Using Adaptive Capacity to Shift Absorptive Capacity: A Framework of Water Reallocation in Highly Modified Rivers

Damming and water regulation creates highly modified rivers with limited ecosystem integrity and resilience. This, coupled with an ongoing global biodiversity crisis, makes river restoration a priority, which requires water reallocation. Coupled human–natural systems research provides a suitable lens for integrated systems’ analysis but offers limited insight into the governance processes of water reallocation. Therefore, we propose an analytical framework, which combines insight from social–hydrological resilience and water reallocation research, and identifies the adaptive capacity in highly modified rivers as the capacity for water reallocation. We test the framework by conducting an analysis of Sweden, pre- and post-2019, a critical juncture in the governance of the country’s hydropower producing rivers. We identify a relative increase in adaptive capacity post- 2019 since water reallocation is set to occur in smaller rivers and tributaries, while leaving large-scaled rivers to enjoy limited water reallocation, or even increased allocation to hydropower. We contend that the proposed framework is broad enough to be of general interest, yet sufficiently specific to contribute to the construction of middle-range theories, which could further our understanding of why and how governance processes function, change, and lead to outcomes in terms of modified natural resource management and resilience shifts.

Integrated Systems Analysis of Mixed Neuroglial Cultures Proteome Post Oxycodone Exposure

Opioid abuse has become a major public health crisis that affects millions of individuals across the globe. This widespread abuse of prescription opioids and dramatic increase in the availability of illicit opioids have created what is known as the opioid epidemic. Pregnant women are a particularly vulnerable group since they are prescribed for opioids such as morphine, buprenorphine, and methadone, all of which have been shown to cross the placenta and potentially impact the developing fetus. Limited information exists regarding the effect of oxycodone (oxy) on synaptic alterations. To fill this knowledge gap, we employed an integrated system approach to identify proteomic signatures and pathways impacted on mixed neuroglial cultures treated with oxy for 24 h. Differentially expressed proteins were mapped onto global canonical pathways using ingenuity pathway analysis (IPA), identifying enriched pathways associated with ephrin signaling, semaphorin signaling, synaptic long-term depression, endocannabinoid signaling, and opioid signaling. Further analysis by ClueGO identified that the dominant category of differentially expressed protein functions was associated with GDP binding. Since opioid receptors are G-protein coupled receptors (GPCRs), these data indicate that oxy exposure perturbs key pathways associated with synaptic function.

Integrated systems analysis reveals conserved gene networks underlying response to spinal cord injury

Spinal cord injury (SCI) is a devastating neurological condition for which there are currently no effective treatment options to restore function. A major obstacle to the development of new therapies is our fragmentary understanding of the coordinated pathophysiological processes triggered by damage to the human spinal cord. Here, we describe a systems biology approach to integrate decades of small-scale experiments with unbiased, genome-wide gene expression from the human spinal cord, revealing a gene regulatory network signature of the pathophysiological response to SCI. Our integrative analyses converge on an evolutionarily conserved gene subnetwork enriched for genes associated with the response to SCI by small-scale experiments, and whose expression is upregulated in a severity-dependent manner following injury and downregulated in functional recovery. We validate the severity-dependent upregulation of this subnetwork in rodents in primary transcriptomic and proteomic studies. Our analysis provides systems-level view of the coordinated molecular processes activated in response to SCI.