Volatile fatty acids concentration and efficiency of microbial protein synthesis of concentrate cassava peel diet with different levels of protein sources

This study was conducted to evaluate the effects of different levels of protein sources in a concentrate cassava peel diet on volatile fatty acid (VFA) concentration and efficiency of microbial protein synthesis (EMPS) by in vitro method. The five diet treatments in this study consisted of cassava meal (CM), cassava peel (CP), cassava leaves (CL) and moringa leaves (ML) with the proportion applied were T1=CM70% + CP20% + CL5% + ML5%; T2=CM60% + CP20% + CL10% + ML10%; T3=CM50% + CP20% + CL15% + ML15%; T4=CM40% + CP20% + CL20% + ML20%; T5=CM30% + CP20% + CL25% + ML25%. The experiment used a randomized block design with five treatments and three replications. The results showed that increasing the level of protein sources in the ration tended to increase the value of total VFA, acetate, propionate, CO2, and CH4 in which T3 had the highest values. However, the effect of treatments were significant in EMPS value (P<0.01). It was concluded that increasing levels of cassava and moringa leaves in the concentrate cassava peel diet tended to increase the value of total VFA, acetate, propionate, butyrate, CO2, and CH4 which led to a significant increase in EMPS values.

Influence of Temperature and Screw Pressing on the Quality of Cassava Leaf Fractions

In this study, the development of a mild processing method for cassava leaves to remove cyanogenic compounds with minimum nutritional loss is evaluated. Fresh leaves were reduced in size using a mixer at temperatures of 25 (room temperature), 55, 80, and 100 °C for 1 min before screw pressing to separate the juice and press cake fractions. Cyanide content in the fresh leaves was reduced by 60% at 100 °C and by 57% in the juice sample processed at 25 °C. The press cake cyanide content was low (210 ppm) in both the control and the sample that was processed at 55 °C. An increase in the temperature for processing cassava leaves to 100 °C resulted in a loss of 5–13% of the CP and 7–18% of the vitamin C content. The press-cake fraction had high beta-carotene, lutein, and chlorophyll a and b content, and low values were registered for the juice fraction. Processing fresh cassava leaves at 25 and 55 °C resulted in fractions with high beta-carotene and lutein content. The protein quality of press cake was better than that of juice for feed. Short thermal shredding with pressing resulted in minimal loss of nutrients and a significant reduction of cyanide in the leaves.

Effects of Protein and Roughage Sources in Total Mixed Ration on Voluntary Feed Intake, Nutrient Intake and Blood Metabolites in Black Bengal Goats

Background: Agricultural productivity in Southeast Asian countries are important to produce food for human, thus the first priority to improve agricultural productivity is feed and feeding in livestock, especially in ruminants when rice straw and Napier grass are used as the roughage sources; in addition, cassava leaves and Leucaena leaves can be used as the protein sources. Interestingly, the strategy to improve feed in ruminant is through the use of total mixed rations (TMR) which are produced by combination of roughages, concentrates, minerals, vitamins and additives. Methods: During the period 2020-2021 a study with 2 × 2 factorial, in 4 × 4 Latin square design with 21 days per period tested the following TMR’s T1- 5% urea treated rice straw with dried Leucaena leaves, T2- 5% urea treated rice with dried cassava leaves, T3- fermented napier grass with dried Leucaena leaves and the T4- fermented napier grass with dried cassava leaves on voluntary feed intake, nutrient intake and blood metabolites in Black Bengal goats. Result: All treatments did not affect voluntary feed intake (kgDM/head/day and % BW) (P greater than 0.05), but feeding with 5% urea treated rice with dried cassava leaves, it was non significantly higher (0.71 kgDM/day). Furthermore, nutrient intake of organic matter (OM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and rumination were not affected among all the four treatments (P greater than 0.05). Additionally, blood glucose, blood urea nitrogen and triglyceride concentrations in plasma were not influenced due to treatments (P greater than 0.05). Nevertheless, Feed cost (USD/kg) was reduced T3 and T4. In conclusion, feeding Black Bengal goat with fermented napier grass with dried Leucaena leaves and fermented napier grass with dried cassava leaves in the TMR were suitable because of the lowest price (0.13 USD/kg) when compared to the other treatments.

