Effects of Anti-Sickling Polyherbal Mixture on Haematological Indices in Normal and Anaemia-Induced Rabbits

Some plants used in the management of sickle cell disease (SCD) have been shown to increase gelling time of sickle cell blood and inhibits sickling in vitro, reversal of sickling, inhibiting osmotically induced haemolysis of erythrocytes, membrane stabilization. Plants such as Sorghum bicolar, Phyllanthus amarus, U. afzelii, Securidaca longipedunculata, Momordica charantia, Dalium guineense have been found to exhibit anti-sickling properties. The polyherbal combination of these drugs was used in this study for the investigation of the effects of anti-sickling polyherbal mixture on haematological indices in rabbits. Sixty (60) New Zealand rabbits weighing 1200g ±200g, conducted in duplicate and designated experiment X (normal rabbits) and Y (anaemia induced using Cadmium 2mg/kg +10 mg/Kg body weight phenylhydrazine for 15 days). Both groups were further subdivided into four groups (A-D) with 5 animals each, administered saline substitute, different grades of polyherbal mixtures for 8 weeks. Blood sample (2mls) was collected into Ethylene Diamine Tetra Acetic Acid (EDTA) bottles for full blood count. The results in the experiment (X) showed significant increase in WBC (103/μl) count across the groups (B=8.15±0.33; C=9.3±0.74; D=9.8±0.59) compared to the control group (A=7.25±0.44) (p<0.05). RBC (106/μl) count showed insignificant increase in group B (5.1±0.20) and C (5.6±0.450), decrease level in D (4.4±0.26) compared to control A (4.8±0.29) (p<0.05). Hb(g/dl) and HCT (%) showed similar pattern with insignificant increased levels across all treatment groups (B=14.8±0.59, 43.4±1.74; C=15.5±0.78, 45.8±3.; D=14.2±0.85, 43.7±2.62) compared to control A (13.6±0.68; 41.5±2.49) respectively. Platelets showed significant increase in group B and C (286±17.16 and 286±17.16) compared to control A (244±9.76) with significant decrease in group D (226±18.08) (p<0.05). In experiment (Y), WBC count showed significant increase across all treatment groups (B= 6.4±0.26 C= 6.8±0.54 D =10.6±0.64) compared to control (A= 4±0.24) (p<0.05). RBC (106/μl) count in control A gave (2.5±0.15), insignificant increase in B (3.1±0.12) with significant increase in group C (3.5±0.28) and insignificant decrease in D (2.2±0.13) (p<0.05). Higher level of Hb (g/dL) was seen in group B and C (B= 8.4±0.34; C= 9.1±0.46), while D (7.7±0.46) had a slightly elevated value compared to the control A (7.1±0.36) (p<0.05). HCT (%) showed significant increase across the groups (B=26±1.04; C=28±2.24; D=24±1.44) (p<0.05) compared to control A (18±1.08). Red cell indices showed some degree of derangement across the study groups. Findings in this study suggest that polyherbal mixture have a positive effect on the haemoglobin, red blood cells, packed cell volume and white blood cell count of the rabbits in a dose dependent manner.

Clinico-toxicological effects of ceftriaxone after intramuscular administration of graded doses in Basenji dogs

Purpose: The recent ceftriaxone-induced anaemia and mortalities at the dose of 50 mg/kg in Veterinary Teaching Hospital, University of Nigeria prompted this study which sought to assess the clinicotoxicological effects of ceftriaxone (CFZ) after intramuscular administration of graded doses in Basenji dogs.Methods: The effects of CFZ on the haematological indices, physiological parameters, liver and kidney functions were assessed in 4 group of dogs (n = 4) designated A – D. They were given CFZ intramuscularly for 21 days at doses of 12, 25 and 50 mg/kg for groups A, B, C, respectively, while thecontrol (group D) received the diluent (lignocaine 0.2 mL)Results: The mean pulse and heart rate of dogs in group C were significantly (p < 0.05) higher than those of group A, B and D. Significant (p < 0.05) decrease in red blood cell count (RBC), haemoglobin concentration (Hb) and packed cell volume (PCV) was observed in group C on days 7 and 14, while on day 21, these parameters were significantly (p < 0.05) higher in group D than in the treated groups. On day 14 of CFZ administration, the alanine transaminase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities of dogs in group C was significantly (p < 0.05) elevated than the control group.Conclusion: These findings suggest that CFZ, at the doses of 12.5 - 25 mg/kg, appears safe in dogs as most of the adverse effects observed are reversed following the withdrawal of the drug on day 28. However, CFZ at 50 mg/kg causes anaemia, tachycardia and bilateral paralysis of the hind limbs which did not revert to normal after one week; hence, it is not recommended for use in dogs at this dose.

