ABSTRACT Background: Tuberculosis (TB) is a national health problem both in the world and in Indonesia. Diabetes mellitus (DM) is a comorbid disease that contributes to 15% of TB cases in the world. In an effort to eradicate TB, the government has launched a TB-DM collaboration program in every health facility. This study aimed to determine the relationship between readiness program officer and the implementation of the TB-DM collaboration program at Semarang Community Health Center, Central Java. Subjects and Method: This study was an observational study with a cross-sectional design conducted at community health center, Semarang. A sample of 37 TB service providers. The data were collected by questionnaire and in-depth interview. The data was analysed by Chi-square. Results: A total of 71.6% (53 respondents) of 74 respondents have implemented the TB-DM collaboration program well. As many as 87.7% (65 respondents) have satisfactory readiness in implementing the TB-DM collaboration program. Readiness and implementation of the TB-DM collaboration program were significantly related (p< 0.001). Conclution: The readiness of officers and the implementation of the TB-DM collaboration program are mostly good, and have a close relationship. Keywords: TB, DM, Collaboration, implementation Correspondence: Dea Amarilisa Adespin. Public Health Departement, Universitas Diponegoro. Jl. Prof. Soedarto No.1269, Tembalang, Semarang, Central Java 50275 DOI: https://doi.org/10.26911/the7thicph.04.21