Therapeutic Management of Pyometra with Methylergometrine and Cabergoline in Bitches - An Alternative Approach

Background: Canine pyometra is a common reproductive disorder of sexually intact bitches characterized by accumulation of pus in the uterine lumen and it is always associated with vagaries of clinical and pathological manifestations of multisystemic origin. Methods: The present study involved twenty three bitches of 8- 13 years of age with pyometra. Diagnosis of pyometra was performed based on the anamnesis, ultrasound examination, hematoglocial and biochemical changes. As an alternate to conventional prostaglandin protocol, Tab. Methylergometrine 0.125 mg/dog (Methergine, Novartis) BID and Tab. Cabergoline @ 10 µg/kg body weight (Cabgolin, Sun Pharma) SID and Tab. Amoxicillin- Clavulinic acid @ 10 mg/kg body weight (Augmentin, Glaxo Smithkline) BID was advised for two weeks. In addition to that supportive fluid therapy and Inj. Pantoprazole @ 1 mg/kg body weight was also administered prior to initiation of treatment protocol. There was a significant clinical recovery in terms of physiological, hematological and biochemical parameters. Ultrasound examination on Day 0, 7, 9 and Day 14 of treatment revealed reduction in the diameter of uterine sacculations. All the bitches recovered after 10 to 15 days with complete evacuation of pus, with no recurrence. Result: Our investigation revealed that this alternative protocol along with antibiotics and supportive fluid therapy can be a better alternative to young dogs to preserve the reproductive capability and also in geriatric dogs which are unfit for traditional ovariohysterectomy.

Differences in gene expression and variable splicing events of ovaries between large and small litter size in Chinese Xiang pigs

Background Although lots of quantitative trait loci (QTLs) and genes present roles in litter size of some breeds, the information might not make it clear for the huge diversity of reproductive capability in pig breeds. To elucidate the inherent mechanisms of heterogeneity of reproductive capability in litter size of Xiang pig, we performed transcriptome analysis for the expression profile in ovaries using RNA-seq method. Results We identified 1,419 up-regulated and 1,376 down-regulated genes in Xiang pigs with large litter size. Among them, 1,010 differentially expressed genes (DEGs) were differently spliced between two groups with large or small litter sizes. Based on GO and KEGG analysis, numerous members of genes were gathered in ovarian steroidogenesis, steroid biosynthesis, oocyte maturation and reproduction processes. Conclusions Combined with gene biological function, twelve genes were found out that might be related with the reproductive capability of Xiang pig, of which, eleven genes were recognized as hub genes. These genes may play a role in promoting litter size by elevating steroid and peptide hormones supply through the ovary and facilitating the processes of ovulation and in vivo fertilization.

Multi Species Analyses Reveal Testicular T3 Metabolism and Signalling as a Target of Environmental Pesticides

Thyroid hormones (THs) regulate many biological processes in vertebrates, including reproduction. Testicular somatic and germ cells are equipped with the arrays of enzymes (deiodinases), transporters, and receptors necessary to locally maintain the optimal level of THs and their signalling, needed for their functions and spermatogenesis. Pesticides, as chlorpyrifos (CPF) and ethylene thiourea (ETU), impair the function of thyroid and testis, affecting male fertility. However, their ability to disarrange testicular T3 (t-T3) metabolism and signalling is poorly considered. Here, a multi-species analysis involving zebrafish and mouse suggests the damage of t-T3 metabolism and signalling as a mechanism of gonadic toxicity of low-doses CPF and ETU. Indeed, the developmental exposure to both compounds reduces Dio2 transcript in both models, as well as in ex-vivo cultures of murine seminiferous tubules, and it is linked to alteration of steroidogenesis and germ cell differentiation. A major impact on spermatogonia was confirmed molecularly by the expression of their markers and morphologically evidenced in zebrafish. The results reveal that in the adopted models, exposure to both pesticides alters the t-T3 metabolism and signalling, affecting the reproductive capability. Our data, together with previous reports suggest zebrafish as an evaluable model in assessing the action of compounds impairing locally T3 signalling.

