Study of Cananga odorata (Lam.) Hook. f. & Thoms. Flower Development: Morphological Variations in an Urban Environment

Cananga odorata is a native plant in the Indonesian archipelago. The flowers are often used to produce essential oils with many uses and a distinct fragrance. This study aims to observe each stage of the Cananga odorata flower development. The flowers were obtained from a home garden in Pasar Minggu, South Jakarta, from November 2020 until January 2021. Further observations of the stamen and pistil developments were conducted using Dino-Lite Edge Digital Microscope AM4115 Series. The results show that Cananga odorata flower development can be categorized into bud, display-petal, initial-flowering, full-flowering, end-flowering, and senescence stages. The flowers require 35 days to develop from bud stage to flower senescence. Stamens and pistils also develop primarily during the bud stages and mature after flower anthesis. Flower mutants were also found and may be caused by a mutation in the flower’s homeotic genes. Each different stages of flower development show a different morphological change in the flower perianth and reproductive organs. A discrepancy of flower morphology within each stage, especially those seen during the anthesis stages, might imply a variation in the flower’s internal factors.

Phenol Coefficient Test Combination Infusion of Cananga odorata – Averrhoa bilimbi L. Against Staphylococcus aureus and Salmonella typhi in Vitro

Cananga odorata (kenanga) flowers and Averrhoa bilimbi L. (belimbing wuluh) fruit are plant parts that contain relatively the same antibacterial compounds, namely flavonoids, saponins, and tannins. Both of these plants can be developed as an alternative natural antiseptic preparation, which is made in the form of a combination infusion. The effectiveness of an antiseptic preparation is measured by the coefficient value of the phenol antiseptic substance compared to 5% phenol against several standard test bacteria. This study aims to determine the antibacterial activity of the combination infusion of Cananga odorata flower - Averrhoa bilimbi L. fruit against Staphylococcus aureus ATCC 25923 and Salmonella typhi ATCC 19430 based on the in vitro phenol coefficient test. The phenol coefficient test method was carried out conventionally. The coefficient value close to 1 can be said that the antiseptic preparation has good activity and is equivalent to the phenol coefficient value as a comparison. The results showed that the mean coefficient of the phenol coefficient of the combination infusion of Cananga odorata flower - Averrhoa bilimbi L. fruit was 0.91 against S. aureus and 0.83 against S. typhi. The conclusion of this study, the antibacterial activity of the combination infusion of Cananga odorata flower - Averrhoa bilimbi L. fruit against Staphylococcus aureus obtained a higher phenol coefficient value than Salmonella typhi.

Cananga odorata (Magnoliales: Annonaceae) Essential Oil Produces Significant Avoidance Behavior in Mosquitoes

Essential oil of Cananga odorata Hook. F. & Tomson is a source of insect repellent, but contact irritancy and noncontact repellency actions that stimulate insect’s avoidance behavior (escape away from chemical source after direct physical contact or without making physical contact, respectively) have not been investigated. Therefore, an excito-repellency test chamber was used for measuring avoidance behavior of four insectary-reared mosquito species (Diptera: Culicidae) that escape from esposure to four concentrations (0.5, 1.0, 2.5, and 5.0% v/v) of C. odorata oil. The oil strongly repelled both Culex quinquefasciatus Say (85–97% escape) and Anopheles minimus Theobald (97–99%) at high concentrations (2.5–5.0%). For Anopheles dirus Peyton & Harrison and Aedes aegypti (L.), highest repellency (64 and 39% escape, respectively) was demonstrated at 2.5% concentration. For contact irritancy, the oil produced relatively high percent escape found in Cx. quinquefasciatus (90–100% escape) and An. minimus (83–100%). Whereas moderate contact irritancy was observed against An. dirus (40–50% escape) and Ae. aegypti (51–59%). The percent escape was then adjusted with repellency to estimate the effect of contact irritancy alone. We found that highest contact irritancy was presented at 0.5% concentration against An. minimus (67% escape). Knockdown and toxic actions were only found in Anopheles mosquitoes at 5.0% concentration. The results revealed that An. minimus and Cx. quinquefasciatus were more prone to be repelled by C. odorata oil. Detailed analysis of oil identified primary compounds as methyl benzoate (14.6%), α-gurjunene (12.8%), p-methyl-anisole (11.3%), and benzyl acetate (9.9%). Further investigations are needed to assess excito-repellency actions of these compounds alone or in combination.