Lipid Profile, Haematological Assay and Tissue Histology of Alloxan Induced Diabetic Wistar Rats Administered Extracts of Vernomia amygdalina (Bitter leaf) and Gnetum africanum (okazi leaf)

Diabetes mellitus is predominant in numerous nations of the world with millions of deaths directly linked to it. The utilization of plants in providing answers to this pandemic has expanded over the years. This study investigated changes in some haematological parameters and tissue histology of alloxan induced diabetic Wistar rats administered combined leaf extracts (CLE) of vernonia amygdalina (bitter leaf (BI)) and gnetum africanum (okazi leaf (OK)). Aqueous extracts of bitter leaf and okazi leaf were prepared using the conventional method. Forty Wistar rats were grouped into eight of five rats each. Groups A and B were normal and diabetic control respectively, groups C to G (diabetic groups) were treated with varied mixtures of extracts of vernomia amygdalina and gnetum africanum at (10:90BI/OK), (30:70BI/OK), (50:50BI/OK), (70:30 BI/OK) and (90:10% BI/OK) ratios respectively. Group H, the diabetic control was administered the standard drug (Metformin). The animals were sacrificed on the 28th day, blood samples and liver tissue were collected for biochemical analysis and histological examination. There was a significant (p<0.05) reduction in the lipid profile of the diabetic groups (C-G) especially in triglycerides with the highest reduction in (50:50 BI/OK) combination (0.70±0.07) while the least reduction was seen in (30:70 BI/OK) combination (1.37±0.08). There were time and ratio-dependent variations in the haematological indices. Hepatic histology showed evidence of varying levels of restoration of cellular structural integrity by the combined extracts. These results suggest that the combined extracts of vernomia amygdalina and gnetum africanum could be used to manage diabetes mellitus.

Effect of Fluoride Toxicity on Some Haematological Indices in Human Population

The present study aimed to determine the toxicity of Fluoride on haematological indices in some population residing in fluoride endemic area of Dongergaon village of Chandrapur district, Maharashtra, India. The study group was selected on the basis of elevated level of serum fluoride and symptoms of skeletal fluorosis. Various haematological parameters such as Hb, TEC, TLC, HCT and PLT count were analysed. Students t-test was applied for statistical analysis and the result showed that values of haematological indices in study group were significantly declined as compared to control group. The outcomes of the present study indicated the reduction in Hb, TEC, HCT, increase in TLC and non-significant change in PLT.

Assessment of the growth performance and haematological indices of Clarias gariepinus fingerlings exposed to soap effluent

Background Various substances released from modern complex human societies which enter the aquatic ecosystems produce alterations in survivability of aquatic biota. This study assesses the consequence of exposure to industrial effluents containing potentially hazardous constituents, especially soap industrial waste effluent, relative to fish physiology, growth and survival, particularly food fish such as Clarias gariepinus. Five hundred (500) fingerlings of C. gariepinus (15.65 ± 0.02 g) randomly stocked at 50 fish per tank in duplicates were exposed to varying concentration (0, 0.32, 0.66, 1.31 and 2.63%) of homogenous effluent sample and fed Durante® floating feed at 5% of the body weight in two instalments per day for 180 days. The growth performance and feed utilization data were generated to determine the growth performance indices. The haematological parameters of the fish were also determined following routine methods of fish haematology. Result Generally, fish in the control had significantly (p < 0.05) better growth performance indices compared with the exposed fish. It was also observed that most of the significant increase or decrease observed in the growth performance was concentration dependent. Increase in HES concentration which significantly increases (p < 0.05) WBC count of the experimental fish was observed to decrease (p < 0.05) the HGB level of the fish. Significantly higher (p < 0.05) levels of RBC (3.02 ± 0.03 × 106/µL) and HCT (36.70 ± 1.68%) were recorded in the control fish. However, all the exposed fish had significantly higher (p < 0.05) levels of platelet count compared with the control. Conclusion This study concluded that exposure to concentrations of soap effluent induced stress, inhibited growth and altered the haematological indices of the exposed fish. Uncontrolled discharge of soap effluent into receiving water bodies, apart from affecting the wellbeing of an important freshwater food fish, there is every probability of possible accumulation of the chemical/toxic additives of the soap effluent which could have serious implications considering the man as the major and final recipient of these toxic bioaccumulated chemicals via the food chain and the environment.

