Scrotal hemangiosarcoma in a Large White boar

ABSTRACT: Tumors are rarely diagnosed in swine specie because of the short lifespan of production animals. Normally, these tumors do not present any clinical signs and are often detected at the time of slaughter. A 2-year-old Large White boar, used in the reproductive management of a farm and without a history of pre-existing problems, was examined for skin lesions on the scrotum. Samples were collected from skin segments containing lesions for histopathological and immunohistochemical diagnosis. Microscopically, the nodes in the scrotum pouch consisted of poorly demarcated, highly cellular, expansile, and multifocally invasive neoplasms, composed of immature endotheliocytes organized into neovascular formations. The tumor cells were pleomorphic, slightly oval to spindle-shaped, with eosinophilic cytoplasm and hyperchromatic nuclei with one to three nucleoli. All the nodules analyzed were compatible with hemangiosarcoma. After immunohistochemical evaluation, for the quantification of tissue angiogenesis the neoplastic cells immunoexpressed the CD31 monoclonal antibodies and factor VIII, through the identification of proteins expressed on the surface of endothelial cells. The Ki67 cell proliferation marker was positive in approximately 10% of the neoplastic cells, demonstrating a high degree of malignancy. Hemangiosarcoma in swine species has already been identified in several organs and tissues; however, to date, no study has demonstrated the diagnosis of this condition on the skin of the scrotum, as reported in this study. Therefore, it is expected that this report will contribute to the knowledge of the frequency of neoplasms in swine species.

Systemic and Craniospinal Rosai Dorfman Disease with Intraparenchymal, Intramedullary and Leptomeningeal Disease

Rosai Dorfman disease is a rare histiocytic disorder of over-production of non-Langerhans histiocytes, which typically manifests with massive lymphadenopathy and sinonasal involvement. We report a rare case of systemic and disseminated craniospinal Rosai-Dorfman disease with intraparenchymal and leptomeningeal involvement, but no sinus or dural-based disease. The diagnosis was established by biopsy of a hypothalamic mass. Additionally, UCSF500 Next Generation Sequencing demonstrated a solitary pathogenic alteration affecting the BRAF oncogene, which supports the morphologic and immunohistochemical diagnosis of Rosai-Dorfman disease.

Immunohistochemical diagnosis of onychomycosis by monoclonal antibodies detection of dermatophyte T. rubrum

Introduction/Objective Onychomycosis is one of the most common nail infection disorders, caused predominantly by T. rubrum. Currently, PAS stain is the gold standard for histological onychomycosis detection. However, it does not differentiate between the types of fungi. In addition, PAS stains will only provide a positive or negative result. This leaves physicians to prescribe medications that may be ineffective in treating the root cause of the infection. By discerning the causative specie, physicians can provide a more targeted and effective anti-fungal therapy. A novel monoclonal antibody can be utilized to improve histological findings of T. rubrum. Our study takes a step forward to bring the monoclonal antibody into histological application. We have developed a new monoclonal antibody stain which binds to T. rubrum in vitro. For our project, we will perform in vivo staining on clinical nail samples using the novel monoclonal antibody. Developing a new applicable technique will benefit patients with onychomycosis as well as promote research in targeted identification of other infectious pathologies. Methods/Case Report Data from Bach Dx’s collaboration demonstrate isolation and validation of mice monoclonal antibody candidates for T. rubrum. Isotype IgG1/kappa 17B6 had the highest binding pair signal to the analyte. Direct ELISA of 17B6.1E3 shows immunoreactivity to T. rubrum. HRP and AP conjugates to 17B6 mice monoclonal antibody are produced (Rockland Immunochemicals Inc. Gilbertsville, PA). Histology slides analyzed for routine onychomycosis analysis at Bach Diagnostics are subject to secondary analysis. 220 retrospective, unstained nail slides from 55 patients will be stained using the 17B6 stains. 40 patients were known to have tested for onychomycosis caused by T. rubrum, 5 by Candida albicans, 5 by T. interdigitale, and 5 tested negative. All samples were confirmed by histology and molecular tests. H&E, PAS, and naked antibody stains will serve as controls. HRP and AP conjugated 17B6 monoclonal antibody stains will be optimized to Quantum Hdx (StatLab Lodi, CA) on July 12th, 2021 when the stains are scheduled to arrive. Images will be captured by light microscopy, and analyzed quantitatively and qualitatively. Results (if a Case Study enter NA) We hope to show preferential staining of antibody stains to positive T. rubrum cases by end of July 2021. Conclusion Conclusion pending based on results.

