Evaluation of recovery of oxygen saturation and rate of perceived exertion in different body positions after submaximal exercise test in normal healthy individual

Background: Oxygen saturation is the fraction of oxygen saturated hemoglobin relative to total hemoglobin in blood. Normal oxygen saturation level in humans are 95-100 percent. Breathlessness is an unpleasant sensation of rapid or difficult breathing. The reason behind the subject experiencing breathlessness is that the body demands more oxygen than it supplies. Thus this study is conducted to Evaluate Recovery of Oxygen saturation and Rate of perceived exertion in different body positions after submaximal exercise testing in normal healthy individuals. Methodology: It was Pre and Post Study Design with 6 months duration including 40 healthy students recruited by purposive sampling method. The outcome measure was Recovery of Oxygen saturation and rate of perceived exertion. Result – Using the Paired t test, this shows that fastest recovery of oxygen saturation occurs in prone position that is within 1 min (99±0.96) than supine within 3 min (99±0) than upright sitting within 3 min (99±0) and fastest recovery of rate of perceived exertion occurs in prone position that is within 1 min (0.825±0.99) than supine within 3 min (0±0) than upright sitting within 3 min (0±0). Conclusion - From the present study we evaluate the Recovery of oxygen saturation and RPE in supine, prone, upright sitting position and we found that the fastest recovery of oxygen saturation and Rate of perceived exertion occurs in prone position than supine and upright sitting position after submaximal exercise testing in normal healthy individual.

A COMPARATIVE STUDY RELATING WATER DRINKING TEST AND INTRA OCULAR PRESSURE IN DIFFERENT AGE GROUPS

AIM:To determine the behavior of IOP in different age groups among normal populations after water drinking test for a period of time. MATERIALS AND METHODS:This is a prospective study involving normal healthy individual taken from different age groups 20-50 years,with no relevant past medical and surgical history. Patients were instructed to drink 1Litre of water over a period of 5-10minutes after 8 hours of overnight fasting and the IOP was checked before drinking water and every 15minutes for 1 hour period followed by 2 hours after drinking water using schiotz tonometer.The difference in baseline IOP and IOP at 1 hour and 2 hour was evaluated among different age groups . RESULT: 100 patients from the age groups 20-50 years were studied .The mean baseline IOP was in the range of 12.4mmHg and the mean IOP after 1 hour was 15.3mmHg.A difference of 4-4.5 mmHg were noted in the age group of 40- 50 years .The IOP was gradually decreasing to 12.78mmHg over a period of 2 hours after WDT. CONCLUSION: The higher age group the more the rise in the IOP after WDT which concludes that as the age increases drinking litres of water over a few minutes will substantially increase the IOP,which might damage the optic nerve.

Pollen Allergy; Mechanism and Etiology

The term allergy refers to an exaggerated immune response to a foreign molecule. In a normal healthy individual, this foreign molecule will act as a harmless antigen, as it will be recognized & cleared by the immune system without causing any significant damage to host tissues. In certain individuals, such antigens stimulate immune system in such a way that a series of exaggerated immune response are generated against it, leading to significant tissue injury and damage to the host. Such as exaggerated immune response may also cause death of the patient. Such antigen is termed as an allergen. Allergy is categorised under Immunoglobulin E (IgE) mediated type I hypersensitivity reactions. These reactions occur when an antigen acting as an allergen attacks the immune system of the host and causes excessive stimulation of mast cells and basophils. This is followed by the release of allergy mediators which are responsible for causing local or systemic anaphylaxis, allergic asthma, allergic rhinitis, conjuctivitis. Type I allergens are of various types, the mechanism of action is same; however underlying factors may differ in each type. This article is about pollen allergens, in specific & will discuss the common sources of pollen allergens in Pakistan, the relevance and the factors behind pollen allergy.

Serum Ferritin Status of Patients with Type 2 DM Attending OPD of BIRDEM General Hospital

Background: In recent times much is talked about of serum ferritin, an acute phase reactant a marker of iron stores in the body and its association with diabetes mellitus. Studies implicate that increased body iron stores and subclinical hemochromatosis has been associated with the development of glucose intolerance, type 2 diabetes and its micro as well as macrovascular complications. Material & Methods: This study was carried out to examine and to observe for any relationship between serum ferritin with Type 2 diabetes mellitus. Our study populations were included 163. Among them 81 type 2 diabetes patients as a case (M=49,F=32, mean 44.68 age in years)and 82 normal healthy individual as a control ( M=35, F=47 , mean 34.71 in years). Results: Majority were healthy outpatients who had come for regular checkup and were matched with controls. Serum ferritin and FBS were estimated and other investigations. Results showed that although Serum ferritin was in the normal range value it was increased in type 2 diabetes patients than in controls and was statistically significant, we did get a positive correlation with duration of diabetes. It can be concluded that there were positive associations between serum ferritin and FBG, age, sex among study groups. Conclusion: In conclusion our study shows that there is significant correlation between increased serum ferritin in diabetes compared to individuals with normal blood sugars in this part and hyper ferritinemia may be one of the causes for development of insulin resistance before overt diabetes. Anwer Khan Modern Medical College Journal Vol. 11, No. 1: Jan 2020, P 7-10

