable membranes were placed in one layer.

Clinical Evaluation of the Efficacy of Bioresorbable Membrane (Polyglactin 910) in the Treatment of Millers’ Class II Gingival Recession

Objective: The aim of the study was to determine the clinical outcome when polyglactin 910 membrane was used to treat 30 patients with isolated buccal millers class II gingival recessions. Materials and Method: Thirty subjects were selected with a chief complaint of hypersensitivity or denuded roots; all were willing to participate in the clinical study. Inclusion criteria were the presence of 4 mm or more of buccal millers class II gingival recession, a lack of contraindications for periodontal surgery. Results: The mean reduction in clinical recession from baseline to 6 months postoperatively was 2.47 ± 0.86 mm, i.e, 54%, which was statistically significant. There was a definite reduction in Pocket Depth, from a mean at baseline of 1.53 ± 0.63mm to a mean of 1.07 ± 0.26mm at 6 months. Conclusion: The use of GTR is a suitable alternative to the use of patient's own palatal masticatory mucosa. Polyglactin 910 as a GTR resorbable membrane gives the clinician another tool that provides acceptable root coverage.