Chromosome-Level Genome Assembly of a Human Fungal Pathogen Reveals Synteny among Geographically Distinct Species

Histoplasma species are dimorphic fungi causing significant morbidity and mortality worldwide. These fungi grow as mold in the soil and as budding yeast within the human host. Histoplasma can be isolated from soil in diverse regions, including North America, South America, Africa, and Europe.

Worldwide Traceability of Antibiotic Residues from Livestock in Wastewater and Soil: A Systematic Review

The use of antibiotics in animal production are widely used for disease treatment, health protection, and as growth promoters. Common antibiotics used in veterinary medicine are excreted and eliminated through the sewage system, contaminating water and soil with negative effects on agricultural activities. This systematic review focuses on the trend of research works on antibiotic residues, evaluating antibiotics used in livestock production and their excretion in animal products and in environmental matrices such as water and soil. Our database was composed of 165 articles, reporting the concentration of antibiotic residues found in the environment, livestock (cow, sheep, pig, horse, chicken, rabbit, goat), aquatic and terrestrial animal tissues, animal products (milk and eggs), wastewater, and soil. The documents were obtained from Asia, Africa, North America, South America, Europe, and Oceania. A descriptive analysis of antibiotic residues found worldwide was analyzed according to each of the variables used such as antibiotic family, name, concentration (% and mg/kg or ppm), and country and continent where the residue was found. The descriptive analysis was carried out using the “describe” function of psych package and pirate plots were drawn. According to our study, the main antibiotics used worldwide in animal production are sulfonamides, tetracyclines, quinolones, penicillin, and cephalosporins. At present, despite the trends of increased regulations on the use of antibiotics worldwide, antibiotics are still utilized in food animal production, and are present in water and soil, then, there is still the misuse of antibiotics in many countries. We need to become aware that antibiotic contamination is a global problem, and we are challenged to reduce and improve their use.

Potential distribution of Croton guatemalensis: a model with reproductive biology data

Background: The inclusion of information on the phenology of any given species can significantly improve the resulting of potential distribution models. Scientific literature does not provide up-to-date information on the abiotic and biotic factors that determine the distribution of Croton guatemalensis, a species native to communities in south Mexico. For the first time, the potential distribution of C. guatemalensis was determined using a model which includes reproductive biology data. Questions: Which bioclimatic and climatic variables most contribute to the distribution of C. guatemalensis? Does reproductive biology data contribute significantly to the prediction of the species distribution? Studied species/Mathematical model: Croton guatemalensis/ Maximum Entropy Modeling Study area and dates: Chiapas, Mexico, January - December 2020. Methods: The MaxEnt 4.4.4 algorithm was used, incorporating 16 variables, including bioclimatic, climatic and elevation. In addition, a habitat suitability layer was built. Results: The model presented a precision of AUC = 0.964 ± 0.004. Eight variables contributed to explain 86.5 % of the potential distribution of the species. According to their contribution to the model, the most important were the seasonality of precipitation, habitat suitability, elevation and April solar radiation. The species was found in the physiographic regions Central America South Mountain Range Subprovince, Central Depression of Chiapas Discontinuity, and Altos de Chiapas Subprovince. Conclusions: The inclusion reproductive biology data of C. guatemalensis contributed to improve the model. This information allows the development of more effective management and conservation plans by identifying the precise regions in which the species is found.

Pan-Piscine Orthoreovirus (PRV) Detection Using Reverse Transcription Quantitative PCR

Piscine orthoreovirus (PRV) infects farmed and wild salmon and trout species in North America, South America, Europe, and East Asia. PRV groups into three distinct genotypes (PRV-1, PRV-2, and PRV-3) that can vary in distribution, host specificity, and/or disease potential. Detection of the virus is currently restricted to genotype specific assays such that surveillance programs require the use of three assays to ensure universal detection of PRV. Consequently, herein, we developed, optimized, and validated a real-time reverse transcription quantitative PCR assay (RT-qPCR) that can detect all known PRV genotypes with high sensitivity and specificity. Targeting a conserved region at the 5′ terminus of the M2 segment, the pan-PRV assay reliably detected all PRV genotypes with as few as five copies of RNA. The assay exclusively amplifies PRV and does not cross-react with other salmonid viruses or salmonid host genomes and can be performed as either a one- or two-step RT-qPCR. The assay is highly reproducible and robust, showing 100% agreement in test results from an inter-laboratory comparison between two laboratories in two countries. Overall, as the assay provides a single test to achieve highly sensitive pan-specific PRV detection, it is suitable for research, diagnostic, and surveillance purposes.

