Keberadaan Mikoriza Arbuskular pada Lokasi Pertanaman Jarak Pagar (Jatropha curcas L.) di Lembah Palu

Plant rhizosphere has various types of microorganisms, including Arbuscular Mycorrhizae Fungi (AMF). Each ecosystem has different species and densities of AMF. For further use, study the potency of indigenous AMF is necessary. This research was conducted to know the existence and potency of indigenous AMF of soil where physic nuts grow on dry land of Palu Valley at Poboya, Palu, Central Sulawesi. Soil samples were collected, and then observed under microscope. The steps to study the potency of AMF were counting the propagules with Most Probable Number (MPN) method, spora trapping, identifying the types of spore, and single spore culture. The result showed that the number of infective AMF propagules from cultivated soil was 1117 microorganisms/g soil and from of natural soil was 711 microorganisms/g soil; and indigenous AMF from the soil where physic nut grown at Lembah Palu were dominated by Glomus sp.

PENGEMBANGAN TEKNOLOGI SELEKSI IN VITRO BERULANG (REPEAT CYCLING–IN VITRO SELECTION) TOLERAN STRES KEKERINGAN UNTUK PERBAIKAN MUTU GENETIK DAN PRODUKSI TANAMAN KEDELAI BERMIKORIZA

Every plant rhizospheres in any ecosystem there are various living microorganisms including Arbuscular Mycorrhizae Fungi (AMF). An isolation and characterization is required to investigate the species or type of the AMF. This research was aimed at studying the isolation and characterization of AMF sporulation in soybean rhizospheres in Jambi Province. The results of evaluation on soil samples before trapping showed that there are spores from three genus of AMF twelve types Glomus , two types Acaulospora and one type of Enthrophospora. Following single spore culture in soybean rhizosphere, 5 spore types were obtained: Glomus sp-1, Glomus sp-4, Glomus sp-7, Glomus sp-8 Glomus sp-10.

THE HETEROTHALLISM OF PANAEOLUS SUBBALTEATUS BERK., A SCLEROTIUM-PRODUCING AGARIC

Panaeolus subbalteatus, an uncommon coprophilous agaric, has been grown in single-spore culture on malt-extract agar.The fungus is heterothallic, exhibiting four sexual groups and a remarkable regularity in its pairing reactions.Both haplophytes and diplophytes produce sclerotia of a striking greenish-blue color. These sclerotia are capable of producing mycelium, even after they have been dried for some weeks, but do not give rise to fruit bodies.The haplophyte is distinguishable macroscopically as well as microscopically from the diplophyte.The mycelia do not exhibit the phenomenon of mutual aversion or barrage.