AgNPs reduce reproductive capability of female mouse for their toxic effects on mouse early embryo development

Silver nanoparticles (AgNPs) are widely applied in the field of personal protection for their powerful toxic effects on cells, and recently, a new type of vaginal gel with AgNPs is used to protect the female reproductive tract from microbes and viruses. However, a high risk of AgNPs to the fetus and the underlying mechanism of AgNPs to interfere in embryo development still remain unclear. Thus, this study investigated the impact of two drugs of vaginal gel with AgNPs on reproductive capability of the female mouse by animal experiment. Then, kinetics of AgNPs affecting embryo development was investigated by in vitro embryos culturing, and cell membrane potential (CMP) of zygotes was analyzed by DiBAC4(3) staining. Results indicated that one of the drugs of vaginal gel certainly injured embryo development in spite of no apparent histological change found in ovaries and uteruses of drug-treated mice. In vitro embryo culturing discovered that the toxic effect of AgNPs on embryo development presented particle sizes and dose dependent, and AgNP treatment could rapidly trigger depolarization of the cell membrane of zygotes. Moreover, AgNPs changed the gene expression pattern of Oct-4 and Cdx2 in blastocysts. All these findings suggest that AgNPs can interfere with normal cellular status including cell membrane potential, which has not been noticed in previous studies on the impact of AgNPs on mammalian embryos. Thus, findings of this study alarm us the risk of applying vaginal gel with AgNPs in individual caring and protection of the female reproductive system.

Gene-drive suppression of mosquito populations in large cages as a bridge between lab and field

CRISPR-based gene-drives targeting the gene doublesex in the malaria vector Anopheles gambiae effectively suppressed the reproductive capability of mosquito populations reared in small laboratory cages. To bridge the gap between laboratory and the field, this gene-drive technology must be challenged with vector ecology.Here we report the suppressive activity of the gene-drive in age-structured An. gambiae populations in large indoor cages that permit complex feeding and reproductive behaviours.The gene-drive element spreads rapidly through the populations, fully supresses the population within one year and without selecting for resistance to the gene drive. Approximate Bayesian computation allowed retrospective inference of life-history parameters from the large cages and a more accurate prediction of gene-drive behaviour under more ecologically-relevant settings.Generating data to bridge laboratory and field studies for invasive technologies is challenging. Our study represents a paradigm for the stepwise and sound development of vector control tools based on gene-drive.

LAPAROSCOPIC MANAGEMENT OF AN ECTOPIC PREGNANCY IN A PREVIOUS CAESAREAN SECTION SCAR: A CASE REPORT

Caesarean scar ectopic is one of the rarest forms of ectopic pregnancy [3]. The diagnosis and treatment of it is challenging. It is important to diagnose the condition as early as possible in order to administer appropriate, timely treatment and to avoid complications. A rare case of laparoscopic management of an ectopic pregnancy in a previous Caesarean section scar is reported here. Laparoscopy enabled the successful treatment of an unruptured ectopic pregnancy in a previous Caesarean scar and made it possible to preserve the patient’s reproductive capability. In this report we present a case of 30 year old gravida 2, para 1, living 1 with cesarean scar ectopic pregnancy managed laparoscopically.

Efficiency of the substrates application in the hazelnut (Corylus maxima Mill.) mother garden of the vegetative reproduction