Antibacterial and Mode of Action of Extracts from Endophytic Fungi Derived from Terminalia mantaly, Terminalia catappa, and Cananga odorata

Emerging drug-resistant bacteria creates an urgent need to search for antibiotics drugs with novel mechanisms of action. Endophytes have established a reputation as a source of structurally novel secondary metabolites with a wide range of biological activities. In the present study, we explore the antibacterial potential of endophytic fungi isolated from different tissues of Terminalia mantaly, Terminalia catappa, and Cananga odorata. The crude ethyl acetate extracts of 56 different endophytic fungi were screened against seven bacterial strains using the broth microdilution method. The antibacterial modes of action of the most active extracts (04) were evaluated using E. coli ATCC 25922 and H. influenzae ATCC 49247 strains. Both the DPPH and FRAP assays were used to investigate their antioxidant activity, and their cytotoxicity against the Vero cell line was evaluated using the MTT assay. Out of the 56 crude extracts tested, about 13% were considered very active, 66% partially active, and 21% nonactive against all tested bacterial strains with MIC values ranging from 0.32 μg/mL to 25 μg/mL. The four more potent extracts (MIC <5 μg/mL) (from Aspergillus sp. N454, Aspergillus sp. N13, Curvularia sp. N101, and Aspergillus sp. N18) significantly lysed the bacteria cells, increased outer membrane permeability, reduced salt tolerance, and inhibited bacterial catalase activity. They exhibited a DPPH free radical scavenging activity with I C 50 ranging from 150.71 to 936.08 μg/mL. Three of the four potent extracts were noncytotoxic against the Vero cells line ( C C 50 > 100 μg/mL). Results from this investigation demonstrated that endophytes from Cameroonian medicinal plants might content potent antibacterial metabolites. The bioguided fractionation of these potent extracts is ongoing to isolate and characterise potential active ingredients.

The Effect of Cananga (Cananga odorata) Aromatherapy on Anxiety Level of Patients Before Tooth Extraction in RSGM-P USU

Tooth extraction is a form of treatment in dentistry. Tooth extraction has the potential to cause excessive anxiety in patients because patients tend to think about things that will or can happen during the procedure. The purpose of this study was to determine the effect of Cananga aromatherapy (Cananga odorata) on the anxiety level of patients at RSGM USU. This research method is a quasi-experimental study using a re-experimental design non-randomized pretest and posttest control group design. The sample was divided into 2 groups, namely the treatment group and the control group. Each group consists of 16 people who will extract the posterior teeth of the upper and/or lower jaw. The treatment group was given a Cananga aromatherapy nasal inhaler and the control group was given an empty nasal inhaler without Cananga aromatherapy. Blood pressure and pulse measurements were taken twice. The first measurement after the patient was given informed consent and the MDAS questionnaire (Modified Dental Anxiety Scale) and the second measurement after inhaling the Cananga aromatherapy nasal inhaler and empty nasal inhaler without Cananga aromatherapy within 10 cm from the nasal cavity for 5 minutes. The results of this study were analyzed using the Wilcoxon and Mann-Whitney tests. In the treatment group, there was a significant decrease in blood pressure and pulse compared to the control group with a value of p = 0.000 <0.005. This study concludes that Cananga aromatherapy is effective in reducing the anxiety level of patients before tooth extraction in RSGM USU.

Flowers and Value of Conservation in The Culture of Hindu Community in Bali

Balinese Hindu community cannot be separated from plant in their life. The information on plant species and how to utilize them are easy to find but limited information about the potency of flowers in the Balinese Hindu community. Flowers have a meaning as sincerity, a symbol of Sang Hyang Widhi, the valour, and also have mythological values. This research aimed to determine the species of flowering plants that are often used for ceremonial and understand the meaning, the cultural conception related to conservation education. The study was carried out in four locations in Tabanan Regency (Baturiti, Penebel, Marga, and Tabanan). Data were collected using an interview with purposive sampling. Data analysis based on the value of Relative Frequency (FR) which is indicated plant most widely used. Â A total of 36 species of flowers are used in traditional Hindu religious ceremonies, of which 29 species are cultivated plants and 7 species are still wild. There are ten of plants which have high FR value, such as: Cananga odorata (FR 0.069), Impatiens balsamina (FR 0.069), Hydrangea macrophylla (FR 0.069), Plumeria acuminata (FR 0.069), Tagetes erecta (FR 0.069), Magnolia champaca (FR 0.069), Gomphrena globosa / bunga ratna (FR 0.047), Gardenia jasminoides / jempiring (FR 0.039), Nymphaea spp./ tunjung (FR 0.030), and Hibiscus rosa-sinensis L./ pucuk (FR 0.030). This research revealed the role of flower in Hindu ceremony and describe the strategic plant conservation due to culture of Balinese Hindu community. Finally, this research expected to documented local knowledge about flowers used as Hindu religious ceremonies, to provide an understanding of the meaning and conceptions of culture related to conservation education.

Cananga odorata (ylang-ylang).

Cananga odorata is known as the perfume tree or ylang-ylang, and the flowers (also leaves and fruits) yield an important essential oil widely used in perfume manufacture. The essential oil also has a wide range of medicinal uses. Cananga odorata can grow to a height of about 30 m with a straight stem and drooping, brittle branches, but when grown for perfume extraction it is normally not more than 3 m high. The tree is native to Southeast Asia, however, it has been widely planted, mainly in home gardens, as an ornamental, multipurpose species. It is found naturalized or planted in many tropical and subtropical areas of the World (particularly as an occasional species in fallow forests, open forests and along forest margins), including China, Taiwan and many of the Pacific Islands. The timber is used locally for construction purposes, for canoes, and occasionally for fuelwood. The species is also known to be sold and used for folk medicine. Medicinal uses include treatment of boils, as a carminative, for treatment of cephalgia, diarrhoea, gout, malaria, eye problems, rheumatism and as an emmenagogue (Duke, 2014).