III all-Russian school-seminar For immunohistochemical diagnosis of tumors

The III All-Russian school-seminar on immunohistochemical diagnosis of tumors was held at the Clinical Oncological Dispensary (CODE) of the Ministry of Health of the Republic of Tatarstan. The school participants were 30 oncomorphologists from Moscow, Novosibirsk, Rostov-on-Don, Yekaterinburg, Krasnodar, Vitebsk, Perm, Tyumen, Khanty-Mansiysk, Chelyabinsk, Cheboksary, Ulyanovsk, Ufa, Khabarovsk, as well as pathologists of the Republic of Tatarstan.

Monoclonal Antibodies for Immunohistochemical Diagnosis of Breast Cancer

Breast cancer is a leading malignant disease in women worldwide, although its pathology is visually localised. Currently, it has been proven that the parameters of molecular genetic biomarkers, including oncoprotein HER2, proliferation markers Ki-67, oestrogen receptors ER, and progesterone receptors PgR, are associated with breast carcinogenesis and are a reflection of the biological aggression of the tumour. The significance of these biomarkers in signalling pathways and genetic mechanisms of carcinogenesis has been described, as well as the relationship between the expression levels of each biomarker and the tumour response to appropriate therapy. The primary antibody that imparts specificity to IHC is based on the monoclonal antibodies (mAbs) as the main immunoreagent that enables reliable identification of breast cancer cells. The most commonly used antibodies to molecular biomarkers for IHC were determined in accordance with indicators of laboratory use and efficiency (pass rate) of HER2, Ki-67, ER, PgR assessments in the NordiQC breast cancer module. The discovery of the complete structure of these biomarkers and the design of their domains and subdomains by genetic engineering methods enable the synthesis of effective monoclonal antibodies. Quantitative indicators of the expression levels of tumour biomarkers of breast cancer were determined using mAb, depending on epitope specificity and affinity.

Fibroblast-associated protein-α expression and BPV nucleic acid distribution in equine sarcoids

Sarcoids are the most common cutaneous tumor of equids and are caused by bovine papillomavirus (BPV). Different clinical subtypes of sarcoids are well characterized clinically but not histologically, and it is not known whether viral activity influences the clinical or histological appearance of the tumors. The aim of this study was to verify whether the development of different clinical types of sarcoids or the presence of certain histological features were associated with BPV distribution within the tumor. The presence of BPV was assessed by polymerase chain reaction (PCR) and visualized in histological sections by chromogenic in situ hybridization (CISH) in 74 equine sarcoids. Furthermore, to better characterize the molecular features of neoplastic cells, immunohistochemistry for S100, smooth muscle actin-α (αSMA), and fibroblast-associated protein-α (FAPα) was performed. The presence of BPV was confirmed in all tissues examined by either or both PCR and CISH (72/74, 97% each). Of 70/74 CISH-positive cases, signal distribution appeared as either diffuse (61/70, 87%) or subepithelial (9/70, 13%); the latter was more frequently observed in the verrucous subtype. However, no statistically significant association was found between clinical subtypes and specific histological features or hybridization pattern. Moreover, CISH signal for BPV was not detected in the epidermis overlying sarcoids nor in the tissue surrounding the neoplasms. By immunohistochemistry, αSMA confirmed the myofibroblastic differentiation of neoplastic cells in 28/74 (38%) sarcoids. Using tissue microarrays, FAPα labelling was observed in neoplastic fibroblasts of all sarcoids, suggesting this marker as a potential candidate for the immunohistochemical diagnosis of sarcoids.

Case Report: Fibrosarcoma in Maxilla in Youngest

Abstract- Fibrosarcomas are rare but highly fulminant and aggressively malignant neoplasm whichoccurs as a result of mutated spindle-shaped fibroblasts. According to WHO, they are soft tissuesarcoma commonly present seldom at cutis. The etiology of such a malignancy is obscurednevertheless it has several predisposing factors like pre-existing pathology but the commonest ofthem is radiation. Mesenchymal malignancies were initially over-diagnosed and were the epicenter ofdiagnostic dilemma to the pathologist, but with the advent of Vimentin staining andimmunohistochemistry, the diagnostic front has fairly advanced for fibrosarcomas. Incidence ofFibrosarcoma in Maxilla accounts for less than 0.1%, when compared to other head and neckmalignancies, and is predominantly found between 2nd to 6th decades of life. Various approacheshave been documented in the literature regarding its treatment, but aggressive surgical excisionremains the mainstay of treating Fibrosarcomas. The role of adjuvant medicinal therapies have beenwell debated but do have a conclusive outcome. Through this article, we want to highlight anddocument a case, which occurred at an exceptionally young age, at a site that had no predisposingfactors but has proven immunohistochemical diagnosis and was rapidly fulminating and aggressivelymalignant. The preoperative diagnosis of the patient was inconclusive reporting it to be amesenchymal tumor, therefore, the patient was treated with the intraoral approach for wide surgicalresection. The surgical specimen was subjected to immunohistochemistry with Vimentin stainingwhich reported it to be a Fibrosarcomas, therefore the patient was advised chemotherapy, yet theprognosis is guarded.