Developmental origins of cell heterogeneity in the human lung

The lung contains numerous specialized cell-types with distinct roles in tissue function and integrity. To clarify the origins and mechanisms generating cell heterogeneity, we created a first comprehensive topographic atlas of early human lung development. We report 83 cell states, several spatially-resolved developmental trajectories and predict cell interactions within defined tissue niches. We integrated scRNA-Seq and spatial transcriptomics into a web-based, open platform for interactive exploration. To illustrate the utility of our approach we show distinct states of secretory and neuroendocrine cells, largely overlapping with the programs activated either during lung fibrosis or small cell lung cancer progression. We define the origin of uncharacterized airway fibroblasts associated with airway smooth muscle in bronchovascular bundles, and describe a trajectory of Schwann cell progenitors to intrinsic parasympathetic neurons controlling bronchoconstriction. Our atlas provides a rich resource for further research and a reference for defining deviations from homeostatic and repair mechanisms leading to pulmonary diseases.

A Data-driven Platform for Simulating Vehicular Fog Computing Environment

<div>Edge/fog computing is a key enabling technology in 5G and beyond for fulfilling the tight latency requirements of compute-intensive vehicular applications such as cooperative driving. Concerning the spatio-temporal variation in the vehicular traffic flows and the demand for edge computing capacity generated by connected vehicles, vehicular fog computing (VFC) has been proposed as a cost-efficient deployment model that complements stationary fog nodes with mobile ones carried by moving vehicles. Accessing the feasibility and the applicability of such hybrid topology, and further planning and managing the networking and computing resources at the edge, require deep understanding of the spatio-temporal variations in the demand and the supply of edge computing capacity as well as the trade-offs between achievable Quality-of-Services and potential deployment and operating costs. To meet such requirements, we propose in this paper an open platform for simulating the VFC environment and for evaluating the performance and cost efficiency of capacity planning and resource allocation strategies under diverse physical conditions and business strategies. Compared with the existing edge/fog computing simulators, our platform supports the mobility of fog nodes and provides a realistic modeling of vehicular networking with the 5G and beyond network in the urban environment. We demonstrate the functionality of the platform using city-scale VFC capacity planning as example. The simulation results provide insights on the feasibility of different deployment strategies from both technical and financial perspectives.</div>

An open Platform for High-resolution Light-based Control of Microscopic Collectives

Engineering microscopic collectives of cells or microrobots is challenging due to the often-limited capabilities of the individual agents, our inability to reliably program their motion and local interactions, and difficulties visualising their behaviours. Here, we present a low-cost, modular and open-source Dynamic Optical MicroEnvironment (DOME) and demonstrate its ability to augment microagent capabilities and control collective behaviours using light. The DOME offers an accessible means to study complex multicellular phenomena and implement de-novo microswarms with desired functionalities. Corresponding author(s) Email: [email protected] [email protected]

Urban Open Platform for Borderless Smart Cities

As a network of connected sensors to transform data into knowledge, Urban Platforms have been rooted in several smart city projects. However, this has often resulted in them being no more than IoT dashboards. More recently, there has been an increased interest in supporting the data governance and distributed architecture of Urban Platforms in order to adjust these with the administrative structure in a specific city. In addition, Urban Platforms also deal with data roaming between different stakeholders including other cities, different government levels, companies and citizens. Nevertheless, the first deployments have led to an inflexible “smart cities in a box” approach that does not help with building digital skills and causes vendor lock-in to products that do not scale. There is a need to start with simple and widespread urban services through a collaborative joint cross-border, hands-on effort. In order to meet the level of interoperability, international standards should be adopted. The aim of an Urban Open Platform (UOP), introduced in this paper, is to support not only data acquisition but also various types of data processing: data is aggregated, processed, manipulated and extended within the city context. Conceptually, special attention has been put on scalability, roaming and reliability in urban environments. This article introduces the UOP uniquely in the cross-border data exchange context of two European capital cities, Helsinki and Tallinn, and validates it with 10 real-life urban use cases.