Atrial fibrillation: a rare but probable adverse effect of sildenafil

Knowledge of the cardiovascular effects of sildenafil is important for a number of reasons. The main risk factors for the erectile dysfunction (hypercholesterolemia, hypertension, diabetes, smoking) are the same as those for coronary artery disease. The reported cardiovascular side effects of sildenafil in normal healthy population are minor and mainly associated with vasodialation (i.e. headache, flushing and small decrease in systolic and diastolic blood pressure). Authors report the occurrence of atrial fibrillation after the use of sildenafil in a normal healthy individual. To the best of our knowledge, there are only few case reports in the literature showing occurrence of atrial fibrillation with use of sildenafil.

Can serum thioredoxin levels predict sensitivity to platinum in lung cancer?

e22062 Background: Our laboratory has found that cisplatin resistant lung cancer cells possess high levels of ROS (reactive oxygen species). Low intracellular TRX1 (thioredoxin) levels due to increased secretion of the thioredoxin-1 (TRX1) is the primary reason for high intracellular ROS levels. We have further shown that reconstituting TRX1 protein in cisplatin-resistant cells can restore sensitivity to cisplatin. Conversely, silencing TRX1 in parental cells reduced the sensitivity to cisplatin (MCT11:604, 2012). Thus, it is possible that serum TRX1 levels may be a useful marker to predict sensitivity to platinum containing regimens. Methods: Serum samples were obtained before, during, and after platinum chemotherapy. TRX1 was determined by sandwich ELISA with two TRX1 antibodies. Samples, standards, and reagent blank were incubated with precoated plate (IBL). Labeled antibody solution was added followed by Chromagen substrate and subjected to plate reader. The value on normal healthy individual is less than 5 ng/ml. The lowest limit of detection is 1 ng/ml. Results: 11 patients (pts) were entered into the study. 6 pts had small cell lung cancer (SCLC) and 5 had non small cell lung cancer (NSCLC). 2 SCLC pts died before treatment and couldn’t be assessed and 2 pts are too early to assess response. In these lung cancer pts, the baseline levels ranged from 0 to 96.5 ng/ml. Thus far, 4 pts (1 SCLC and 3 NSCLC) who had baseline TRX1 levels of 0, 0, 0 and 3 responded to chemotherapy. An additional SCLC pt with very high levels of TRX1 (96) also responded to chemotherapy. However, this pt also had a recent operation for NSCLC and the baseline blood sample was obtained after the operation, which may not be the appropriate baseline. 2 pts with baseline TRX1 levels of 31.1 and 6.5 did not respond to treatment. TRX1 levels increased during chemotherapy, especially when given concurrently with radiation. However, in pts who responded, TRX1 levels decreased at the end of treatment. Conclusions: The study is currently ongoing. Our initial results suggest that low TRX1 levels may indicate possible future response to platinum containing regimens. While TRX1 levels may increase during treatment, in pts who respond to treatment, TRX1 levels often decrease back to baseline.

Single nucleotide polymorphisms (SNPs) in human lactoferrin geneThis paper is one of a selection of papers published in this Special Issue, entitled 7th International Conference on Lactoferrin: Structure, Function and Applications, and has undergone the Journal's usual peer review process.

The lactoferrin protein possesses antimicrobial and antiviral activities. It is also involved in the modulation of the immune response. In a normal healthy individual, lactoferrin plays a role in the front-line host defense against infection and in immune and inflammatory responses. Whether genomic variations, such as single nucleotide polymorphisms (SNPs), have an effect on the structure and function of lactoferrin protein and whether these variations contribute to the different susceptibility of individuals in response to environmental insults are interesting health-related issues. In this study, the lactoferrin gene was resequenced as part of the Environmental Genome Project of the National Institute of Environmental Health Sciences, which operates within the National Institutes of Health. Ninety-one healthy donors of different ethnicities were used to establish common SNPs in the exons of the lactoferrin gene in the general population. The data will serve as a basis from which study the association of lactoferrin polymorphism and disease.