rrna methylation
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2022 ◽  
pp. 101590
Author(s):  
Caraline Sepich-Poore ◽  
Zhong Zheng ◽  
Emily Schmitt ◽  
Kailong Wen ◽  
Zijie Scott Zhang ◽  
...  

2021 ◽  
Author(s):  
Zane T. Laughlin ◽  
Debayan Dey ◽  
Natalia Zelinskaya ◽  
Marta A. Witek ◽  
Pooja Srinivas ◽  
...  

Changes in bacterial ribosomal RNA (rRNA) methylation status can alter the activity of diverse groups of ribosome-targeting antibiotics. Typically, such modifications are incorporated by a single methyltransferase that acts on one nucleotide target and rRNA methylation directly prevents drug binding, thereby conferring drug resistance. However, loss of intrinsic methylation can also result in antibiotic resistance. For example, Mycobacterium tuberculosis (Mtb) becomes sensitized to tuberactinomycin antibiotics, such as capreomycin and viomycin, due to the action of the intrinsic methyltransferase TlyA. TlyA is unique among antibiotic resistance-associated methyltransferases as it has dual 16S and 23S rRNA substrate specificity and can incorporate cytidine-2'-O-methylations within two structurally distinct contexts. How TlyA accomplishes this feat of dual-target molecular recognition is currently unknown. Here, we report the structure of the Mtb 50S-TlyA subunit complex trapped in a post-catalytic state with a S-adenosyl-L-methionine analog using single-particle cryogenic electron microscopy. This structure, together with complementary site-directed mutagenesis and methyltransferase functional analyses, reveals critical roles in 23S rRNA substrate recognition for conserved residues across an interaction surface that spans both TlyA domains. These interactions position the TlyA active site over the target nucleotide C2144 which is flipped from 23S Helix 69 in a process stabilized by stacking of TlyA residue Phe157 on the adjacent A2143. This work reveals critical aspects of substrate recognition by TlyA and suggests that base flipping is likely a common strategy among rRNA methyltransferase enzymes even in cases where the target site is accessible without such structural reorganization.


2021 ◽  
Author(s):  
Noa Liberman ◽  
Maxim V. Gerashchenko ◽  
Konstantinos Boulias ◽  
Fiona G MacWhinnie ◽  
Albert Kejun Ying ◽  
...  

SummaryHeritable non-genetic information can regulate a variety of complex phenotypes. However, what specific non-genetic cues are transmitted from parents to their descendants are poorly understood. Here, we perform metabolic methyl-labelling experiments to track the heritable transmission of methylation from ancestors to their descendants in the nematode Caenorhabditis elegans. We find that methylation is transmitted to descendants in proteins, RNA, DNA and lipids. We further find that in response to parental starvation, fed naïve progeny display reduced fertility, increased heat stress resistance, and extended longevity. This intergenerational hormesis is accompanied by a heritable increase in N6’-dimethyl adenosine (m6,2A) on the 18S ribosomal RNA at adenosines 1735 and 1736. We identified the conserved DIMT-1 as the m6,2A methyltransferase in C. elegans and find that dimt-1 is required for the intergenerational hormesis phenotypes. This study provides the first labeling and tracking of heritable non-genetic material across generations and demonstrates the importance of rRNA methylation for regulating the heritable response to starvation.


2021 ◽  
Author(s):  
Zhuo Bi ◽  
Hong-Wei Su ◽  
Jia-Yao Hong ◽  
Babak Javid

SummaryDespite redundant cellular pathways to minimize translational errors, errors in protein synthesis are common. Pathways and mechanisms to minimize errors are classified as pre-ribosomal or ribosomal. Pre-ribosomal pathways are primarily concerned with appropriate pairing of tRNAs with their cognate amino acid, whereas to date, ribosomal proof-reading has been thought to only be concerned with minimizing decoding errors, since it has been assumed that the ribosomal decoding centre is blind to mischarged tRNAs. Here, we identified that in mycobacteria, deletion of the 16S ribosomal RNA methyltransferase gidB led to increased discrimination of mischarged tRNAs. GidB deletion was necessary but not sufficient for reducing mistranslation due to misacylation. Discrimination only occurred in mycobacteria enriched from environments or genetic backgrounds with high rates of mistranslation. Our data suggest that mycobacterial ribosomes are capable of discriminating mischarged tRNAs and that 16S rRNA methylation by GidB may act as a capacitor for moderating translational error.


2020 ◽  
Vol 85 (11) ◽  
pp. 1335-1349 ◽  
Author(s):  
I. A. Osterman ◽  
O. A. Dontsova ◽  
P. V. Sergiev

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Andrea Graziadei ◽  
Frank Gabel ◽  
John Kirkpatrick ◽  
Teresa Carlomagno

2’-O-rRNA methylation, which is essential in eukaryotes and archaea, is catalysed by the Box C/D RNP complex in an RNA-guided manner. Despite the conservation of the methylation sites, the abundance of site-specific modifications shows variability across species and tissues, suggesting that rRNA methylation may provide a means of controlling gene expression. As all Box C/D RNPs are thought to adopt a similar structure, it remains unclear how the methylation efficiency is regulated. Here, we provide the first structural evidence that, in the context of the Box C/D RNP, the affinity of the catalytic module fibrillarin for the substrate–guide helix is dependent on the RNA sequence outside the methylation site, thus providing a mechanism by which both the substrate and guide RNA sequences determine the degree of methylation. To reach this result, we develop an iterative structure-calculation protocol that exploits the power of integrative structural biology to characterize conformational ensembles.


2020 ◽  
Vol 48 (6) ◽  
pp. 3195-3210 ◽  
Author(s):  
Meijuan Zou ◽  
Ying Mu ◽  
Xin Chai ◽  
Min Ouyang ◽  
Long-Jiang Yu ◽  
...  

Abstract Methylation of nucleotides in ribosomal RNAs (rRNAs) is a ubiquitous feature that occurs in all living organisms. The formation of methylated nucleotides is performed by a variety of RNA-methyltransferases. Chloroplasts of plant cells result from an endosymbiotic event and possess their own genome and ribosomes. However, enzymes responsible for rRNA methylation and the function of modified nucleotides in chloroplasts remain to be determined. Here, we identified an rRNA methyltransferase, CMAL (Chloroplast MraW-Like), in the Arabidopsis chloroplast and investigated its function. CMAL is the Arabidopsis ortholog of bacterial MraW/ RsmH proteins and accounts to the N4-methylation of C1352 in chloroplast 16S rRNA, indicating that CMAL orthologs and this methyl-modification nucleotide is conserved between bacteria and the endosymbiont-derived eukaryotic organelle. The knockout of CMAL in Arabidopsis impairs the chloroplast ribosome accumulation and accordingly reduced the efficiency of mRNA translation. Interestingly, the loss of CMAL leads not only to defects in chloroplast function, but also to abnormal leaf and root development and overall plant morphology. Further investigation showed that CMAL is involved in the plant development probably by modulating auxin derived signaling pathways. This study uncovered the important role of 16S rRNA methylation mediated by CMAL in chloroplast ribosome biogenesis and plant development.


2020 ◽  
Vol 29 (1) ◽  
pp. 107-113
Author(s):  
Marek Ussowicz ◽  
Virginie Marcel ◽  
Flora Nguyen Van Long ◽  
Bernarda Kazanowska ◽  
Jean-Jacques Diaz ◽  
...  

2019 ◽  
Vol 25 (9) ◽  
pp. 1261-1265 ◽  
Author(s):  
Fatemeh Yeganeh Sefidan ◽  
Yalda Mohammadzadeh-Asl ◽  
Reza Ghotaslou

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