The biosynthetic-secretory pathway, supplemented by recycling routes, specifies epithelial membrane polarity

In prevailing epithelial polarity models, membrane-based polarity cues (e.g., the partitioning-defective PARs) position apicobasal cellular membrane domains. Intracellular vesicular trafficking expands these domains by sorting apicobasal cargo towards them. How the polarity cues are polarized and how sorting confers long-range vesicle directionality is still unclear. Here, a systems-based approach using two-tiered C. elegans genomics-genetics screens identifies trafficking molecules that are not implicated in apical sorting yet polarize apical membrane and PAR complex components. Live tracking of polarized membrane biogenesis suggests that the biosynthetic-secretory pathway, linked to recycling routes, is asymmetrically oriented towards the apical domain during its biosynthesis, upstream of PARs and independent of polarized target domains. This mode of membrane polarization could offer solutions to questions of current models of polarity and polarized trafficking.

The Forward Modeling for Surface-borehole Observation Responses of Polarized Target

Surface-hole induced polarization method is a typical deep resource exploration technology, which plays an important role in the mineral survey. The traditional surface-hole induced polarization method is mainly to observe a single polarized secondary field. At present, the time spectrum observation of polarization fields is becoming more and more popular, which greatly enriches the interpretation technology of induced polarization data. In this work, the time spectrum induced polarization method is expounded, the decay polarization fields were numerical calculated and analyzed for the typical 2-dimensional geoelectric model. The results show that for a single polarized target, the time spectrum obtained from different azimuth observation responses are basically the same, which can effectively reflect the time-varying characteristics of the polarized fields. The observation responses of polarized target at different depths can still reflect the time spectrum of decay fields. The main change of the observed response of the polarized target closer to the borehole is the response amplitude. The conclusions and simulations of this study can provide working mode for relevant research and reference for similar work.