Rider and Sunflower optimization-driven neural network for image classification

Image classification is the classical issue in computer vision, machine learning, and image processing. The image classification is measured by differentiating the image into the prescribed category based on the content of the vision. In this paper, a novel classifier named RideSFO-NN is developed for image classification. The proposed method performs the image classification by undergoing two steps, namely feature extraction and classification. Initially, the images from various sources are provided to the proposed Weighted Shape-Size Pattern Spectra for pattern analysis. From the pattern analysis, the significant features are obtained for the classification. Here, the proposed Weighted Shape-Size Pattern Spectra is designed by modifying the gray-scale decomposition with Weight-Shape decomposition. Then, the classification is done based on Neural Network (NN) classifier, which is trained using an optimization approach. The optimization will be done by the proposed Ride Sunflower optimization (RideSFO) algorithm, which is the integration of Rider optimization algorithm (ROA), and Sunflower optimization algorithm (SFO). Finally, the image classification performance is evaluated using RideSFO-NN based on sensitivity, specificity, and accuracy. The developed RideSFO-NN method achieves the maximal accuracy of 94%, maximal sensitivity of 93.87%, and maximal specificity of 90.52% based on K-Fold.

Shape decomposition algorithms for laser capture microdissection

Background In the context of biomarker discovery and molecular characterization of diseases, laser capture microdissection is a highly effective approach to extract disease-specific regions from complex, heterogeneous tissue samples. For the extraction to be successful, these regions have to satisfy certain constraints in size and shape and thus have to be decomposed into feasible fragments. Results We model this problem of constrained shape decomposition as the computation of optimal feasible decompositions of simple polygons. We use a skeleton-based approach and present an algorithmic framework that allows the implementation of various feasibility criteria as well as optimization goals. Motivated by our application, we consider different constraints and examine the resulting fragmentations. We evaluate our algorithm on lung tissue samples in comparison to a heuristic decomposition approach. Our method achieved a success rate of over 95% in the microdissection and tissue yield was increased by 10–30%. Conclusion We present a novel approach for constrained shape decomposition by demonstrating its advantages for the application in the microdissection of tissue samples. In comparison to the previous decomposition approach, the proposed method considerably increases the amount of successfully dissected tissue.

DeepACSON automated segmentation of white matter in 3D electron microscopy

Tracing the entirety of ultrastructures in large three-dimensional electron microscopy (3D-EM) images of the brain tissue requires automated segmentation techniques. Current segmentation techniques use deep convolutional neural networks (DCNNs) and rely on high-contrast cellular membranes and high-resolution EM volumes. On the other hand, segmenting low-resolution, large EM volumes requires methods to account for severe membrane discontinuities inescapable. Therefore, we developed DeepACSON, which performs DCNN-based semantic segmentation and shape-decomposition-based instance segmentation. DeepACSON instance segmentation uses the tubularity of myelinated axons and decomposes under-segmented myelinated axons into their constituent axons. We applied DeepACSON to ten EM volumes of rats after sham-operation or traumatic brain injury, segmenting hundreds of thousands of long-span myelinated axons, thousands of cell nuclei, and millions of mitochondria with excellent evaluation scores. DeepACSON quantified the morphology and spatial aspects of white matter ultrastructures, capturing nanoscopic morphological alterations five months after the injury.

DeepACSON: Automated Segmentation of White Matter in 3D Electron Microscopy

ABSTRACTAutomated segmentation techniques are essential to tracing the entirety of ultrastructures in large three-dimensional electron microscopy (3D-EM) images of the brain tissue. Current automated techniques use deep convolutional neural networks (DCNNs) and rely on high-contrast cellular membranes to trace a small number of neuronal processes in very high-resolution EM datasets. We developed DeepACSON to segment large field-of-view, low-resolution 3D-EM datasets of white matter where tens of thousands of myelinated axons traverse the tissue. DeepACSON performs DCNN-based semantic segmentation and shape decomposition-based instance segmentation. With its top-down design, DeepACSON manages to account for severe membrane discontinuities inescapable with the low-resolution imaging. In particular, the instance segmentation of DeepACSON uses the tubularity of myelinated axons, decomposing an under-segmented myelinated axon into its constituent axons. We applied DeepACSON to ten serial block-face scanning electron microscopy datasets of rats after sham-operation or traumatic brain injury, segmenting hundreds of thousands of long-span myelinated axons, thousands of cell nuclei, and millions of mitochondria with excellent evaluation scores. DeepACSON quantified the morphology and spatial aspects of white matter ultrastructures, capturing nanoscopic morphological alterations five months after the injury.