Utilization of Maternal Health Care Among Immigrant Mothers in New York City, 2016–2018

Immigrant women represent half of New York City (NYC) births, and some immigrant groups have elevated risk for poor maternal health outcomes. Disparities in health care utilization across the maternity care spectrum may contribute to differential maternal health outcomes. Data on immigrant maternal health utilization are under-explored in the literature. We conducted a cross-sectional analysis of the population-based NYC Pregnancy Risk Assessment Monitoring System survey, using 2016–2018 data linked to birth certificate variables, to explore self-reported utilization of preconception, prenatal, and postpartum health care and potential explanatory pathways. We stratified results by maternal nativity and, for immigrants, by years living in the US; geographic region of origin; and country of origin income grouping. Among immigrant women, 43% did not visit a health care provider in the year before pregnancy, compared to 27% of US-born women (risk difference [RD] = 0.16, 95% CI [0.13, 0.20]), 64% had no dental cleaning during pregnancy compared to 49% of US-born women (RD = 0.15, 95% CI [0.11, 0.18]), and 11% lost health insurance postpartum compared to 1% of US-born women (RD = 0.10, 95% CI [0.08, 0.11]). The largest disparities were among recent arrivals to the US and immigrants from countries in Central America, South America, South Asia, and sub-Saharan Africa. Utilization differences were partially explained by insurance type, paternal nativity, maternal education, and race and ethnicity. Disparities may be reduced by collaborating with community-based organizations in immigrant communities on strategies to improve utilization and by expanding health care access and eligibility for public health insurance coverage before and after pregnancy.

Studies on the Systematics and Anatomy of New Zealand Earthworms

<p>The study of New Zealand earthworms has been extensive, but has been confined principally to the systematics of the group. Only one family of the Oligochaeta, the Megascolecidae, is represented in the endemic fauna, but within this family, over eighty species, belonging to seventeen genera, have been recorded and described. Apart from the Megascolecidae, certain species, lumbricids, worldwide in their distribution, are present and are regarded as having been introduced through the agency of man. The family Megascolecidae is confined almost entirely to the Southern hemisphere, and the southern regions of the Northern hemisphere, and within these regions, the greatest number of species occur in New Zealand, South America, South Africa, and Australia. When the distribution of the Megascolecidae became known, in the late nineteenth century, its sporadic nature evoked a great deal of interest among zoo-geographers, since earthworms, being terrestrial, and unable to tolerate immersion in salt water, form an ideal basis for the consideration of dispersal problems among terrestrial animals as a whole. The interest thus aroused in the Megascolecidae led to much work on the group in New Zealand. Michaelsen (1913 (b)) accounts for the predominance of the Megascolecidae in the southern continental areas by postulating that originally the family had a wide distribution in the nothern and southern continents, and that other families (e.g. the Glossoscolecidae), evolved more recently in the northern continents, have gradually superseded the Megascolecidae in all but the most remote regions of their original area of distribution. Matthew (1915) came to a similar conclusion in regard to the origin of present southern faunas in the course of his work on the distribution and evolution of the Mammalia. Evidence in favour of Michaelsen's conclusions can also be derived from the distribution of slugs, spiders, Collembola, Coleoptera, littoral Echinoderms, Polychaeta and Brachiopoda in the southern land masses.</p>

Studies on the Systematics and Anatomy of New Zealand Earthworms

<p>The study of New Zealand earthworms has been extensive, but has been confined principally to the systematics of the group. Only one family of the Oligochaeta, the Megascolecidae, is represented in the endemic fauna, but within this family, over eighty species, belonging to seventeen genera, have been recorded and described. Apart from the Megascolecidae, certain species, lumbricids, worldwide in their distribution, are present and are regarded as having been introduced through the agency of man. The family Megascolecidae is confined almost entirely to the Southern hemisphere, and the southern regions of the Northern hemisphere, and within these regions, the greatest number of species occur in New Zealand, South America, South Africa, and Australia. When the distribution of the Megascolecidae became known, in the late nineteenth century, its sporadic nature evoked a great deal of interest among zoo-geographers, since earthworms, being terrestrial, and unable to tolerate immersion in salt water, form an ideal basis for the consideration of dispersal problems among terrestrial animals as a whole. The interest thus aroused in the Megascolecidae led to much work on the group in New Zealand. Michaelsen (1913 (b)) accounts for the predominance of the Megascolecidae in the southern continental areas by postulating that originally the family had a wide distribution in the nothern and southern continents, and that other families (e.g. the Glossoscolecidae), evolved more recently in the northern continents, have gradually superseded the Megascolecidae in all but the most remote regions of their original area of distribution. Matthew (1915) came to a similar conclusion in regard to the origin of present southern faunas in the course of his work on the distribution and evolution of the Mammalia. Evidence in favour of Michaelsen's conclusions can also be derived from the distribution of slugs, spiders, Collembola, Coleoptera, littoral Echinoderms, Polychaeta and Brachiopoda in the southern land masses.</p>