The potential of cassava leaves extract in gingival fibroblasts of periodontitis of rats with ovarian dysfunction

Introduction: The prevalence of periodontitis has reached 42.8% in Indonesia. One contributing factor is Porphyromonas gingivalis. The ovarian dysfunction caused by ovariectomy procedures may also cause periodontitis. Post ovariectomy conditions resemble menopause, lowering the production of estrogen and progesterone hormones, leaving patients vulnerable to periodontitis. To prevent the side effects of the treatment, herbal ingredients are commonly used. The extract of cassava leaves is known to have pharmacological effects as an antioxidant and, thus, can be applied as a medicine for periodontitis. Methods: A total of 21 rats were divided into three groups, (1) control: healthy rats; (2) Group P.1: P. gingivalis-induced rats; (3) Group P.2: ovariectomized rats. Groups P.1 and P.2 were further divided into three subgroups that were administered cassava leaves extract at a dosage of 179.2 mg/ kg BW, aquades and vitamin C. Rats were euthanized at day eight after the initial treatment to collect left mandibular first molar. The mandibular sections were immunohistochemically stained to quantify SOD expression using light microscope while the Image J software was used. Fibroblast cells expressing SOD are characterized by brown coloration in the cytoplasm and darker nucleus. Results: In periodontitis conditions, either due to P.gingivalis induction or ovarian dysfunction, fibroblast cells in rats given cassava leaves extract expressed higher SOD than rats given aquades, but still lower than rats given vitamin C. The result of the one way ANOVA test showed p value of 0,00, which means that there is a significant difference in all groups. Conclusion: Cassava leaves extract demonstrated the potential to increase the antioxidant expression in P. gingivalis-induced and ovarian dysfunction rats.

Training for the Making of Singkong Leaves and Lele Fish for Communities in the Region of New Benda Kelurahan Pamulang-Tangerang Selatan

To increase community empowerment in the Benda Baru sub-district, Pamulang sub-district, South Tangerang City, in the form of product diversification of cassava leaves and catfish so that community welfare is created through optimizing the potential of cassava, processing various processed products of mashed beef jerky from cassava leaves and catfish that are varied, of high quality, nutritious and has a high selling value. This Community Service Program is aimed at the community as many as 16 mothers. The goal is to enable the community to plan and manage the potential of cassava to form/develop a group of people who are economically independent and prosperous. Outputs: (1) Products of cassava leaf jerky and catfish jerky (2) Publication of the activity of making pulverized cassava leaves and catfish jerky on YouTube. The method used in this activity is the provision of material, direct practice, and assistance. The presentation of the material was carried out in the local hall, with material on diversification of local food ingredients to introduce various alternative food sources and to introduce several examples of diversification of food based on existing local ingredients. The technique of making cassava leaves and catfish jerky is mentoring as well as the practice of making ground beef jerky and catfish jerky.

In vitro effect of aqueous extract of fresh cassava leaves (Manihot esculenta, Crantz) on the larvae of Haemonchus contortus as an intestinal parasite of sheep.

Cassava is frequently fed to animals. In the case of sheep, the producer relates consumption to a reduction in the parasite load. The literature has proven the effect of phenolic compounds as an anthelmintic in vivo, but no evidence for cyanogenic compounds, also present in all parts of the cassava plant, was found. A controlled in vitro bioassay was used to evaluate the aqueous extract of fresh cassava leaves. The efficiency parameter was the immobility of Haemonchus contortus larvae at the L3 stage, also used to evaluate commercial anthelmintics. Cell culture plates with 100 active L3-stage larvae per well were used, being each replicate constituted by three wells. Aqueous extract of fresh cassava leaves (FCL), Ivomec® 0.01% (PCI) as the positive control, and distilled water as negative control (NCW), were placed in the culture plate wells. Considering the immobility of the larvae as a positive anthelmintic effect, the results showed that in NCW treatments all larvae were mobile, while in PCI all 300 larvae were immobile. FCL produced a gradient of larval inactivation correlation (R2 0.996). The best-fit equation was y = -33.39ln(x) + 40.517, a logarithmic equation, which allowed the calculation of the Lethal Concentration (CL) of 3.44 µg CN- ml, or 80.0 mg of fresh cassava leaves per milliliter of water, with a performance equivalent to ivermectin. This concentration of free cyanide is compatible with the safe consumption of fresh leaves by live weight of sheep. The exact amount of cassava roots, leaves, or shoots, consumed to provide an effective dose for controlling H. contort should be established in vivo. Although phenolic compounds must also be present in the extract, the immobility was attributed to cyanogenic compounds since the correlation was proportional to the increase in the cyanide concentration. It can be concluded that the consumption of fresh cassava leaves has the potential as an anthelmintic agent to be evaluated in vivo by feeding sheep and goats. Local use could also add value to the production of fresh cassava leaves, with an average potential production of 2.5 tonnes ha-1, available throughout the year, with greater production at the beginning of cultivation and in the summer months. These leaves could be used after the roots harvesting or even after pruning for this purpose. Currently, this amount of good quality protein material remains without use in the field.

