Early-Life Adversity Leaves Its Imprint on the Oral Microbiome for More Than 20 Years and Is Associated with Long-Term Immune Changes

The early-life microbiome (ELM) interacts with the psychosocial environment, in particular during early-life adversity (ELA), defining life-long health trajectories. The ELM also plays a significant role in the maturation of the immune system. We hypothesised that, in this context, the resilience of the oral microbiomes, despite being composed of diverse and distinct communities, allows them to retain an imprint of the early environment. Using 16S amplicon sequencing on the EpiPath cohort, we demonstrate that ELA leaves an imprint on both the salivary and buccal oral microbiome 24 years after exposure to adversity. Furthermore, the changes in both communities were associated with increased activation, maturation, and senescence of both innate and adaptive immune cells, although the interaction was partly dependent on prior herpesviridae exposure and current smoking. Our data suggest the presence of multiple links between ELA, Immunosenescence, and cytotoxicity that occur through long-term changes in the microbiome.

Unsafe at Any Time

The chapter “Unsafe at Any Time” examines Generation Disaster’s perceptions of safety, risk, fairness, and other consequences of their early environment, and it explores whether these concerns relate to the high rates of anxiety and depression reported by this generation relative to other cohorts. It also describes the methodological challenges that are inherent in trying to make valid comparisons to previous ages, given all of the dramatic societal changes of the past few decades, and the chapter discusses the impact of the belittling descriptions of this cohort by elders as being overly sensitive or generally inferior to previous generations, when in reality their often cautious worldview may be an adaptive response to a genuinely more dangerous world.

The genetics of eating behaviors: research in the age of COVID-19

How much pleasure we take in eating is more than just how much we enjoy the taste of food. Food involvement - the amount of time we spend on food beyond the immediate act of eating and tasting - is key to the human food experience. We took a biological approach to test whether food-related behaviors, together capturing food involvement, have genetic components and are partly due to inherited variation. We collected data via an internet survey from a genetically informative sample of 419 adult twins (114 monozygotic twin pairs, 31 dizygotic twin pairs, and 129 singletons). Because we conducted this research during the pandemic, we also ascertained how many participants had experienced COVID-19-associated loss of taste and smell. Since these respondents had previously participated in research in person, we measured their level of engagement to evaluate the quality of their online responses. Additive genetics explained 16-44% of the variation in some measures of food involvement, most prominently various aspects of cooking, suggesting some features of the human food experience may be inborn. Other features reflected shared (early) environment, captured by respondents' twin status. About 6% of participants had a history of COVID-19 infection, many with transitory taste and smell loss, but all but one had recovered before the survey. Overall, these results suggest that people may have inborn as well as learned variations in their involvement with food. We also learned to adapt to research during a pandemic by considering COVID-19 status and measuring engagement in online studies of human eating behavior.

Rhabdophane Th-Pb ages indicate reactivation of Mesoarchean structures in west Pilbara Craton during breakup of Greater India and Australia-Antarctica

Uranium-Th-Pb dating of phosphate minerals in very low-grade metasedimentary rocks from the Archean Pilbara Craton, Western Australia, has revealed a long history of deformation and fluid flow during the Paleoproterozoic. However, this technique has not detected evidence for fluid flow along craton margins during Phanerozoic rifting and breakup. We report the use of in situ Th-Pb geochronology of rhabdophane, a hydrous light rare earth element phosphate, to date fluid flow in shale from the 2.76 Ga Mount Roe Basalt from drill hole number 6 of the Archean Biosphere Drilling Program (ABDP6), northwestern Pilbara Craton. Thorium-Pb dating of rhabdophane in carbonaceous shale yields three main populations with weighted mean 208Pb/232Th ages of 152 ± 6 Ma, 132 ± 4 Ma, and 119 ± 4 Ma, which indicates phosphate growth up to 2.64 b.y. after deposition. The rhabdophane ages are coeval with three major breakup events in eastern Gondwana: separation of Southwest Borneo and Argoland from Australia (ca. 156–152 Ma), breakup of Greater India from Australia (ca. 140–135 Ma), and separation of Greater India/India from Antarctica (ca. 123 Ma). The proximity of drill hole ABDP6 to major Mesoarchean faults and shear zones on the craton margin, which are parallel to rift propagation and basin development, points to episodic reactivation of ancient crustal structures >2.8 b.y. after their formation. Our results also highlight the potential of rhabdophane as a U-Th-Pb geochronometer for dating low-temperature (<200 °C) fluid flow and hydrous alteration. The migration of Mesozoic fluids through Archean shales adds weight to questions about the origin of geochemical signals in ancient altered rocks and how to extract information about the early environment and biosphere.

