HighlightsA detection clamp for the rind-pith adhesive strength of corn stalks based on peeling method was designed.A rind-pith adhesive strength detection method was proposed taking average peeling strength as the test index.The characteristics of rind-pith adhesive strength were analyzed, and a mathematical model between average peeling strength and influencing factors was established.Abstract. Rind-pith separation of corn stalks is one of the effective measures for realizing high-value utilization of stalks. Therefore, it is necessary to study rind-pith adhesive strength of stalks and its influencing factors to determine effective separation methods. In this study, a peeling method was put forward and single factor and multi-factor tests were carried out to explore the rind-pith adhesive strength. The analysis of single factor tests showed that sampling location, moisture content, and peeling speed were all significant on the average rind-pith peeling strength and the Fp = 25.70 > F0.05 (4, 20) = 2.87, Fm = 112.46 > F0.05 (4, 20) = 2.87 and Fs = 231.21 > F0.05 (6, 28) = 2.45. The mathematical model between average peeling strength and influencing factors was established through multi-factor tests. The model was analyzed and optimized, and the significance sequence of influencing factors is sampling location > moisture content > peeling speed, and the effect of interaction between moisture content and sampling location was significant (P<0.05). It can be concluded that the rind-pith peeling strengths of internode sections among 1st-7th internodes of corn stalks were 360.09-450.01 N/m, which are smaller and more favorable for mechanical separation for stalks with moisture content of 15%-20% and at peeling speed of 30-40 mm/min. The results of this study may provide a theoretical reference for the modeling of corn stalks and the subsequent simulation and optimization of rind-pith separation machines. Keywords: Average peeling strength, Adhesive strength, Corn stalks, Rind-pith Separation.