Upgrade of an integrated Langmuir probe system on the closed divertor target plates in the HL-2A tokamak

A newly designed divertor Langmuir probe diagnostic system has been installed in a rare closed divertor of the HL-2A tokamak and steadily operated for the study of divertor physics involved edge-localized mode (ELM) mitigation, detachment and redistribution of heat flux, etc. Two sets of probe arrays including 274 probe tips were placed at two ports (approximately 180° separated toroidally), and the spatial and temporal resolutions of this measurement system could reach 6 mm and 1 s, respectively. A novel design of the ceramic isolation ring can ensure reliable electrical insulation property between the graphite tip and the copper substrate plate where plasma impurities and the dust are deposited into the gaps for a long experimental time. Meanwhile, the condition monitoring and mode conversion between single and triple probe of the probe system could be conveniently implemented via a remote control station. The preliminary experimental result shows that the divertor Langmuir probe system is capable of measuring the high spatiotemporal parameters involved the plasma density, electron temperature, particle flux as well as heat flux during the ELMy H-mode discharges.

Development of a high-speed, phase-sensitive laser probe system for RF surface/bulk acoustic wave devices with an autofocus function for long time continuous operation

This paper describes the implementation of the autofocus function for the laser beam into the high-speed, phase-sensitive laser probe system for RF SAW/BAW devices. This implementation can compensate defocus caused during continuous measurements that take dozens of hours. After a brief explanation of the system used in this work, detailed discussion is given on an employed evaluation function indicating focus status, which is a key factor determining autofocus reliability. It is shown that the sum of energy of Laplacians is suitable as the evaluation function, which can be calculated by the image of the probing laser spot captured by a build-in CCD camera. Then, the implementation of the autofocus function into the current system is detailed. It is confirmed that this function can adjust the focus within almost ±20 μm defocus conditions. Finally, it is confirmed how the implemented autofocus function works effectively to keep just-in-focus under the disturbance.

Development of an In Vivo Probe to Track SARS-CoV-2 Infection in Rhesus Macaques

Infection with the novel coronavirus, SARS-CoV-2, results in pneumonia and other respiratory symptoms as well as pathologies at diverse anatomical sites. An outstanding question is whether these diverse pathologies are due to replication of the virus in these anatomical compartments and how and when the virus reaches those sites. To answer these outstanding questions and study the spatiotemporal dynamics of SARS-CoV-2 infection a method for tracking viral spread in vivo is needed. We developed a novel, fluorescently labeled, antibody-based in vivo probe system using the anti-spike monoclonal antibody CR3022 and demonstrated that it could successfully identify sites of SARS-CoV-2 infection in a rhesus macaque model of COVID-19. Our results showed that the fluorescent signal from our antibody-based probe could differentiate whole lungs of macaques infected for 9 days from those infected for 2 or 3 days. Additionally, the probe signal corroborated the frequency and density of infected cells in individual tissue blocks from infected macaques. These results provide proof of concept for the use of in vivo antibody-based probes to study SARS-CoV-2 infection dynamics in rhesus macaques.

LoopTag FRET Probe System for Multiplex qPCR Detection of Borrelia Species

Background: Laboratory diagnosis of Lyme borreliosis refers to some methods with known limitations. Molecular diagnostics using specific nucleic acid probes may overcome some of these limitations. Methods: We describe the novel reporter fluorescence real-time polymerase chain reaction (PCR) probe system LoopTag for detection of Borrelia species. Advantages of the LoopTag system include having cheap conventional fluorescence dyes, easy primer design, no restrictions for PCR product lengths, robustness, high sequence specificity, applicability for multiplex real-time PCRs, melting curve analysis (single nucleotide polymorphism analysis) over a large temperature range, high sensitivity, and easy adaptation of conventional PCRs. Results: Using the LoopTag probe system we were able to detect all nine tested European species belonging to the Borrelia burgdorferi (sensu lato) complex and differentiated them from relapsing fever Borrelia species. As few as 10 copies of Borrelia in one PCR reaction were detectable. Conclusion: We established a novel multiplex probe real-time PCR system, designated LoopTag, that is simple, robust, and incorporates melting curve analysis for the detection and in the differentiation of European species belonging to the Borrelia burgdorferi s.l. complex.

Estimation of probe measurements reliability in a supersonic flow of a four-component collisionless plasma

The aim of this work is to estimate the reliability of extracting the plasma electron density and temperature and ionic composition from the current-voltage (I-V) characteristic of an isolated probe system with cylindrical electrodes. An earlier proposed mathematical model of current collection by the probe system at positive bias potentials and an arbitrary ratio of the electrode areas is analyzed. The model is supplemented with a formula that determines, with an accuracy of several percent, the value of the bias potential at which the probe is under the plasma potential and the I-V characteristic splits into a transition and an electronic region. The analytical dependence of the bias potential on the plasma parameters and the ratio of the electrode areas made it possible to formalize the procedures for determining and assessing the reliability of the extracted plasma parameters using the regions of their strongest effect on the collected probe current. Parametric studies of the effect of the plasma parameters on the probe current were carried out for conditions close to measurements in the ionosphere. The paper demonstrates the feasibility of partitioning the sought-for plasma parameters into the regions of their strongest and weakest effect on the probe current in the range of the bias potentials considered. The problem of plasma parameter identification is formulated on the basis of a comparison of the probe current and the measured I-V characteristic in the L2 theoretical approximation. To each parameter there corresponds an objective function of its own, which differs in the domain of definition and the ratio of the electrode areas used in I-V characteristic measurements. Based on this formulation of the inverse problem in L2, estimates of the reliability of identification of the parameters of a plasma with two ion species are obtained depending on the errors of the model and probe measurements. The results obtained may be used in ionospheric plasma diagnostics.