7P pediatrics — Medicine of Development and Health Programming

The transition to personalized, predictive, preventive and participatory medicine, due, among other things, to the achievements of fundamental science, digitalization and the development of information and communication technologies, naturally demanded changes in childrens health care. New organizational, methodological and technological changes that have taken place to ensure a highly dynamic, adapted, and, at the same time, permanent provision of the medical process anywhere and at any time, have led to the need for specialists of a new formation a pluriexpert team ready to work in new conditions: how to participate in research in fundamental science, and to the introduction of innovative methods in clinical practice, medical and social support and the educational process. In these conditions, the strategic goal set for pediatrics to analyze absolutely all aspects of health: somatic, neuropsychic, emotional and psycho-social, in the process of growth and development of a child from conception/birth to adolescence / adulthood was implemented in a new direction of clinical and fundamental medicine developmental pediatrics and child health programming. 7P-pediatrics: Programming the development and health of the child, Preventive, Predictive, Personalized, Participatory, Polyprofessional (Pluriexpert), Progressive medicine for children, in which the results of scientific work based on fundamental data and ideas of neurosciences about progressive development and modern methodology of educational support of the entire medical process are fully translated into a clinical practice.

Application of Simulation Training in the Framework of the Course "Community Rescuer"

Simulation training is an integral part not only in the training of doctors and medical students, but also among everyone who does not have a medical education. The created course "Public Rescuer" allows you to work out the theoretical skills using modern technologies on the basis of the Center for Simulation Medicine of the Institute of Fundamental Medicine and Biology of Kazan Federal University.

Problems of Pathogenesis and Pathogenetic Therapy of COVID-19 from the Perspective of the General Theory of Pathological Systems (General Pathological Processes)

The COVID-19 pandemic examines not only the state of actual health care but also the state of fundamental medicine in various countries. Pro-inflammatory processes extend far beyond the classical concepts of inflammation. They manifest themselves in a variety of ways, beginning with extreme physiology, then allostasis at low-grade inflammation, and finally the shockogenic phenomenon of “inflammatory systemic microcirculation”. The pathogenetic core of critical situations, including COVID-19, is this phenomenon. Microcirculatory abnormalities, on the other hand, lie at the heart of a specific type of general pathological process known as systemic inflammation (SI). Systemic inflammatory response, cytokine release, cytokine storm, and thrombo-inflammatory syndrome are all terms that refer to different aspects of SI. As a result, the metabolic syndrome model does not adequately reflect the pathophysiology of persistent low-grade systemic inflammation (ChSLGI). Diseases associated with ChSLGI, on the other hand, are risk factors for a severe COVID-19 course. The review examines the role of hypoxia, metabolic dysfunction, scavenger receptors, and pattern-recognition receptors, as well as the processes of the hemophagocytic syndrome, in the systemic alteration and development of SI in COVID-19.

Development of the Method to Produce Functionally Active Recombinant Streptavidin in Escherichia coli Cells

Streptavidin is a homotetrameric protein produced by Streptomyces avidinii, each subunit of which binds biotin (vitamin H), forming a stable complex (Kd = 10-15 М). Streptavidin-biotin coreaction is widely used in analytical systems, for targeted delivery of compounds, for affinity purification, etc. The aim of this study was to develop a rational technique to produce functionally active recombinant streptavidin. Recombinant Escherichia coli strains producing minimal core and full-sized streptavidin variants were obtained. The E. coli BL21 Codon Plus (DE3) RIPL, as host cells, and the pET19b plasmid carrying gene of minimally-sized core (miniSAV) or full-sized (SAV) streptavidin were used. Synthesis of miniSAV results in its localization as insoluble inclusion bodies. Denatured miniSAV yield was 130 mg per liter of E. coli c ulture. T he r enaturation g ives o nly 10- 15 % of the functionally active protein. Full-sized streptavidin localizes in the cytoplasm in a soluble state, but its toxicity causes low yield of the protein (10-13 mg per liter of the culture). The induction of SAV synthesis at the end of the logarithmic stage of cell growth was found to increase the yield of SAV approximately 2-fold. The yield of functionally active protein was 30 mg per liter culture. SAV was produced practically in individual state after affine chromatography on 2-iminobiotin agarose. One molecule of full-sized streptavidin bound 3.9 biotin molecules as was shown by colorimetric analysis using HABA (4-hydroxyazobenzene-2-carboxylic acid). Both streptavidins form sandwichtype complexes with biotinylated molecules in solid-phase microassay conditions. E. coli BL21 Codon Plus (DE3) RIPL/pET19bSAV strain was stable during storage with 20 % glycerol at -70 °С, which was shown by repeated two-year reseeding. The streptavidin producing strain (E. coli BL21 Codon Plus (DE3) RIPL/pET19bSAV) is deposited in the Collection for extremophile microorganisms and type cultures (Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk), No. 3505. The method for producing functionally active recombinant streptavidin developed in this study ensures its availability for biotechnological research

