Effective ways of keeping queen bees before and after instrumental insemination

The article presents theoretical and experimental data on the results of the instrumental insemination and alimentation of queen bees. Queen bees were bred and inseminated. It has been established that when using various methods of keeping queen bees, a dynamic pattern is observed when there is safekeeping of queen bees. So, in 2019, before insemination in all experimental groups, the death of 1 to 4 infertile queens was observed. The highest indicator was recorded in the first experimental group; this is due to the fact that there were not enough young bees in the transfer cells to fully feed the infertile queen. In 2020, when using various methods of keeping queen bees before and after insemination, a similar pattern is observed with respect to the safety of queen bees. In a comparative aspect, before and after insemination, a high death rate of queens was recorded in the I-experimental group - 4 pcs, respectively fetal - 16 pcs. Whereas in the II-experimental and IV-experimental groups, the safest keeping of 18-19 queens was recorded respectively. Whereas in 2021, the greatest safety of fetal queen bees was in the IV-experimental group - 90%, and in the II-experimental and III-experimental groups, 85% each in comparison with the I-experimental group - 75%. Consequently, when using the method of keeping infertile queens in the transfer cells with accompanying bees, 5 pieces each, there is a high death rate of queen bees. It was also confirmed that the method of keeping queens in a nursery frame without accompanying bees is less effective compared to the method of keeping queens in a nursery frame without accompanying bees. Also, when obtaining fetal queens by instrumental insemination, attention should be focused on phenotypic indicators when selecting infertile queens, since the size of the queen bee is directly correlated with the number of egg tubes and, accordingly, affects the egg production of the fetal queen bee.

Maternal Inheritance in Hybrids of Three Honey Bee Subspecies

The identification of honey bee (Apis mellifera) subspecies is often based on the measurements of workers’ fore-wings. The interpretation of the measurements can be difficult because the phenotype of workers is affected by both genetic and environmental factors. Moreover, it is not clear how the phenotype is affected by maternal inheritance. We have used the methodology of geometric morphometrics to verify if hybrids of honey bee subspecies and their backcrosses are more similar to either the father or mother colony. The comparison was based on fore-wing venation of three honey bee subspecies: A. m. carnica, A. m. caucasica, A. m. mellifera. First generation hybrids and backcrosses of those subspecies were obtained through instrumental insemination. Workers of the hybrids were compared with their parental colonies. The shape of wing venation was more similar to the maternal than to parental colony. This phenomenon was particularly visible in first generation of hybrids but it was also present in backcrosses. There were also symptoms of genetic dominance of some subspecies but this effect interacted with maternal inheritance and was difficult to interpret.

Multiple Virus Infections in Western Honeybee (Apis mellifera L.) Ejaculate Used for Instrumental Insemination

Instrumental insemination of Apis mellifera L. queens is a widely employed technique used in honeybee breeding that enables the effective control of mating. However, drone semen represents a potential source of honeybee viruses. In this study, 43 semen doses collected from apparently healthy drones, and consequently used in instrumental insemination, were analysed using PCR or RT-PCR to detect the presence of viral genome of 11 honeybee viruses. In 91% of samples, viral infection was detected. The survey revealed genomes of five viruses, namely Deformed wing virus (DWV), Acute bee paralysis virus (ABPV), Black queen cell virus (BQCV), Sacbrood virus (SBV), and A. mellifera filamentous virus (AmFV) in 84%, 19%, 14%, 2%, and 67% of samples, respectively. Single infection (30% of samples) as well as multiple infection (61% of samples) of two, three or four pathogens were also evaluated. To the best of our knowledge, this is the first study describing the presence of the BQCV and SBV genome sequence in drone ejaculate. Phylogenetic analysis of BQCV partial helicase gene sequence revealed the high similarity of nucleotide sequence of described Czech strains, which varied from 91.4% to 99.6%. The findings of our study indicate the possibility of venereal transmission of BQCV and SBV.

Major histocompatibility complex-linked social signalling affects female fertility

Genes of the major histocompatibility complex (MHC) have been shown to influence social signalling and mate preferences in many species, including humans. First observations suggest that MHC signalling may also affect female fertility. To test this hypothesis, we exposed 191 female horses ( Equus caballus ) to either an MHC-similar or an MHC-dissimilar stimulus male around the time of ovulation and conception. A within-subject experimental design controlled for non-MHC-linked male characteristics, and instrumental insemination with semen of other males ( n = 106) controlled for potential confounding effects of semen or embryo characteristics. We found that females were more likely to become pregnant if exposed to an MHC-dissimilar than to an MHC-similar male, while overall genetic distance to the stimulus males (based on microsatellite markers on 20 chromosomes) had no effect. Our results demonstrate that early pregnancy failures can be due to maternal life-history decisions (cryptic female choice) influenced by MHC-linked social signalling.

Differential performance of honey bee colonies selected for bee-pollen production through instrumental insemination and free-mating technique

ABSTRACT The use of bee-pollen as a nutritional supplement or as a production-enhancing agent in livestock has increased the demand for this product worldwide. Despite the current importance of this niche within the apiculture industry, few studies have addressed the pollen production. We tested the performance of free-mated (FM) and instrumentally inseminated queens (IQ) in order to establish the effect of different breeding systems on pollen production. The F1 generation of IQ queens produced 153.95±42.83g/day, showing a significant improvement on the pollen production (2.74 times) when compared to the parental generation (51.83±7.84g/day). The F1 generation of free-mated queens produced 100.07±8.23 g/day, which increased by 1.78 times when compared to the parental generation. Furthermore, we observed a statistically significant difference between the pollen production between colonies from the IQ and FM treatments. This study suggests that inseminated queens should be considered by beekeepers that aim to increase pollen production.