A plain language summary of how well the single-dose Janssen vaccine works and how safe it is

This is a summary of a publication about the ENSEMBLE trial of the Janssen Ad26.COV2.S vaccine against COVID-19, which was published in the New England Journal of Medicine in April 2021. The ENSEMBLE study started in September 2020 and is still ongoing. The study compared the effectiveness of the vaccine to a placebo in 43,783 adults from Latin America, South Africa, and the United States. Of those, 19,630 got a single dose of the vaccine. Compared to the placebo, the vaccine prevented: 66.9% of moderate to severe–critical COVID-19 cases after 14 days 66.1% of moderate to severe–critical COVID-19 cases after 28 days 85.4% of severe COVID-19 cases after 28 days 100% of people with severe COVID-19 from needing to go to hospital for treatment None of the vaccinated participants died from COVID-19. There were 5 people who got the placebo who died from COVID-19. The vaccine was similarly effective in people from all age groups and different countries, including South Africa, where most cases were caused by the beta variant of the virus that originated there. The people in the study who got the vaccine who went on to get COVID-19 generally had milder and fewer symptoms than those who got the placebo. In most people, the vaccine started working after about 2 weeks. After receiving the vaccine, some people experienced pain at the injection site, headache, tiredness, muscle pain, and nausea. In most cases, these were mild and went away within a few days. Serious side effects were very rare. Blood clots, seizures, and tinnitus were very rare but were more common in the people who got the vaccine than in those who got the placebo. At the time of the study, it was not clear if these were caused by the vaccine or not. ClinicalTrials.gov NCT number: NCT04505722 .

New Record of the Bottle Liverworts Sphaerocarpos Donnellii Aust. for Iraq and Middle East Countries

The Order Sphaerocarpales of the bottle liverwort consists of five genera, among which Sphaerocarpos in turn consists of 8-9 species. The genus is nearly worldwide distributed, but disjunct, sporadic, and localized throughout the range in North America, South America, Europe, Africa, and Australia. According to the published checklist, only two species of Sphaerocarpos were recorded in the middle east countries. These are S. texanus and S. michelii, both were found in Turkey, while only the latter was found in Iraq. By the current study, an additional species, S. donnellii, will be added to the byroflora of the Middle East (south west Asia) region. A specimen of this species was found in Mosul city, Nineveh province, Iraq, grown on loamy soil in a house garden.

Transition to Open Science

Many initiatives addressing different types of problems of the practice of science and research have been described or cited in this book. Some were one-issue local actions, some took a broader approach at the national and some at EU level. Some stayed on, others faded after a few years. Many of the issues addressed by these movements and initiatives were part of the system of science and appeared to be systemically interdependent. This is how they converged and precipitated in the movement of Open Science, somewhere at the beginning of the second decade of this century. I discuss the major move that was made since 2015 in the EU to embrace the Open Science practice as the way science and research are being done in Europe. This elicited tensions at first foremost relate to uncertainty regarding scholarly publishing, of how and where we publish open access. But also, with respect to what immediate sharing of data and results in daily practice of researchers means, how we value and give credit for papers and published data sets. It thus poses the question of how, if at all, we must compare incomparable academic work, how we get credit and build reputations in this new open practice of science. It is indeed believed that Open Science with its practice of responsible science will be a major contribution to address the dominant problems in science that we have analysed thus far, or at least will help to mitigate them. Open Science holds a promise to take science to the next phase as outlined in the previous chapters. That is not a romantic naive longing for the science that once was. It will be a truly novel way, but realistic way of doing scientific inquiry according to the pragmatic narrative pointed out.The Transition to Open Science as can be anticipated from the analyses above will not be trivial. The recent discussions have already shown that the transition to Open Science, even between EU member states, is a very different thing because of specific national, societal and academic contexts.I will conclude this chapter reporting some of my first-hand experiences, in Brussels and during visits to several EU member states in the course of a Mutual Learning Exercise, but also encounters in North America, South East Asia and South Africa where we in the past years have discussed Open Science. Although we know science and scholarship have many forms and flavours and that wherever you go, there is not one scientific community. For me discussing the Transition to Open Science in the past four years was really a Learning Exercise, an amazing, mostly encouraging, but many times quite shocking, even saddening adventure.