Handling of Scabies in Domestic Cat at Q-one Petklinik Surabaya

Background: Scabies is a skin disease caused by Sarcoptes scabiei. Scabies is transmitted through direct contact with animals affected by scabies or contact with the source of scabies mite in area of cat lives. Purpose: This study aims to determine the management of scabies at Q-One PetKlinik Surabaya. Method: Handling domestic cats affected by S.scabiei began with an interview with the owner and examinated with the physical condition of cats, and observed the body that was infected with S.scabiei. Scrabbing on scabs was conducted in the cat’s body which was located in the facial area, and followed by microscopic examination to observe the S.scabiei mite. Results: During April 2021, there were 13 domestic cats affected by scabies. Treatment for scabies was by administrated drugs containing 5% Permethrin. Cats were also given supportive therapy by grooming with antiectoparasite shampoo after two weeks from being given drugs. Conclusion: Handling cases of scabies in domestic cats at Q-One PetKlinik Surabaya is performed by cleaning the scab, applying an ointment containing 5% permethrin, and giving anti-histamine and anti-parasitic as well as providing supportive therapy in the form of grooming using shampoo containing anti-ectoparasites.

A unique group of scabies mite pseudoproteases promotes cutaneous blood coagulation and delays plasmin-induced fibrinolysis

Background Scabies, a highly contagious skin disease affecting more than 200 million people worldwide at any time, is caused by the parasitic mite Sarcoptes scabiei. In the absence of molecular markers, diagnosis requires experience making surveillance and control challenging. Superficial microthrombi in the absence of vasculitis in scabies-affected skin are a recognised, yet unexplained histopathological differential of scabies infection. This study demonstrates that a family of Scabies Mite Inactivated Cysteine Protease Paralogues (SMIPP-Cs) excreted by the mites plays a role in formation of scabies-induced superficial microthrombi. Methodology/Principal findings A series of in vitro and ex vivo experiments involving two representative recombinant SMIPP-Cs was carried out. In the presence of SMIPP-Cs, the thrombin clotting time (TCT), fibrin formation and plasmin induced fibrinolysis were monitored in vitro. The ultrastructure of the SMIPP-C—modulated fibrin was analysed by Scanning Electron Microscopy (SEM). Immuno-histological analyses were performed ex vivo, to localise the SMIPP-C proteins within scabies infected skin biopsies. SMIPP-Cs displayed pro-coagulant properties. They bound calcium ions, reduced the thrombin clotting time, enhanced the fibrin formation rate and delayed plasmin-induced fibrinolysis. The SMIPP-Cs associated with fibrin clots during fibrinogen polymerisation and did not bind to preformed fibrin. Scanning electron microscopy revealed that the fibrin clots formed in the presence of SMIPP-Cs were aberrant and denser than normal fibrin clots. SMIPP-Cs were detected in microthrombi which are commonly seen in scabietic skin. Conclusions/Significance The SMIPP-Cs are the first scabies mite proteins found in sub-epidermal skin layers and their pro-coagulant properties promote superficial microthrombi formation in scabetic skin. Further research is needed to evaluate their potential as diagnostic or therapeutic target.

Molecular drug targets for scabies: a medicinal chemistry perspective

Sarcoptes scabiei is a causative organism for scabies that affects an estimated global population of 300 million and remains a disease of significant concern. Recently, a number of potential drug targets were identified for scabies, including hydrolytic enzymes, inactivated paralogues of hydrolytic enzymes, inhibitors of host proteolytic enzymes and other proteins of interest. These discoveries remain confined to academic laboratories and institutions, failing to attract interest from researchers in commercial drug development. Here, we summarize the latest developments in the scabies mite biology and the drug targets that were subsequently identified, and we propose several peptide and nonpeptide ligands targeting the hot spots for protein–protein interactions. We also identify gaps in the development of ligands as inhibitors or modulators of these macromolecules.

Acquisition Rate of Scabies in Employees After Care of an Undiagnosed Crusted Scabies Patient

Background: Scabies is a contagious dermatosis caused by human mites, (Sarcoptes scabei, variant hominis). In crusted (Norwegian) scabies, the burden of mite infestation is higher and up to 2 million per person, facilitating easy skin-to-skin transmission and nosocomial transmission. We describe a case of undiagnosed crusted scabies and subsequent transmission to employees in our hospital. Methods: A 90-year-old female was admitted to our 636-bed, nonprofit, academic hospital for 22 days prior to diagnosis of crusted scabies by skin scraping. The patient was admitted to 2 different medical-surgical wards and the medical intensive care unit. We collected healthcare worker (HCW) demographics, including department of service, age, sex, pregnancy, and breastfeeding status in those who were at risk of exposure. We interviewed HCWs at 2 time points and collected information related to infestation, allergies to treatment, acceptance of empiric treatment, and whether employee was furloughed. Results: On initial screening, 20 of 124 at-risk HCWs had symptoms (Fig.). Most had a “new onset raised red rash or new pimple like rash (not on face), or linear rash” and 4 had “new onset uncontrollable itching.” All 124 HCWs were contacted 28 days later. One HCW that had not been compliant with prophylaxis became symptomatic and was diagnosed with scabies by dermatology. Of the remaining 20 HCWs, 3 were still having symptoms (2 had itching and 1 had a rash and a scrape performed by dermatology with confirmation of mites). All 3 were retreated with ivermectin. Overall, 21 of 124 exposed HCWs were ultimately symptomatic. Conclusions: During a 22-day admission of an undiagnosed and unisolated elderly patient with crusted scabies, the scabies mite was transmitted to 21 HCWs for an acquisition rate of 17%. Persistence of symptoms after treatment with permethrin occurred in 14%. The infectivity of this disease necessitates early recognition and infection control measures.Funding: NoneDisclosures: None

Utilization of Human Skin Equivalent in Research of Scabies Pathogenesis

Scabies is a tropical disease that requires large-scale control. Research on the pathogenesis of scabies in Indonesia is still rare. Meanwhile, in tropical countries, studies related to the course of scabies have been done quite a lot, one of which is by using a human skin model. The purpose of writing this article is to discuss the use of human skin modeling technology in researching the course of scabies. Research using HSE demonstrated modulation of cytokine secretion, expression of cell adhesion molecules, and gene expression in response to the scabies mite extract molecule.

The true identity of Pascal’s mite and the diachronic use of ciron

The identity of Blaise Pascal’s mite is examined. Linguistics, morphology, habitat and size reveal that Pascal’s mite is not Acarus siro L., as usually thought, but Sarcoptes scabiei (L.). The diachronic use of the popular term ciron is explored from a number of different perspectives. In everyday language and in Pascal’s time, the term used to designate A. siro L. was mite (used singly, in English and French) and not ciron, a word restricted to the scabies mite; its use is also investigated. The classification of mites in the 17th and 18th centuries is reviewed.

Scabies

Infestation of the human by the parasitic scabies mite, Sarcoptes scabiei var hominis, is discussed in this chapter on scabies. The life cycle of the mite is outlined, and the epidemiology and modes of transmission are discussed. The clinical presentations of classical scabies, crusted scabies, scabies in someone with HIV, and scabies incognito are all explained in turn. Diagnosis is discussed and first-line management with permethrin, with malathion as a second-line alternative, are explained. The accompanying practical advice on how to use the treatments that should be given to patients is detailed. The treatment of crusted scabies, considerations in pregnancy/breastfeeding, and emerging resistance are mentioned.