Optimization of the Extraction Procedure of Apixaban from Dried Rat Plasma Spots

Aims: Apixaban is an anticoagulant used to treat and prevent blood clots, as well as to prevent stroke in people with atrial fibrillation. The dried spot analyses, including dried blood spots and dried plasma spots, are used to simplify techniques for determining drug concentrations in blood and plasma. In this case, equipment with highly sensitive detector is required, for example, mass spectrometer, as well as a high level of drug extraction from the dried spot. In this work, apixaban extraction from dried plasma spots (DPS) was studied in order to determine the optimal parameters of the extraction method. Study Design: Short Research Articles. Place and Duration of Study: Core Facility of Mass Spectrometric Analysis, Institute of Chemical Biology and Fundamental Medicine SB RAS, between September 2019 and February 2020. Methodology: The organic extraction method was chosen for evaluation as the most suitable for LC-MS assay. Several parameters: percentage of organic solvent, presence or absence of 0.1% formic acid (FA), time, volume and temperature of extraction were investigated to find the best combination for recovery of apixaban from DPS for further LC-MS analysis. Results: The results showed that the main influence on the extraction is the composition of the solvent, volume of solvent, as well as temperature and time of extraction. Pure acetonitrile is the worst solvent for extracting apixaban from DPS. Solvents: MeOH:H2O (100:0, v: v), MeOH: 0.1% FA in H2O (80:20, v:v), ACN: 0.1% FA in H2O (90:10, v:v) or ACN:MeOH (90:10, v:v) provide better recovery of apixaban. The optimum extraction parameters were as follows: 90% acetonitrile concentration, extraction temperature of 40°C, extraction time of 15 min, and solvent volume of 100

Selected Papers from 2019 IEEE Eurasia Conference on Biomedical Engineering, Healthcare and Sustainability (IEEE ECBIOS 2019)

Recently, healthcare has undergone a sector-wide transformation thanks to advances in computing, networking technologies, big data, and artificial intelligence. Healthcare is not only changing from being reactive and hospital-centered to preventive and personalized, but it is also changing from being disease focused to well-being centered. Healthcare systems, as well as fundamental medicine research, are becoming smarter and enabled in biomedical engineering. This special issue on “Selected Papers from 2019 IEEE Eurasia Conference on Biomedical Engineering, Healthcare, and Sustainability (IEEE ECBIOS 2019)” selected nine excellent papers from 160 papers presented at IEEE ECBIOS 2019 on the topics of environmental health sciences and public health. Our aim is to encourage scientists to publish their experimental and theoretical research to promote scientific predictions and impact assessments of global change and development.

Selected Papers From 2019 IEEE Eurasia Conference on Biomedical Engineering, Healthcare and Sustainability (IEEE ECBIOS 2019)

The 2019 IEEE Eurasia Conference on Biomedical Engineering, Healthcare, and Sustainability (IEEE ECBIOS 2019) was held in Okinawa, Japan on 31 May–3 June 2019, and provided a unified communication platform for researchers in the fields of biomedical engineering, healthcare, and sustainability. Recently, healthcare has undergone a sector-wide transformation thanks to advances in computing, networking technologies, big data, and artificial intelligence. Healthcare is not only changing from being reactive and hospital-centered to preventive and personalized, but it is also changing from being disease focused to well-being centered. Healthcare systems, as well as fundamental medicine research, are becoming smarter and enabled in biomedical engineering. Furthermore, with cutting edge sensors and computer technologies, healthcare delivery could also yield better efficiency, higher quality, and lower cost. This Special Issue on “Selected Papers from the 2019 IEEE Eurasia Conference on Biomedical Engineering, Healthcare, and Sustainability (IEEE ECBIOS 2019)” is expected to select excellent papers that were presented in IEEE ECBIOS 2019 on the topic of sustainability. It will link several disciplines, including the environmental, cultural, economic, and social sustainability of human beings, which provide an advanced forum for studies related to sustainability and sustainable development. Our aim is to encourage scientists to publish their experimental and theoretical research relating to natural sciences, social sciences, and humanities in as much detail as possible in order to promote scientific predictions and impact assessments of global change and development.

Neurobiological risk factors in the formation of internet addiction in adolescence: vital hypotheses and the nearest perspectives

Objectives. The review summarizes the currently available data on neurobiological risk factors for the formation of Internet addiction (IA) in adolescents, and also indicates the most promising areas of research in this area. Background. The rapid emergence and spread of IA in teenage populations, combined with the rapid change in consumed content due to the general availability of mobile access to the network and the emergence of new devices, poses new challenges for classical psychology and fundamental medicine that need to be addressed urgently. Like most other chronic psychopathological conditions, pathological heart failure belongs to the group of multifactorial polygenic diseases, where in each case there is a unique combined effect of congenital features of the functioning of higher nervous activity, some of which are genetically determined (structural features of the structure of the nervous tissue, spectrum of secretion, degradation and the reception of neurotransmitters) with the influence of many environmental factors (family, social, ethnocultural). Methodology. To date, a large number of studies have been conducted to study the pathogenesis of IA using various neuroimaging techniques. Using twin studies while taking into account ethnic differences, the genetic component of susceptibility to IA was established, in addition, a serious comorbidity of Internet addiction was revealed. Conclusions. One of the most important fundamental tasks of studying Internet addiction is the identification of specific genes and neurotransmitter associations that are respon¬sible for the predisposition to the formation of Internet addiction, which will allow searching for new therapeutic targets and ways for early prevention with an assessment of the degree of genetic risk.