Effect of Solanum gilo leaf extract on some haematological indices of albino Wistar rats

Solanum gilo (eggplant) is an edible vegetable whose leaf is reported to possess numerous medicinal properties due to its rich constituents. Against this backdrop, there is possibility that its consumption may have effect on haematological indices. Therefore this research sought to assess the effect of Solanum gilo leaf diet on some haemotological indices of albino wistar rats. About twenty four (24) albino wistar rats weighing between 120-150mg were randomly assigned into four groups of six animals each. Each rat was allowed to drinking water ad labitum. Control group received normal rodent chow while group 2 was fed 5% Solanum gilo diet. Group 3 was fed 10% Solanum gilo diet and group 4 received 20% Solanum gilo diet. The different diets were prepared by mixing different proportions of ground S. gilo leaves with rodent chow. Automated blood cell analyzer (Model PCE 210, Japan) was used for analysis of haematological parameters. Preliminary study on its proximate composition, showed the appreciable presence of crude fat, protein, fibre and carbohydrate. Consumption of S. gilo caused significant improvement in some blood parameters; notably packed cell volume (PCV), white blood cell (WBC) and platelet counts. Hence, eggplant is a nutritious vegetable with healing, the therapeutic and immunity properties. If exploited in human diet has the potentials of improving quality of life.

Effect of processed doum palm (Hyphaene thebaica) pulp meal on growth performance, nutrient digestibility, carcass characteristics and haematological indices of broilers

The increase in human population, particularly in the developing countries like Nigeria and the demand for animal protein intake has necessitated different approaches of solving protein shortage. A 28-day study was carried out to determine the performance of finishers fed on different hourly soaked doum palm (Hyphaene thebaica) pulp meal (DPPM) diets. Two hundred and sixteen (216) finishers (4 week-old) were randomly allotted to six dietary treatments. Each treatment consisted of 36 birds with three replicates (12 birds per replicate) in a completely randomized design (CRD). Six experimental diets were formulated and designed as follows: T1 (Control) contained 0% DPPM while T2, T3, T4, T5 and T6 contained 12.5% each of 2, 4, 6, 8 and 10 hours soaked DPPM as substitute for maize, respectively. Feeds and water were provided ad libitum for all treatment groups. The average body weight gain (40.44- 42.71 g/b/d), average feed intake (87.52-96.73 g/b/d) and feed conversion ratio (2.14-2.34) were non-significant (p>0.05) regardless of the dietary treatments. Statistical differences (p<0.05) were observed in some carcass parameters such as thigh (13.40-14.98%), neck (5.32-6.55%), shank (3.81-5.2 %), wing (9.80-11.41%), head (2.43-3.74%) and two haematological indices; white blood cell (160.67-189.53 X 103/mm3) and eosinophils (0.33-2.00%) across the treatment groups. The processed DPPM could serve as a rich energy source in broiler feeds with the raw doum palm pulp containing 2987.45 kcal/kg. Also, soaking the pulp for 2 to 10 hours before been included in broiler diet improved the nutritional content, acceptability, palatability and utilization of the feed by the broilers. Thus, up to 12.5% processed DPPM can be included in the diets of broiler chickens without adverse effect on growth performance, nutrient digestibility, carcass characteristics and haematological indices of chickens.

BIOCHEMICAL AND HAEMATOLOGICAL CHANGES ASSOCIATED WITH TRANSPLACENTAL CONGENITAL MALARIA IN KOGI STATE, NORTH CENTRAL NIGERIA

Background: Transplacental congenital malaria is a vertical transplacental transmission of malaria parasites from the mother to the baby in utero or perinatally during labor. Cord blood that conveyed oxygen and nutrients from mother to fetus and return with carbon dioxide and other waste materials can transmit malaria pathogen. This study is aim to establish early diagnosis of transplacental congenital malaria using cord blood biochemical and haematological indices. Cord blood from 164 babies delivered at three hospitals in Kogi State between January and December, 2020 were microscopically investigated for malaria parasite. Biochemical and Haematological analyses were done using SYSMEX XP 300, Roche 9180 and VIS Spectrophotometer model 721. The data obtained were expressed as mean plus or minus standard deviation using SPSS 23. The indicator level of statistical significance was set at p<0.05. The results showed significant (p<0.05) decreased in values of WBC, platelet, sodium, potassium, urea, creatinine, RBC, PCV, haemoglobin and MCHC in malaria infected cord blood in comparison to malaria negative control group. Significant (P<0.05) increased activities of liver enzymes (AST, ALT, ALP), total protein, bicarbonate and chloride in malaria infected cord blood when compared with malaria negative group. However, no statistically significant difference in lymphocyte, MCV, MCH, neutrophil and mixed of both malaria infected and malaria negative cord blood. This study suggests that cord blood biochemical and haematological indices can be used to diagnose and manage transplacental congenital malaria in fetus and neonates. Keywords: Transplacental, Biochemical, Haematological and Congenital Malaria.