A Data-driven Platform for Simulating Vehicular Fog Computing Environment

<div>Edge/fog computing is a key enabling technology in 5G and beyond for fulfilling the tight latency requirements of compute-intensive vehicular applications such as cooperative driving. Concerning the spatio-temporal variation in the vehicular traffic flows and the demand for edge computing capacity generated by connected vehicles, vehicular fog computing (VFC) has been proposed as a cost-efficient deployment model that complements stationary fog nodes with mobile ones carried by moving vehicles. Accessing the feasibility and the applicability of such hybrid topology, and further planning and managing the networking and computing resources at the edge, require deep understanding of the spatio-temporal variations in the demand and the supply of edge computing capacity as well as the trade-offs between achievable Quality-of-Services and potential deployment and operating costs. To meet such requirements, we propose in this paper an open platform for simulating the VFC environment and for evaluating the performance and cost efficiency of capacity planning and resource allocation strategies under diverse physical conditions and business strategies. Compared with the existing edge/fog computing simulators, our platform supports the mobility of fog nodes and provides a realistic modeling of vehicular networking with the 5G and beyond network in the urban environment. We demonstrate the functionality of the platform using city-scale VFC capacity planning as example. The simulation results provide insights on the feasibility of different deployment strategies from both technical and financial perspectives.</div>

Amygdala Underlies the Environment Dependency of Defense Responses Induced via Superior Colliculus

In our previous study, we showed that the defense responses induced by the selective optogenetic activation of the uncrossed output pathway from the deeper layer of the superior colliculus were environment dependent in the mouse. In a small closed box, the stimulus frequently induced flight (fast forward run away) responses, while in a large open field, the stimulus tended to induce backward retreat responses. We tested a hypothesis that the amygdala is involved in such environment dependency of the innate defense responses. For this purpose, we made a bilateral lesion of the amygdala induced by the ibotenic acid injections in male mice. As a result, in the mice with lesions of substantial portions of the basolateral and basomedial complex, the flight responses in the closed box disappeared and retreat responses were mainly induced. The retreat responses on the open platform were unchanged. Classically, the amygdala has been considered to be involved in the memory-dependent contextual modulation of the fear responses. In contrast, the present results suggest a novel view on the role of the amygdala in which the amygdala plays a key role in sensing the current environmental setting for making a quick decision of action upon emergency, which is critical for survival in the natural environment.

Proposal for an IIoT Device Solution According to Industry 4.0 Concept

Today, Industrial Internet of Things (IIoT) devices are very often used to collect manufacturing process data. The integration of industrial data is increasingly being promoted by the Open Platform Communications United Architecture (OPC UA). However, available IIoT devices are limited by the features they provide; therefore, we decided to design an IIoT device taking advantage of the benefits arising from OPC UA. The design procedure was based on the creation of sequences of steps resulting in a workflow that was transformed into a finite state machine (FSM) model. The FSM model was transformed into an OPC UA object, which was implemented in the proposed IIoT. The OPC UA object makes it possible to monitor events and provide important information based on a client’s criteria. The result was the design and implementation of an IIoT device that provides improved monitoring and data acquisition, enabling improved control of the manufacturing process.

OPC UA TSN: a next-generation network for Industry 4.0 and IIoT

Purpose The purpose of this paper is to describe how emerging open standards are replacing traditional industrial networks. Current industrial Ethernet networks are not interoperable; thus, limiting the potential capabilities for the Industrial Internet of Things (IIoT). There is no forthcoming new generation fieldbus standard to integrate into the IIoT and Industry 4.0 revolution. The open platform communications unified architecture (OPC UA) time-sensitive networking (TSN) is a potential vendor-independent successor technology for the factory network. The OPC UA is a data exchange standard for industrial communication, and TSN is an Institute of Electrical and Electronics Engineers standard for Ethernet that supports real-time behaviour. The merging of these open standard solutions can facilitate cross-vendor interoperability for Industry 4.0 and IIoT products. Design/methodology/approach A brief review of the history of the fieldbus standards is presented, which highlights the shortcomings for current industrial systems in meeting converged traffic solutions. An experimental system for the OPC UA TSN is described to demonstrate an approach to developing a three-layer factory network system with an emphasis on the field layer. Findings From the multitude of existing industrial network schemes, there is a convergence pathway in solutions based on TSN Ethernet and OPC UA. At the field level, basic timing measurements in this paper show that the OPC UA TSN can meet the basic critical timing requirements for a fieldbus network. Originality/value This paper uniquely focuses on the specific fieldbus standards elements of industrial networks evolution and traces the developments from the early history to the current developing integration in IIoT context.

FOLIO and Smart Libraries: Meeting the future needs of Chinese Libraries

The vision and concept of FOLIO have gained the attention and expectation from libraries. Its open and flexible service platform provides an approach to the realization of the Smart Libraries in the future. On the basis of summarising the needs of Smart Libraries in China, the paper expounds the architecture and ideas of providing smart services based on FOLIO. By analyzing the problems and challenges in the implementation and localization of FOLIO in China, the author points out that the“Chinese Alliance for Library Service Platform”(also called YunHan Alliance) should play a very important role in running the Chinese FOLIO community. It should steward the direction of the community, carry out the minimum open source software application suite, cultivate an open platform ecology, so as to support the new business models for the prosperity of Chinese libraries in the future.