The author presents the results of the researches carried out during the seven year (2013-2019) period of exploiting the mother garden of the hazelnut vegetative reproduction in the vertical way (the planting plan is 1.5 x 0.3 m) with the different substrates application. The studied cultivars were Sviatkovyi, Dolynskyi, Koronchatyi and Darunok Yunnatam. The analysis of their influence on the reproductive capability of the obtained layers was conducted as well as on their quality. The data of the two-factor experiment disperse analysis show that the substrate effect on the standard layers yield is the greatest one, of cv somewhat less and of their interaction still less. The economic analysis of the data attained when using different substrates to hill up layers in the mother garden as well as of the multifunctional biological preparation Extracon makes it possible to determine the expediency and rationality of their usage. The yield of layers, including standard ones, their price cost, profit were analysed as well as the profitableness level. The estimation of the layers growing economic effectivity depending on a substrate has indicated that the total cost sum depends on its type and amount and quality of the obtained layers. The maximum price cost of the standard layers of the investigated varieties is in the control (soil –11,3-12,5 ₴) whereas when applying substrates and Extracon 5,0-7,1 ₴. The price and total cost of the standard layers depends considerably on their number. The use of the substrates and the biopreparation Extracon contributes to the profit increase by 2,3-7,9 times as compared to the control treatment. The expediency of the substrates usage has been established as well as the ability of the layers of the studied cultivars to be rooted. The most effective applied substrates have been determined. Thus the maximum economic effect has been achieved when using the mineralized peat as well as sawdust with Extracon. This enables to repay the invested costs during one exploitation year that is in 3 years after the mother garden establishment because it takes two years to establish a high quality mother garden. Besides, for the effective layers rooting the soil humidity must be kept within 70 % of the lowest field water capacity, especially during the root formation period (the second half of Auqust-September).

The Effects of Postharvest Treatments and Sunlight Exposure on the Reproductive Capability and Viability of Phyllosticta citricarpa in Citrus Black Spot Fruit Lesions

Citrus black spot (CBS) is caused by Phyllosticta citricarpa, which is classified as a quarantine organism in certain countries whose concerns are that CBS-infected fruit may be a pathway for introduction of the pathogen. This study evaluated the reproductive capability and viability of P. citricarpa under simulated conditions in which the whole fruit, peel segments, or citrus pulp with CBS lesions were discarded. Naturally infected ‘Midknight’ Valencia orange and ‘Eureka’ lemon fruit, either treated using standard postharvest sanitation, fungicide, and wax coating treatments or untreated, were placed into cold storage for 5 weeks (oranges at 4 °C and lemons at 7 °C). Thereafter, treated and untreated fruit were incubated for a further 2 weeks at conditions conducive for CBS symptom expression and formation of pycnidia. The ability of pycnidia to secrete viable pycnidiospores after whole fruit and peel segments or peel pieces from citrus pulp were exposed to sunlight at warm temperatures (±28 °C) and ±75% relative humidity levels was then investigated. The combination of postharvest treatments and cold storage effectively controlled CBS latent infections (>83.6% control) and pycnidium formation (<1.4% of lesions formed pycnidia), and the wax coating completely inhibited pycnidiospore release in fruit and peel segments. Pycnidiospores were secreted only from lesions on untreated fruit and peel segments and at low levels (4.3–8.6%) from peel pieces from pulped treated fruit. However, spore release rapidly declined when exposed to sunlight conditions (1.4% and 0% after 2 and 3 days, respectively). The generally poor reproductive ability and viability of CBS fruit lesions on harvested fruit, particularly when exposed to sunlight conditions, supports the conclusion that citrus fruit without leaves is not an epidemiologically significant pathway for the entry, establishment, and spread of P. citricarpa.

AN AYURVEDIC OVERVIEW OF RAKTAPRADAR- A CONCEPTUAL APPROACH

Reproductive capability in a young woman begins at the point of menarche, which is the beginning of cyclic uterine bleeding in the anatomically and physiologically normal female. Menarche marks the beginning of an important stage in a young woman’s physical reproductive maturation and development. Even before the onset of this entirely natural but potentially disturbing function, a young woman’s early psychological reactions to menstruation, and probably also her lifelong view, can be influenced by the accuracy of her information and the degree of empathy with which this knowledge has been conveyed to her. Many women, perhaps appropriately, conclude that any departure from their personal menstrual experience is abnormal, and they will seek treatment for these departures. Conversely, some women accept or perhaps ignore even significant variations in their menstrual function, sometimes to the extent that serious health impairment occurs (e.g., severe iron-deficiency anemia). Therefore in this study an effort has been put forth to make a conceptual study covering almost all the aspect of Raktapradar as per Ayurvedic and modern.