Qualitative and Quantitative Analysis of Tumor Cell Invasion Using Au Clusters

Tumor invasion/metastasis is still the major cause of death in cancer patients. Membrane type-1 matrix metalloproteinase (MT1-MMP) is directly related to tumor invasion/metastasis. To accurately and quickly distinguish the risk of invasion/metastasis of primary tumor cells, it is urgent to develop a simple and precise quantitative method to distinguish the expression level of MT1-MMP. In this work, we have constructed red fluorescent Au clusters with peroxidase-like properties that could specifically bind to MT1-MMP on human cervical cancer cells. After MT1-MMP was labelled with Au clusters, we could visually see red fluorescence of MT1-MMP on cervical cancer cells via fluorescence microscopy and catalytic color imaging using an ordinary optical microscope. The constructed Au clusters contained 26 Au atoms; thus, the amount of MT1-MMP on cervical cancer cells could be accurately quantified using inductively coupled plasma mass spectrometry (ICP-MS). More importantly, the invasion/metastasis capabilities of the cervical cancer Siha, Caski and Hela cells with different MT1-MMP amounts could be accurately distinguished by fluorescence/catalysis qualitative imaging and ICP-MS quantitative analysis. This method of qualitative/quantitative analysis of tumor-associated proteins on cancer cells has great potential for accurately diagnosing aggressive tumor cells and assessment of their invasion/metastasis risk.

Hybrid Metal-Dielectric-Metal Sandwiches for SERS Applications

The development of efficient plasmonic nanostructures with controlled and reproducible surface-enhanced Raman spectroscopy (SERS) signals is an important task for the evolution of ultrasensitive sensor-related methods. One of the methods to improving the characteristics of nanostructures is the development of hybrid structures that include several types of materials. Here, we experimentally investigate ultrathin gold films (3–9 nm) near the percolation threshold on Si/Au/SiO2 and Si/Au/SiO2/graphene multilayer structures. The occurring field enhanced (FE) effects were characterized by a recording of SERS signal from Crystal Violet dye. In this geometry, the overall FE principally benefits from the combination of two mechanisms. The first one is associated with plasmon excitation in Au clusters located closest to each other. The second is due to the gap plasmons’ excitation in a thin dielectric layer between the mirror and corrugated gold layers. Experimentally obtained SERS signals from sandwiched structures fabricated with Au film of 100 nm as a reflector, dielectric SiO2 spacer of 50 nm and ultrathin gold atop could reach SERS enhancements of up to around seven times relative to gold films near the percolation threshold deposited on a standard glass substrate. The close contiguity of the analyte to graphene and nanostructured Au efficiently quenches the fluorescent background of the model compound. The obtained result shows that the strategy of combining ultrathin nano-island gold films near the percolation threshold with gap plasmon resonances is promising for the design of highly efficient SERS substrates for potential applications in ultrasensitive Raman detection.

Associative and Dissociative Adsorption of N2O Molecule Over C24 Fullerene Doped With V, Mn, Ru, Pd and Rh: A DFT Investigation

DFT calculations using the PBE-D3 level of theory were conducted on the C24 and MC23 (M = V, Mn, Ru, Pd, Rh and Au) clusters in order to determine their stability and electronic and magnetic properties. The interaction of the N2O molecule with the above clusters has also been examined in order to assess their adsorption and catalytic properties. The results show that the reactivity of the C24 fullerene was greatly improved after doping with a TM atom, and the metal atom which was replaced the carbon atom in the pure C24 fullerene is considered as the most favorable site to adsorb and dissociate the N2O molecule. The molecular adsorption of the N2O on the surface of the MC23 clusters from its oxygen atom was found to be less energetically favorable than by nitrogen atom, and the calculated adsorption energies (Eads) are in the range of - 6.5 to - 24.7 kcal mol-1. The N2O adsorption on the metal site of the clusters could also lead to the dissociation of the N2O into O* and N2 molecule with adsorption energies which vary from - 2.3 to - 86.8 kcal mol-1. The mechanism of the N2O dissociation on the clusters surface was studied, and the activation energies for each system were calculated in order to find the most catalytically active clusters in the N2O decomposition. The results indicate that the activation energies of the VC23 and MnC23 clusters are much lower than those obtained for the other clusters, reflecting high catalytic activity of the two clusters in the N2O decomposition reaction compared to the other studied clusters. The values of Eac of the reaction over the VC23 and MnC23 cluster are 9.3 and 7.9 kcal mol-1, respectively, indicating that both clusters could be employed as greatly active and efficient nanocatalysts for the N2O decomposition reaction.

Hybrid Nanoparticles Based on Cobalt Ferrite and Gold: Preparation and Characterization

During the past few decades, hybrid nanoparticles (HNPs) based on a magnetic material and gold have attracted interest for applications in catalysis, diagnostics and nanomedicine. In this paper, magnetic CoFe2O4/Au HNPs with an average particle size of 20 nm, decorated with 2 nm gold clusters, were prepared using methionine as a reducer and an anchor between CoFe2O4 and gold. The methionine was used to grow the Au clusters to a solid gold shell (up to 10 gold deposition cycles). The obtained nanoparticles (NPs) were studied by X-Ray diffraction (XRD), transmission electron microscopy (TEM), Fourier-transform infrared (FT-IR) spectroscopy, X-Ray photoelectron spectroscopy (XPS) and UV-vis spectroscopy techniques. The TEM images of the obtained HNPs showed that the surface of cobalt ferrite was covered with gold nanoclusters, the size of which slightly increased with an increase in the number of gold deposition cycles (from 2.12 ± 0.15 nm after 1 cycle to 2.46 ± 0.13 nm after 10 cycles). The density of the Au clusters on the cobalt ferrite surface insignificantly decreased during repeated stages of gold deposition: 21.4 ± 2.7 Au NPs/CoFe2O4 NP after 1 cycle, 19.0 ± 1.2 after 6 cycles and 18.0 ± 1.4 after 10 cycles. The magnetic measurements showed that the obtained HNPs possessed typical ferrimagnetic behavior, which corresponds to that of CoFe2O4 nanoparticles. The toxicity evaluation of the synthesized HNPs on Chlorella vulgaris indicated that they can be applied to biomedical applications such as magnetic hyperthermia, photothermal therapy, drug delivery, bioimaging and biosensing.