Genetic, Reproductive and Hematological Toxicity Induced in Mice Exposed to Leachates from Petrol, Diesel and Kerosene Dispensing Sites

Background. With a population of over 165,000,000, growing at an average rate of 2.7% per annum and an economic growth rate of about 5.7% in the past five years, the market for refined petroleum products in Nigeria is growing. As a result, the number of filling stations is increasing. Objectives. The present study evaluated the reproductive and genetic toxicity of simulated leachate of soil from petrol, diesel and kerosene dispensing sites in a filling station using the murine sperm abnormality test, sperm count and bone marrow micronucleus assay. Methods. Simulated leachate of soil collected from petrol, diesel and kerosene dispensing sites in a filling station was intraperitoneally administered to mice at different concentrations. Bone marrow micronucleus assay was carried out after 5-days exposure, while sperm morphology assay was carried out 35 days from the first day of exposure. Alterations to hematological parameters were evaluated and physico-chemical analysis of the leachate samples was also carried out. Results. The results showed a significant (p<0.05) concentration-dependent increase in abnormal sperm cells and decrease in mean sperm count in all the samples tested. Increased induction of micronucleated polychromatic erythrocytes was observed in the exposed mice. Hematological analysis showed a significant (p<0.05) increase in the values of white blood cell count (WBC), lymphocytes, neutrophils, monocytes, eosinophils and mean corpuscular volume (MCV), while a significant (p<0.05) reduction in basophils, hemoglobin, mean corpuscular hemoglobin (MCH), packed cell volume and mean corpuscular hemoglobin concentration (MCHC) values were observed. Discussion. In the present study, simulated leachates from soil obtained from petrol, diesel and kerosene dispensing sites were shown to cause genomic disruptions in germ and somatic cells, and hematotoxicity in an animal model. These observed reproductive, genetic and hemato-toxicities are believed to be caused by the presence of lead, copper, mercury, polycyclic aromatic hydrocarbons, and benzene in the samples. Conclusions. This study showed the negative impact of petroleum products in the contamination of soil, with a capability of inducing genetic damage in somatic and germ cells of exposed plants and animals. Competing Interests. The authors declare no financial competing interests. Ethics Approval. The study was approved by the ethical committee of the Federal University of Technology, Akure, Ondo State, Nigeria.

Genotoxicity and antigenotoxicity study of aqueous and hydro-methanol extracts of Spondias mombin L., Nymphaea lotus L. and Luffa cylindrical L. using animal bioassays

Spondias mombin (Linn), Nymphaea lotus (Linn) and Luffa cylindrica (Linn) (syn Luffa aegyptiaca Mill) are plants traditionally used as food ingredients and in the management of diseases, including cancer, in Nigeria. Despite the therapeutic potentials attributed to these plants, reports on their genotoxicity are scanty. In this study, the genotoxicity of the aqueous and hydro-methanol extract of these plants was evaluated using mouse bone marrow micronucleus and sperm morphology assays. Antigenotoxicity was assessed by the bone marrow micronucleus test. The highest attainable dose of 5 000 mg/kg according to OECD guidelines was first used to assess acute toxicity of the aqueous and hydro-methanol extracts in Swiss albino mice. For each extract, there were five groups of mice (n=4/group) treated with different concentrations of the extract as against the negative and positive control group for the genotoxicity study. In the antigenotoxicity study, five groups of mice were exposed to five different concentrations of the extracts along with 60 mg/kg of methyl methane sulfonate (MMS), which was used to induce genotoxicity. The mice were administered 0.2 mL of extract per day for 10 days in the genotoxicity and antigenotoxicity groups. Administration of each of the extracts at the concentration of 5 000 mg/kg did not induce acute toxicity in mice. At the concentrations tested, all the extracts, except aqueous S. mombin, increased micronucleated polychromatic erythrocytes. The aqueous and hydro-methanol extracts of N. lotus increased the frequency of aberrant sperm cells. All the extracts were also able to ameliorate MMS induced genotoxicity in bone marrow cells of the exposed mice. The results showed the potential of the extracts to induce somatic and germ cell mutation in male mice. The extracts also ameliorated the genotoxic effect of MMS.