Start on the Right Foot: Choice in a Complex Early Environment Boosts Coping Abilities in the Domestic Fowl Chick

Early experience of a complex environment can improve biologically relevant traits related to coping abilities. However, the mechanisms underlying these positive effects are not well explored. We hypothesised that the possibility to express a choice, leading to increased control over the environment, could play an important role. In a balanced design, laying hen hatchlings were reared either in a ‘No-Choice’ environment (single litter and perch type) or a ‘Choice’ environment (four different litter and perch types). Immunological and behavioural indicators of birds’ coping abilities were explored at three weeks of age. Chicks from Choice environments had higher natural antibody titres, lower H/L ratios, required more attempts for tonic immobility induction and were more successful gaining novel food rewards in a repeated challenge test. Results imply that their improved natural immune response better prepared Choice chicks for coping with pathogenic challenges, that they experienced less chronic stress and were less fearful. To conclude, the increased potential for control and stimulation arising from making decisions in an early complex environment seemed to better prepare birds for a variety of challenges to come, boosting their adaptive capacities and their ability to make the most of opportunities.

Regional Differences in Brain Plasticity and Behaviour as a Function of Sex and Enrichment Type: Oxytocin Matters

The early environment is critical to brain development, but the relative contribution of physical vs. social stimulation is unclear. Here, we investigated in male and female rats the response to early physical and social environmental enrichment in relation to oxytocin (OT) and brain-derived neurotrophic factor (BDNF) expression. The findings show that males and females respond differently to prolonged sensorimotor stimulation from postnatal day 21-110 in terms of functional, structural and molecular changes in the hippocampus vs. medial prefrontal cortex (mPFC). Physical enrichment promoted motor and cognitive functions and hippocampal BDNF mRNA and protein expression in both sexes. Combined physical and social enrichment, however, promoted functional and structural gain predominantly in females. These changes were accompanied by elevated plasma oxytocin (OT) levels and BDNF mRNA expression in the mPFC while the hippocampus was not affected. Administration of an OT antagonist in females blocked the beneficial effects of enrichment and led to reduced cortical BDNF signaling. These findings suggest that an OT-based mechanism selectively stimulates a region-specific BDNF response which is dependent on the type of experience.

Interaction between Oxytocin Genotypes and Early Experience Predicts Quality of Mothering and Postpartum Mood

Individual differences in maternal behavior are affected by both early life experiences and oxytocin, but little is known about genetic variation in oxytocin genes and its effects on mothering. We examined two polymorphisms in the oxytocin peptide gene OXT (rs2740210 and rs4813627) and one polymorphism in the oxytocin receptor gene OXTR (rs237885) in 187 Caucasian mothers at six months postpartum. For OXT, both rs2740210 and rs4813627 significantly associated with maternal vocalizing to the infant. These polymorphisms also interacted with the quality of care mothers experienced in early life, to predict variation in maternal instrumental care and postpartum depression. However, postpartum depression did not mediate the gene-environment effects of the OXT SNPs on instrumental care. In contrast, the OXTR SNP rs237885 did not associate with maternal behavior, but it did associate with pre-natal (but not post-natal) depression score. The findings illustrate the importance of variation in oxytocin genes, both alone and in interaction with early environment, as predictors of individual differences in human mothering. Furthermore, depression does not appear to have a causal role on the variation we report in instrumental care. This suggests that variation in instrumental care varies in association with a gene-early environment effect regardless of current depressive symptomatology. Finally, our findings highlight the importance of examining multiple dimensions of human maternal behavior in studies of genetic associations.

Interaction between Oxytocin Genotypes and Early Experience Predicts Quality of Mothering and Postpartum Mood

Individual differences in maternal behavior are affected by both early life experiences and oxytocin, but little is known about genetic variation in oxytocin genes and its effects on mothering. We examined two polymorphisms in the oxytocin peptide gene OXT (rs2740210 and rs4813627) and one polymorphism in the oxytocin receptor gene OXTR (rs237885) in 187 Caucasian mothers at six months postpartum. For OXT, both rs2740210 and rs4813627 significantly associated with maternal vocalizing to the infant. These polymorphisms also interacted with the quality of care mothers experienced in early life, to predict variation in maternal instrumental care and postpartum depression. However, postpartum depression did not mediate the gene-environment effects of the OXT SNPs on instrumental care. In contrast, the OXTR SNP rs237885 did not associate with maternal behavior, but it did associate with pre-natal (but not post-natal) depression score. The findings illustrate the importance of variation in oxytocin genes, both alone and in interaction with early environment, as predictors of individual differences in human mothering. Furthermore, depression does not appear to have a causal role on the variation we report in instrumental care. This suggests that variation in instrumental care varies in association with a gene-early environment effect regardless of current depressive symptomatology. Finally, our findings highlight the importance of examining multiple dimensions of human maternal behavior in studies of genetic associations.

'Effective e-therapy engagement, and improving Maori families early environment’ In Conversation Prof Sally Merry

We are delighted to have the opportunity to talk to Professor Sally Merry, the Cure Kids Duke Family Chair in Child and Adolescent Mental Health.