shock gene
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Author(s):  
Ram Hari Dahal ◽  
Dhiraj Kumar Chaudhary ◽  
Dong-Uk Kim ◽  
Jaisoo Kim

2019 ◽  
Author(s):  
Akram Mohammed ◽  
Yan Cui ◽  
Valeria R. Mas ◽  
Rishikesan Kamaleswaran

AbstractSeptic shock is a severe health condition caused by uncontrolled sepsis. Advancements in the high-throughput sequencing techniques have risen the number of potential genetic biomarkers under review. Multiple genetic markers and functional pathways play a part in the development and progression of pediatric septic shock. Fifty-four differentially expressed pediatric septic shock gene biomarkers were identified using gene expression data from 181 pediatric intensive care unit (PICU) patients within the first 24 hours of admission. The gene expression signatures discovered showed discriminatory power between pediatric septic shock survivors and nonsurvivors types. Using functional enrichment analysis of differentially expressed genes (DEGs), the known genes and pathways in septic shock were validated, and unexplored septic shock-related genes and functional groups were identified. Septic shock survivors were distinguished from septic shock non-survivors by differential expression of genes involved in the immune response, chemokine-mediated signaling, neutrophil chemotaxis, and chemokine activity. The identification of the septic shock gene biomarkers may facilitate in septic shock diagnosis, treatment, and prognosis.


2019 ◽  
Vol 20 (3) ◽  
pp. 457 ◽  
Author(s):  
Anna Giuliodori ◽  
Attilio Fabbretti ◽  
Claudio Gualerzi

In Escherichia coli, the mRNA transcribed from the main cold-shock gene cspA is a thermosensor, which at low temperature adopts a conformation particularly suitable for translation in the cold. Unlike cspA, its paralogue cspD is expressed only at 37 °C, is toxic so cannot be hyper-expressed in E. coli and is poorly translated in vitro, especially at low temperature. In this work, chimeric mRNAs consisting of different segments of cspA and cspD were constructed to determine if parts of cspA could confer cold-responsive properties to cspD to improve its expression. The activities of these chimeric mRNAs in translation and in partial steps of translation initiation such as formation of 30S initiation complexes and 50S subunits docking to 30S complexes to yield 70S initiation complexes were analyzed. We show that the 5′ untranslated region (5′UTR) of cspA mRNA is sufficient to improve the translation of cspD mRNA at 37 °C whereas both the 5′UTR and the region immediately downstream the cspA mRNA initiation triplet are essential for translation at low temperature. Furthermore, the translational apparatus of cold-stressed cells contains trans-active elements targeting both 5′UTR and downstream regions of cspA mRNA, thereby improving translation of specific chimeric constructs at both 15 and 37 °C.


2018 ◽  
Vol 506 (4) ◽  
pp. 799-804 ◽  
Author(s):  
Ryan Oliverio ◽  
Peter Nguyen ◽  
Brianna Kdeiss ◽  
Sara Ord ◽  
Amanda J. Daniels ◽  
...  

2018 ◽  
Vol 140 ◽  
pp. 444-454 ◽  
Author(s):  
Silvia Franzellitti ◽  
Valentina Airi ◽  
Diana Calbucci ◽  
Erik Caroselli ◽  
Fiorella Prada ◽  
...  

2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
Jason W. Tresser ◽  
Megan Bliss ◽  
Hailey Mayweather ◽  
Emerson Rustand ◽  
Alex Sears ◽  
...  

2017 ◽  
Vol 217 (4) ◽  
pp. 1696-1711 ◽  
Author(s):  
Yucheng Sun ◽  
Huijuan Guo ◽  
Erliang Yuan ◽  
Feng Ge

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