Effect of in vitro aqueous extract of fresh cassava leaves (Manihot esculenta, Crantz) on the larvae of Haemonchus contortus as intestinal parasite of sheep

Animals feeding with cassava is frequent. In the case of sheep, the producer relates consumption to a reduction in the parasite load. The literature has proven the effect of phenolic compounds as an anthelmintic in vivo, but no evidence for cyanogenic compounds, also present in all parts of the cassava plant, was found. A controlled in vitro bioassay was used to evaluate the aqueous extract of fresh cassava leaves. The efficiency parameter was the imobility of Haemonchus contortus larvae at stage L3, also used to evaluate commercial anthelmintics. Culture plates with 100 active L3 larvae per well were used, constituted a replicate of a total of three. It was placed in these wells, water extract of fresh cassava leaves (FCL) macerated in water, Ivomec® 0.01% (PCI) as positive control and distilled water as negative one (NCW). By considering the immobility of the larvae as a positive anthelmintic effect, the results showed that in NCW all larvae were mobile, while in PCI all 300 larvae were immobile. FCL produced a gradient of larval inactivation correlation (r2 0.996). The best fit equation was y = -33.39ln(x) + 40.517, a logarithmic one, which allowed to calculate the Lethal Concentration (CL) of 3. 44µg.CN-.ml, or 80.0 mg of fresh cassava leaves per milliliter, with performance equivalent to ivermectin. This concentration of free cyanide is compatible with safe consumption of fresh leaves by live weight of sheep. The exact amount of cassava roots, leaves or shoots consumed to provide an effective dose for controlling Haemonchus contort should be established in vivo. Although phenolic compounds must also be present in the extract, immobility was attributed to cyanogenic compounds since the correlation was proportional to the increase in cyanide concentration. It can be concluded that the consumption of fresh cassava leaves has potential as an anthelmintic agent to be evaluated in vivo by feeding sheep and goats. Local use could also add value to the production of fresh cassava leaves, with an average potential production of 2.5 t/ha, available throughout the year, with greater production at the beginning of cultivation and in the summer months. These leaves could be used after the roots harvesting or even in pruning for this purpose. Currently, this volume of good quality protein material is in the field, unused.

Effect of using different levels of cassava meal in a concentrate cassava peel diet on chemical composition, in vitro gas production, and rumen fermentation

This study was designed to evaluate effects of using different levels of cassava meal in a concentrate cassava peel diet on chemical composition, in vitro gas production (IVGP) and rumen fermentation. The treatments applied were: A=cassava peel (20%)+ cassava meal (70%)+cassava leaves (5%)+moringa leaves (5%); B=cassava peel (20%)+ cassava meal (60%)+cassava leaves (10%)+moringa leaves (10%); C=cassava peel (20%)+ cassava meal (50%)+cassava leaves (15%)+moringa leaves (15%) cassava meal; D=cassava peel (20%)+ cassava meal (40%)+cassava leaves (20%)+moringa leaves (20%); E=cassava peel (20%)+ cassava meal (30%)+cassava leaves (25%)+moringa leaves (25%) with 3 replications arranged in a Randomized Block Design (RBD). The result showed that the increasing levels of cassava meal in the ration significantly increased organic matter (OM) and nitrogen-free extract (NFE) content (P<0.05), but reduced crude protein (CP), ether extract (EE), crude fiber (CF), neutral detergent fiber (NDF), and acid detergent fiber (ADF) contents. Similarly, a significant increased (P<0.01) were found in values of cumulative in vitro gas production, dry matter and organic matter digestibility, but decreased NH3 concentration (P<0.05) due to the increased of cassava meal. It is concluded that increasing levels of cassava meal in concentrate has led to higher OM content of the ration and being available for rumen fermentation.

In Vitro Digesting Measurement of Cassava Leaves Using Gizzard Fluid and Chicken Duodenum

Cassava leaves are an alternative feed material that can be a feed material with sufficient protein content. The study aimed to determine the effect of gizzard and duodenal fluids on in vitro digesting measurements of cassava leaves. The study used a Complete Randomized Design (RAL) unidirectional pattern, Variance Analysis (ANOVA) with Duncan's Multiple Range Terst (DMRT) follow-up test using the SPSS application. The study used 4 treatments and 3 repeats: P0: No digestive fluids (controls), P1: Gizzard fluid, P2: Duodenal fluid, P3: A mixture of gizzard and duodenal fluids. The observed variables are the Dry Material Digest Coefficient (KCBK), the Organic Material Digest Coefficient (KCBO), and the Dissolved Protein Digest Coefficient (KCPT). The results of the study that the use of gizzard fluid in in vitro digestion measurements was better in increasing the value of in vitro digestant of cassava leaves. The use of gizzard fluid can increase the digestibility coefficient of organic matter (KCBO) by 21.2160% and the dissolved protein digest coefficient (KCPT) by 50.4620%.