In 2017, a new race (TSA-6) of the wheat stripe rust pathogen, Puccinia striiformis f. sp. tritici, virulent to resistance gene Yr5 were detected in China. However, whether Chinese wheat cultivars are resistant to the new races was unknown. In this study, two isolates (TSA-6 and TSA-9) with virulence to Yr5 were tested on other wheat Yr gene lines for their avirulence/virulence patterns and used, together with prevalent races CYR32 and CYR34 without the Yr5 virulence, to evaluate 165 major Chinese wheat cultivars for their reactions. Isolates TSA-6 and TSA-9 had similar but different virulence spectra, and therefore should be considered as two different races. Their avirulent/virulence patterns were remarkably different from that of CYR34 but quite similar to that of CYR32. Of the 165 wheat cultivars, 21 had all-stage resistance to TSA-6, 34 to TSA-9, and 20 to both races. Adult-plant resistance (APR) was detected in 35 cultivars to TSA-6 and 27 to TSA-9, but only 3 cultivars showed APR to both new races. Slow rusting resistance was observed in 24 cultivars to TSA-6 and of 33 to TSA-9. Analysis of variance (ANOVA) of disease index indicated a significant difference among cultivars, but not among the four races. Based on the molecular marker data, a low percentage of wheat cultivars carried Yr5, Yr7, Yr10, Yr15, Yr26, and/or YrSP. As TSA-6 and TSA-9 can be a serious threat to wheat production in China, monitoring TSA-6, TSA-9, and other races are continually needed.